Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,203,656 T→C 7.5% W27R (TGG→CGG)  U712_16490 → Transcriptional regulatory protein CssR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,203,6560TC 7.5% 219.3 / 7.4 79W27R (TGG→CGG) U712_16490Transcriptional regulatory protein CssR
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base C (4/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

AAATTGCCTGCCGTATGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGAA  >  CP006881/3203519‑3203786
                                                                                                                                         |                                                                                                                                  
aaaTTGCCTGCCGTATGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTg                                                                                                                                   <  5:58972/139‑1 (MQ=255)
 aaTTGCCTGCCGTATGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTgg                                                                                                                                  <  1:318380/139‑1 (MQ=255)
 aaTTGCCTGCCGTATGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTgg                                                                                                                                  <  5:141075/139‑1 (MQ=255)
 aaTTGCCTGCCGTATGTTAAAAATAGTTTATAATGAAGTTTAAATGATGTGAAGAGCGTTGTCATACACCATTTATCTAGTTGAAGATGATGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGTGCTgg                                                                                                                                  <  1:46945/139‑1 (MQ=255)
               tGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCttt                                                                                                                    >  2:65802/1‑139 (MQ=255)
                    aaaaTAGTTTATAATGACGTTGAAAGTATGTGAAGAGCCTTTTCATACACCATTTATCTAGTTGAACATGACGATAACCTGAATGAACTGCTGACTAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGa                                                                                                               <  4:23544/139‑1 (MQ=255)
                    aaaCTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACg                                                                                                                >  6:253502/1‑138 (MQ=255)
                     aaCTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGa                                                                                                               >  5:203227/1‑138 (MQ=255)
                          gTTTATAATGCCGTGGAAAGGATGTGAAGCGCCTTGTCATACGCCAGTTATCTAGTTGAAGATGAGGATAACCTGAAGGCACTGCTGACGAAGTATTAAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGt                                                                                                          >  4:287525/1‑138 (MQ=255)
                          gTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGt                                                                                                          >  6:73333/1‑138 (MQ=255)
                          gTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGGATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTg                                                                                                         >  8:189186/1‑139 (MQ=255)
                                    aCGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGATGTATTTAGAGAATGAGGGCCGGAACATTACATCTTTTACGAAAGGTGaa                                                                                                       <  4:299854/131‑1 (MQ=255)
                                    aCGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGc                                                                                                  >  8:64088/1‑136 (MQ=255)
                                    aCGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGc                                                                                                  >  1:213037/1‑136 (MQ=255)
                                    aCGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCCGGAACATTACATCTTTTACGAAAGGTGaa                                                                                                       >  3:299854/1‑131 (MQ=255)
                                       ttGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGACCTGCTGACGAAGTATGTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCaga                                                                                              >  2:31741/1‑137 (MQ=255)
                                                tgtgAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGac                                                                                   >  5:242756/1‑139 (MQ=255)
                                                         ccTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTcc                                                                          >  3:336102/1‑139 (MQ=255)
                                                          cTTGTCATACACCATTTATCTTGTTGATGAGGAGGATAACCTGAATGAACTGCTGACTAAGTATTTAGAGAATGAGGGCTGTAAAATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGtctcac                                                                         <  2:100163/139‑3 (MQ=255)
                                                          cTTGTCATACACCATTAATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTccc                                                                         <  8:205743/139‑1 (MQ=255)
                                                             gTCATACACCATTTATATAGTTTAAGATGAGGATAACCTTAATGAACTGCTTAAGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACTAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCAc                                                                      <  4:37773/139‑1 (MQ=255)
                                                                aTACACCATTTATCTAGTTGAAGATGAGGATAACCTTAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCt                                                                    <  3:178142/138‑1 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGa                                                                                                               <  3:321344/92‑1 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGa                                                                                                               >  4:321344/1‑92 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCg                                                                               >  6:278724/1‑124 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCg                                                                               <  5:278724/124‑1 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATgg                                                                <  7:9718/139‑1 (MQ=255)
                                                                   cacCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCCGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATg                                                                 >  6:98419/1‑138 (MQ=255)
                                                                      cATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTAAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGAtt                                                             <  4:274644/139‑1 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           >  8:202422/1‑139 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           >  5:95595/1‑139 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           <  5:73333/139‑1 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           >  1:61015/1‑139 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           <  1:65802/139‑1 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           <  8:327011/139‑1 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           <  6:95595/139‑1 (MQ=255)
                                                                        tttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           >  3:37773/1‑139 (MQ=255)
                                                                         ttATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTACAGACTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATtct                                                           <  7:299686/138‑1 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAATGTGAATACGCCAGAAAGAAAATGACACCGTCTCCCCACATATCGATTCTCGATATCa                                                   >  2:111497/1‑139 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   >  2:327609/1‑139 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   <  7:64088/139‑1 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   >  2:99822/1‑139 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   >  7:159945/1‑139 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCCGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   >  8:227156/1‑139 (MQ=255)
                                                                                gTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTAGTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGAGTCTCGATATCa                                                   >  1:5722/1‑139 (MQ=255)
                                                                                   gAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCAtg                                                 <  7:11491/138‑1 (MQ=255)
                                                                                   gAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCAtg                                                 <  2:213037/138‑1 (MQ=255)
                                                                                   caaGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCAtg                                                 <  6:216547/137‑1 (MQ=255)
                                                                                      gATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCAtgctgc                                             >  5:331581/1‑139 (MQ=255)
                                                                                          aGGATAACCTGAATGAACTGCTGACTAAGAATATAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCATAAAGAAAATGACACCTTCTACCAACCTATGGATTCTAGATATAAtgatgcgag                                          >  2:120006/1‑135 (MQ=255)
                                                                                          aGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTAGCGATATCATGCTGACGGa                                         >  6:349725/1‑139 (MQ=255)
                                                                                              tAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATAcc                                     <  7:202422/139‑1 (MQ=255)
                                                                                              tAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATAcc                                     >  4:37671/1‑139 (MQ=255)
                                                                                              tAACCTGAATGAACGGCTGACGAAGAATTTATAGAATGAGGGCTGGACCATTCCATCTTTTCATAAAGGGGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACATATGGATTCTCTATAGCATGCTGCCGGATAcc                                     <  3:287525/139‑1 (MQ=255)
                                                                                                aCCTGAATTAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGGGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGa                                   <  6:331581/139‑1 (MQ=255)
                                                                                                aCCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCg                                    <  2:61015/138‑1 (MQ=255)
                                                                                                aCCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGa                                   <  6:242756/139‑1 (MQ=255)
                                                                                                aCCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGa                                   <  4:117090/139‑1 (MQ=255)
                                                                                                aCCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGa                                   <  7:145111/139‑1 (MQ=255)
                                                                                                aCCTGAAGGAACTGGTGACGAAGTTTTTAGTGAATGCGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGTTTCTCGATATCATGCTGCCGGATACCGa                                   <  6:203227/139‑1 (MQ=255)
                                                                                                 ccTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   <  8:242233/122‑1 (MQ=255)
                                                                                                 ccTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCa                                                   >  7:242233/1‑122 (MQ=255)
                                                                                                     aatgaaCTGCTGACGAAGTATATAGAGAATGAGGGCCGGAACATTACATCTTTAACGAAAGGAGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCt                              >  6:93152/1‑139 (MQ=255)
                                                                                                            tgctgACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCta                             <  5:104653/133‑1 (MQ=255)
                                                                                                            tgctgACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCta                             >  6:104653/1‑133 (MQ=255)
                                                                                                            tgctgACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACAt                        >  3:187080/1‑138 (MQ=255)
                                                                                                                    aaGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATtaataa                  >  4:116343/1‑136 (MQ=255)
                                                                                                                        aTTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGAGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGAAACCGACGGCTATACATTATTAAAAGAAAt           >  2:307958/1‑139 (MQ=255)
                                                                                                                         ttttGAGAATGAGGGCTGGAACATTACATCTTTTACGAACGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCTATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAAt           <  1:207117/138‑1 (MQ=255)
                                                                                                                              agaATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGacac                                                                                 <  6:105652/63‑1 (MQ=255)
                                                                                                                              agaATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGacac                                                                                 >  5:105652/1‑63 (MQ=255)
                                                                                                                                 aTGAGGGCTTGAACATTACATCTATTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGTTTCTCGATTTCATGCTGCCGGATAGCGACGGGTATACATTAATAAAAGAAATCAAGGCGaa  <  2:19256/139‑1 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGCCGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGa   >  6:100781/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGCCGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGa   >  6:317282/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGCCGGCTATACAGTAATAAAAGAAATCATGGCGa   >  1:158180/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGa   >  6:252790/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGa   >  8:270672/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGa   >  5:158228/1‑138 (MQ=255)
                                                                                                                                 aTGAGGGCCGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGaa  <  7:227156/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
AAATTGCCTGCCGTATGTTAAAACTAGTTTATAATGACGTTGAAAGGATGTGAAGAGCCTTGTCATACACCATTTATCTAGTTGAAGATGAGGATAACCTGAATGAACTGCTGACGAAGTATTTAGAGAATGAGGGCTGGAACATTACATCTTTTACGAAAGGTGAAGACGCCAGAAAGAAAATGACACCGTCTCCCCACCTATGGATTCTCGATATCATGCTGCCGGATACCGACGGCTATACATTAATAAAAGAAATCAAGGCGAA  >  CP006881/3203519‑3203786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑