| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,044,421 | A→C | 31.7% | C122W (TGT→TGG) | U712_15635 ← | Putative aminotransferase YugH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,044,421 | 0 | A | C | 31.7% | 30.9 / 5.3 | 34 | C122W (TGT→TGG) | U712_15635 | Putative aminotransferase YugH |
| Reads supporting (aligned to +/- strand): ref base A (15/8); new base C (2/9); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGAATGGCCTTTGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAACCTGTTGCTCAAAT > CP006881/3044286‑3044557 | gagctgggCCTTGGTTTTTTCTGCGACGGCCGCTTCAAAATCCGCAGCCGTTGCTTTAAATCCTTTGTCTGCGGTGTTATGACCGGC‑ACCCGGCAACCCGCCTGCCAAACAACCACGCGCATGGACCCCAACAAAcca < 4:99429/132‑1 (MQ=255) aaTGGCCTGTGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AGCCGGGATTCCGCCTGCCAAAGAAACAAGCGCATCGTACTCAACAAAACACGGc > 2:9177/1‑139 (MQ=255) gCCTTTGTTTTTTCAGTGACTGCCGCTTCAAAATCCGCAGCCGTTGCTTTAAATCCTTTGGCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCa < 7:227837/138‑1 (MQ=255) ccTTTGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTGTGCCGTGGTATGACCGTG‑AACCGGGATCCCGCCTGCCAAAGAACCACGCGCAGCGTACGCACCAAACCACGGCTCAg > 3:150261/1‑138 (MQ=255) ccTTTGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACCCGGCTCAg > 2:138611/1‑138 (MQ=255) tttGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGaa > 7:126984/1‑139 (MQ=255) tacAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAACCACGGCTCAGGAATGATGACt > 6:117850/3‑139 (MQ=255) aGTGACTGCCGCTTAAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTGTGCCGTTGTATGAACGGG‑ACCCGGGATACCGCCTGCCAAACAAACAAGCGCATCGTACGCAAAAAAACACGGCTCAGGAATGATGACTTc < 8:161067/138‑1 (MQ=255) tGCCGATTCAAAATCAGCAGGCGTTGATTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAATAAACAACCGGATAGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCt < 8:243894/139‑1 (MQ=255) cTTCAAAATCTGCAGCCGTTGCTTTAATTCCTTGGTCTGCCTTTGTATCAA‑GTGTACCCGGGATACCGCCGGCAAAAGAAACAAGCGCATCGTCCGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGAtt < 8:210722/139‑1 (MQ=255) agccgcGGTTGATTTAAAGAATTTGTCTGCCGTTGTATGAAGGTG‑CACCGGCAAACCGCCTCCCAAAGAAACACGCGCATCGTCCGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGgcg < 2:216205/139‑1 (MQ=255) gcgtttGGTTTAAATCGTTTGTGTGCGTGTGTATGATGGTG‑ACCCGGGATCCCGCCTGCAAACTAAACACGCGAATCGAACTCAAAAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGa < 8:83543/135‑1 (MQ=255) tGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATTCCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGAAGACTTCCTCCCCTGGAGTCACAATGGCGCGGTTCGc > 8:307794/1‑138 (MQ=255) gCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCa < 7:239837/138‑1 (MQ=255) gCTTTAAATCCTTTGGCTGCCGTTGTATGAACGTG‑AACCGGTATACCGCCTCCCAAAGAACCAAGCGCATCGTACGCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCa < 3:274272/138‑1 (MQ=255) gCTTAAAATCTTTTGTGTGCGGTTTAATGACCGTG‑ACCCGGGAACCCGCCTCCAACAGAAACAACCGCATCGAACGAAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCaa < 5:311744/139‑1 (MQ=255) aaaTCCTTTGTCTGCCGTTTTATCAACGTG‑ACCCGGGATCCCGCCTGCAAAACAACCACGCGCAGCGACCCCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATcc < 5:338290/139‑1 (MQ=255) tttGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGCCTCAGGAATGATGACTTCCTAACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGcc > 5:317455/1‑139 (MQ=255) gcTGCCGGTGTATGACCGGC‑ACCCGGGATCCCGCCTGCCAAACAAACACGCGCACCGCCCGCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGa < 4:150261/137‑1 (MQ=255) gggCCGTTGTAGGCACGTG‑AACCGGGATCCCGCCCGCCAAAGAAACCAGCGACTCGTCCGCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACt < 3:271933/137‑1 (MQ=255) ttGTCTGACCGTG‑TACCGCGATGCCGCCTCCCAAAGAACCACGCTCCTCGAACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGcccc < 8:294893/139‑1 (MQ=255) tATCAACGTG‑ACCCGGGAACCCGCCTGCCAAACAAACACGCGCATCGTACCAAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCa < 7:172134/138‑1 (MQ=255) tG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTcaca > 1:32213/1‑138 (MQ=255) aaCCGGGATACCGCCTGCCAAAGAGACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGACTGAATTGCCCCCACTGTcacatt > 2:153682/1‑136 (MQ=255) aaCCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAAt > 2:13169/1‑138 (MQ=255) cGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACCCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGc < 1:114410/139‑1 (MQ=255) ccTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGCTGGCTATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTg > 3:114960/1‑139 (MQ=255) ccTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTg > 6:286139/1‑139 (MQ=255) cATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCACCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAggctgtc > 5:194194/1‑134 (MQ=255) aTCGTACGCAACAAAACACGGCTGAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAAc > 2:186969/1‑139 (MQ=255) aTCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAAc > 1:13274/1‑139 (MQ=255) gaaCGCAACAACACACCGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAACCTg < 4:123676/137‑1 (MQ=255) cGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAACCTGTTg > 8:28333/1‑139 (MQ=255) gCAACAAACCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCaa < 1:89659/64‑1 (MQ=255) gCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCaa > 2:89659/1‑64 (MQ=255) aaCACGGCTCAGGAATGATGACTTCCTCCCCTGGATTCACAATGGCGCGGAGCTCAAGATCCAAAGCCTGACATGCCCCCACTTTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAACCCTGTTGATCaaag > 2:26385/1‑137 (MQ=255) aaCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAAt < 7:207980/91‑1 (MQ=255) aaCACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAAt > 8:207980/1‑91 (MQ=255) | GAGAATGGCCTTTGTTTTTTCAGTGACTGCCGCTTCAAAATCAGCAGCCGTTGCTTTAAATCCTTTGTCTGCCGTTGTATGAACGTG‑AACCGGGATACCGCCTGCCAAAGAAACAAGCGCATCGTACGCAACAAAACACGGCTCAGGAATGATGACTTCCTCACCTGGATTCACAATGGCGCGGATCGCAATATCCAAAGCCTGACTTGCCCCCACTGTCACAATCAGCTCATTATCTGGCGAGTAGCTAAGGTCAAACCTGTTGCTCAAAT > CP006881/3044286‑3044557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |