BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,091,489	(T)_6→7	coding (1157/1326 nt)	U712_05390 ←	NTD biosynthesis operon protein ntdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,091,483	1	.	T	97.9%	480.3 / 1.5	140	coding (1163/1326 nt)	U712_05390	NTD biosynthesis operon protein ntdA
Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (60/77); total (61/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

TGAAACAGTCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCT  >  CP006881/1091348‑1091614

tGAAACAGTCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttt                                                                                                                                    >  8:407495/1‑139 (MQ=255)

        tCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttttt‑ttgcat                                                                                                                           >  4:253811/1‑134 (MQ=255)

        tCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttttt‑ttgca                                                                                                                            >  8:121902/1‑134 (MQ=255)

        tCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttttt‑ttgca                                                                                                                            >  7:87964/1‑134 (MQ=255)

        tCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttttt‑ttgca                                                                                                                            >  4:400314/1‑134 (MQ=255)

            tCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAATGGAGT‑TTTTT‑TGCa‑tat                                                                                                                         >  5:6980/1‑137 (MQ=255)

              cATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCCAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATgaga                                                                                                                     <  4:423384/139‑1 (MQ=255)

              cATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATgaga                                                                                                                     <  8:360933/139‑1 (MQ=255)

                ttACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGTCTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATgaga                                                                                                                     <  2:55048/137‑1 (MQ=255)

                  aCTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGt                                                                                                                 >  2:172063/1‑139 (MQ=255)

                  aCTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGAAGTACCTGACTATCAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑TTTTT‑TTTATTATGAGAAAg                                                                                                                  >  6:164339/1‑138 (MQ=255)

                   ctctGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTa                                                                                                                <  7:417261/139‑1 (MQ=255)

                   ctctGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTa                                                                                                                <  3:400314/139‑1 (MQ=255)

                   ctctGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTa                                                                                                                <  8:423227/139‑1 (MQ=255)

                   ctctGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTa                                                                                                                <  3:253811/139‑1 (MQ=255)

                      tGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAACGGAGT‑TTTTT‑TGCA‑GTTGAGATAGTAtcg                                                                                                             >  4:453856/1‑137 (MQ=255)

                      tGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTg                                                                                                             >  6:267283/1‑139 (MQ=255)

                      tGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAAGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑ttttt                                                                                                                                  >  8:326754/1‑119 (MQ=255)

                        tgttGTTCTTCTTAAGTAAATGACGGATATTAAGGCAAATGCAGTTCGTGTCTATGAGCCTTTTCCGTATGTACCTATTGGAGTCCTGGATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  4:98863/137‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TTCA‑TATGAGAAAGTATTGGa                                                                                                           >  5:332993/1‑139 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTAtt                                                                                                              <  6:405104/136‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  1:172063/139‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  4:343557/1‑139 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  6:466539/139‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  8:64982/1‑139 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  3:228574/1‑139 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  1:75375/139‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCAGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  7:388826/139‑1 (MQ=255)

                        tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGGCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTAtt                                                                                                              <  6:189915/136‑1 (MQ=255)

                        gcTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  4:92301/138‑1 (MQ=255)

                         cTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  3:30359/138‑1 (MQ=255)

                             ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGataata                                                                                                      >  8:256056/1‑139 (MQ=255)

                             ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  5:159102/134‑1 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTAtt                                                                                                              <  4:197891/126‑1 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTAtt                                                                                                              >  3:197891/1‑126 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  1:18203/129‑1 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACa                                                                                                  >  2:208724/1‑138 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACa                                                                                                  >  5:450745/1‑138 (MQ=255)

                                  ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGGCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGTATAATATACa                                                                                                  >  5:373037/1‑138 (MQ=255)

                                          aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  7:396355/1‑121 (MQ=255)

                                          aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                          >  8:65052/1‑138 (MQ=255)

                                          aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                          >  8:72363/1‑138 (MQ=255)

                                          aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                          >  8:185168/1‑138 (MQ=255)

                                          aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                          >  4:430980/1‑138 (MQ=255)

                                                  atatAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCaa                                                                                  >  3:250740/1‑138 (MQ=255)

                                                          gCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCat                                                                         <  5:430734/139‑1 (MQ=255)

                                                            aaaTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatt                                                                        >  3:311228/1‑138 (MQ=255)

                                                              aTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaa                                                                     >  1:131168/1‑139 (MQ=255)

                                                              aTGCAGTACCTGACTATGAGACTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaa                                                                     <  8:87964/139‑1 (MQ=255)

                                                                  aGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaattta                                                                 <  7:256056/139‑1 (MQ=255)

                                                                    tACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATgaga                                                                                                                     <  3:474668/85‑1 (MQ=255)

                                                                       cTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCAt                                                            <  4:250740/139‑1 (MQ=255)

                                                                       cTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCAt                                                            <  3:237675/139‑1 (MQ=255)

                                                                         gACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  3:369634/90‑1 (MQ=255)

                                                                         gACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  4:369634/1‑90 (MQ=255)

                                                                         gACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGa                                                          >  3:460623/1‑139 (MQ=255)

                                                                         gACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGa                                                          >  1:32592/1‑139 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           <  5:134005/85‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGa                                                                                                           >  6:134005/1‑85 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTTATGatat                                                       <  2:129198/137‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       <  2:370508/137‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       <  7:185168/137‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       >  6:427732/1‑137 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       <  5:427732/137‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                     <  2:131168/139‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                     >  5:215893/1‑139 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                     >  6:7435/1‑139 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                     <  7:121902/139‑1 (MQ=255)

                                                                              tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                     >  3:116930/1‑139 (MQ=255)

                                                                                aGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCa‑ta                                                                                                                          >  7:265916/1‑68 (MQ=255)

                                                                                aGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCa‑ta                                                                                                                          <  8:265916/68‑1 (MQ=255)

                                                                                aGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGata                                                                                                         >  3:139061/1‑85 (MQ=255)

                                                                                aGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGata                                                                                                         <  4:139061/85‑1 (MQ=255)

                                                                                 gCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGcc                                                  <  7:65052/139‑1 (MQ=255)

                                                                                 gCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGcc                                                  <  7:72363/139‑1 (MQ=255)

                                                                                   cTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCt                                                <  5:267283/139‑1 (MQ=255)

                                                                                         cGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                           <  7:284927/138‑1 (MQ=255)

                                                                                         cGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                           <  2:343819/138‑1 (MQ=255)

                                                                                         cGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                           <  7:64982/138‑1 (MQ=255)

                                                                                               aaCCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       >  8:406989/1‑120 (MQ=255)

                                                                                               aaCCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCt                                    <  5:426214/139‑1 (MQ=255)

                                                                                               aaCCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCt                                    <  7:416736/139‑1 (MQ=255)

                                                                                                     ttGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCt                               >  2:308870/1‑138 (MQ=255)

                                                                                                     ttGGAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCt                               >  6:133181/1‑138 (MQ=255)

                                                                                                       ggAGTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTa                             <  1:308870/138‑1 (MQ=255)

                                                                                                          gTCCTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAACTTCTGTTCTTACCTTAATAg                         >  2:151127/1‑139 (MQ=255)

                                                                                                             cTGCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGatat                                                       >  3:117816/1‑106 (MQ=255)

                                                                                                               gCATACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCATATCTACTGTTCTTACCTTAATAGGaac                     >  5:47014/1‑137 (MQ=255)

                                                                                                                  tACTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGa                 <  6:441246/139‑1 (MQ=255)

                                                                                                                   aCTTGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGAt                >  7:153502/1‑139 (MQ=255)

                                                                                                                    cTTGTACTGTACTAAAGGAG‑‑TTTTTATGCA‑TATGAGATAGTATTGGATTATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGGCATGTTATCTGCCCTTTCTTCTGTTCTTACCTTATTAGTAAACAGATg               <  6:99890/139‑1 (MQ=255)

                                                                                                                     ttGTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGc              >  6:31015/1‑139 (MQ=255)

                                                                                                                       gtacTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGATAAAGATTTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATTTCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGc              <  4:374987/134‑1 (MQ=255)

                                                                                                                       gTCCTGTACTAAAGGAGTTTTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGc              <  6:208316/138‑1 (MQ=255)

                                                                                                                       gTCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCa            >  5:396509/1‑139 (MQ=255)

                                                                                                                        tCCTGTACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCAt           <  8:453143/139‑1 (MQ=255)

                                                                                                                             tACTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACAt       >  1:64392/1‑138 (MQ=255)

                                                                                                                               cTAAAGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGAt    >  3:30540/1‑139 (MQ=255)

                                                                                                                               cTAAAGGAGT‑TTTTT‑TGAA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGAt    >  2:337613/1‑139 (MQ=255)

                                                                                                                                 aaaGGAGT‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCt  <  4:311228/139‑1 (MQ=255)

TGAAACAGTCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGAG‑‑TTTTT‑TGCA‑TATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCT  >  CP006881/1091348‑1091614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`