Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,289,100 G→T P184T (CCG→ACG)  U712_11585 ← putative oxidoreductase yqjQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,289,1000GT91.7% 95.3 / ‑0.6 37P184T (CCG→ACG) U712_11585putative oxidoreductase yqjQ
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (18/15);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCC  >  CP006881/2288965‑2289227
                                                                                                                                       |                                                                                                                               
tGATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGCCATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc                                                                                                                              >  7:61741/1‑139 (MQ=255)
 gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc                                                                                                                              <  2:70130/138‑1 (MQ=255)
  aTCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGccc                                                                                                                             <  7:355800/138‑1 (MQ=255)
  aTCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCg                                                                                                                            >  8:52816/1‑139 (MQ=255)
       ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCGTTATCAGCAATGGAAAAAAAGTCCGTCTTAATCTTGCCCGGGtt                                                                                                                        >  5:6409/1‑138 (MQ=255)
                cGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACtgttgt                                                                                                               <  4:322407/138‑1 (MQ=255)
                cGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACtgttgt                                                                                                               <  2:144257/138‑1 (MQ=255)
                          gCAGCGGTAATTTCAGCTGCCACGTCACAAGTAACAAGCTTCCAGCGGCCGACATTTTTGGCGTAGACCCCGCCTTAATCACCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGc                                                                                                    <  2:253062/139‑1 (MQ=255)
                                             ccACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTcc                                                                                   <  1:136555/137‑1 (MQ=255)
                                                     tcaGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCGTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAg                                                                         >  6:217906/1‑139 (MQ=255)
                                                             aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                 <  7:107688/139‑1 (MQ=255)
                                                             aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                 <  1:1173/139‑1 (MQ=255)
                                                              aGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATAGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCGCCAACCCCCAAGCGATTGAGt                                                                >  6:260992/1‑139 (MQ=255)
                                                                  tCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTATGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAAcc                                                            <  1:335039/139‑1 (MQ=255)
                                                                   ccAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAAcc                                                            >  3:433240/1‑138 (MQ=255)
                                                                    cAGCGGCCGACATTTTTGGCGTAGTACCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTACCGAAAGCACCATCCCCAAAGCGTTTGAGTAAcca                                                           >  6:392331/1‑137 (MQ=255)
                                                                     aGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTaa                                                         <  1:67035/139‑1 (MQ=255)
                                                                       cGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAATTCCGTCTGAATCGTGCCCGGGTTTACTGTTGTCCCATAAATTCCGGTGCCCGAAAGCTCCATACCCAAACCGTTTGAGTAACCTAaca                                                       >  6:291212/1‑139 (MQ=255)
                                                                          ccGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACgg                                                    >  6:326166/1‑139 (MQ=255)
                                                                                    tGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTgg                                           <  4:433240/138‑1 (MQ=255)
                                                                                        gtcgccccgGCCTTTATCACAAATGGAAAAAAAGTCGGTCTGAAGCGGGCCCGGGTTGACTGTTGTCACATAATTGCCGGTCCCCTAACGCTCCATCCGCAACGCGTTTGAGTACCCTAACACGGCATGTTTGGTcgcg                                      <  5:260992/134‑1 (MQ=255)
                                                                                        gTAGTCCCCGCCTTTATAAGAAATGGAAAAATAGTCCGTCTGAATCGTGCCCGGGTTGACTTTTGTCACATAAATGCCGGTTCCCCAAAGCTCCATCCGCAAAGCGTTTGATTAACATAACACGGCATGTTTGGTcgcg                                      <  4:31080/139‑1 (MQ=255)
                                                                                         tAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGAc                                                                                                                     <  2:409422/59‑1 (MQ=255)
                                                                                         tAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGAc                                                                                                                     >  1:409422/1‑59 (MQ=255)
                                                                                              cccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa                                 <  8:61741/138‑1 (MQ=255)
                                                                                                cGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTcgcg                                      >  5:204620/1‑131 (MQ=255)
                                                                                                cGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTcgcg                                      <  6:204620/131‑1 (MQ=255)
                                                                                                     ttATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGAATGTTGTAACATAAATGCCGGTTCCCGAAAGCTCCATCAGCAAAGCTTTTGATTAACCTAACACGGCATGTTTGGTAGCGGAATACatca                            >  1:268839/1‑133 (MQ=255)
                                                                                                      tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAACGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGa                        >  3:23482/1‑139 (MQ=255)
                                                                                                      tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGCGTAACCTAACACGGCATGGTTGGTCGCGGAATACCGGCTAgc                        >  4:112410/1‑138 (MQ=255)
                                                                                                      tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCCCAAAGCTTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGa                        >  3:305489/1‑139 (MQ=255)
                                                                                                             aaTGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                 >  2:4333/1‑91 (MQ=255)
                                                                                                             aaTGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                 <  1:4333/91‑1 (MQ=255)
                                                                                                                    aaaaaGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGa          >  4:266467/1‑139 (MQ=255)
                                                                                                                      aaaGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGAtt        >  3:213573/1‑139 (MQ=255)
                                                                                                                        cgTCGGTCTGAAAGGCGCCGGGGTTGACGGTTGTCACATAAATGCCGGTCCCGTAAAGCGCCATCCGAAAAGCGTTTGAGTAACATAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGAtttt      <  5:392331/138‑1 (MQ=255)
                                                                                                                            cGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTcccc  >  5:369579/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                               
TGATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCC  >  CP006881/2288965‑2289227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑