BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,214	C→T	intergenic (–/‑764)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,214	0	C	T	99.1%	26686.1 / 89.7	7628	intergenic (–/‑764)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (31/41); new base T (3050/4479); total (3104/4524)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7634 total aligned reads displayed.

AATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTA  >  pSIJ663_pHT315_araE_xy/2077‑2349

 aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  3:442910/139‑1 (MQ=255)

 aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  5:323733/139‑1 (MQ=255)

 aTCCGGGAAACATCTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  8:187953/139‑1 (MQ=255)

    cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGtctc                                                                                                                                    >  1:432005/1‑139 (MQ=255)

    cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGtctc                                                                                                                                    >  6:254787/1‑139 (MQ=255)

    cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       >  2:204147/1‑136 (MQ=255)

     gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGTTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTctct                                                                                                                                   >  7:166099/1‑139 (MQ=255)

      ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  8:445275/134‑1 (MQ=255)

      ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCtt                                                                                                                                  >  5:290458/1‑139 (MQ=255)

      ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCtt                                                                                                                                  >  1:2647/1‑139 (MQ=255)

      ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTGGTCTCtt                                                                                                                                  >  2:179268/1‑139 (MQ=255)

       gAAACATTTTTACATAGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACTACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTAttt                                                                                                                                 <  6:385229/139‑1 (MQ=255)

           cATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTatat                                                                                                                             <  3:74890/139‑1 (MQ=255)

                   cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattt                                                                                                                     <  6:180908/139‑1 (MQ=255)

                   cATCGGTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattt                                                                                                                     <  2:240177/139‑1 (MQ=255)

                     tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                   <  8:242373/139‑1 (MQ=255)

                      cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattttat                                                                                                                  >  3:142139/1‑139 (MQ=255)

                      cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                   >  4:315489/1‑138 (MQ=255)

                      cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                   >  7:254787/1‑138 (MQ=255)

                      cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                   >  8:216218/1‑138 (MQ=255)

                        ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCCCATCTTGTCTCTTTATATCTTTATTTTATCa                                                                                                                >  3:474363/1‑139 (MQ=255)

                           gAAGTGCAAGTCTAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATaa                                                                                                             <  2:449925/139‑1 (MQ=255)

                           gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTAGAATC‑TTTTTTTTATTATCCGTAAAATTTCTACCTCATCTTGTCTCTTTATATCTTTATTTTATCATa                                                                                                              <  7:392782/138‑1 (MQ=255)

                              gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                          <  1:137129/139‑1 (MQ=255)

                              gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                          <  6:99216/139‑1 (MQ=255)

                               tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                         <  4:402841/139‑1 (MQ=255)

                               tGCAAATGAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                         <  4:371851/139‑1 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       >  8:115498/1‑107 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  4:344443/107‑1 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCTTATAGGAGt                                                                                                       >  4:466587/1‑139 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                        >  4:142653/1‑138 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                        >  7:198607/1‑138 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                        >  5:185468/1‑138 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                        >  8:379750/1‑138 (MQ=255)

                                 caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATATTGTCTCTTTATATCTTTATTTTATCATAAagtagt                                                                                                       >  7:428747/1‑139 (MQ=255)

                                    gtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAtt                                                                                                    >  5:71240/1‑139 (MQ=255)

                                      caagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAttt                                                                                                   >  8:277327/1‑138 (MQ=255)

                                         gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  3:243884/99‑1 (MQ=255)

                                                 tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  5:396561/138‑1 (MQ=255)

                                                 tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  5:426955/138‑1 (MQ=255)

                                                 tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  8:62016/138‑1 (MQ=255)

                                                 tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  3:377/138‑1 (MQ=255)

                                                 tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  2:342274/138‑1 (MQ=255)

                                                  aTCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTAATCTTGTCTCTTTATATCtttatttta                                                                                                                   >  2:148153/1‑110 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATAATAAAGGAGTATTTGAACCGTcgcgcg                                                                                      >  5:25982/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       <  6:108578/89‑1 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                       >  5:108578/1‑89 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  2:6192/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  5:365304/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  3:215206/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  7:75111/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  3:311983/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  7:461526/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  1:101336/1‑139 (MQ=255)

                                                   tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTATCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAACGTCGCGCgg                                                                                     >  7:28773/1‑139 (MQ=255)

                                                    cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTta                                                                                                                                >  1:368279/1‑95 (MQ=255)

                                                    cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                     >  3:100027/1‑138 (MQ=255)

                                                     tataATGATTTGTGGAAGTTTTTCCACAAAAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTGCTACCTCATCTTGTCTCTTTATATCTTTATTTTCTAATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   <  6:31929/139‑1 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   >  3:348238/1‑137 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   <  1:461302/137‑1 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   >  1:73375/1‑137 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                  <  4:211104/138‑1 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                  <  1:30520/138‑1 (MQ=255)

                                                       taATGATTTGTGGAAGTTTTGCCACAACAGTTGG‑TTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   <  6:214240/136‑1 (MQ=255)

                                                          tGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                   <  6:61469/134‑1 (MQ=255)

                                                            aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt                                                                            <  2:411332/139‑1 (MQ=255)

                                                            aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                             >  5:227251/1‑138 (MQ=255)

                                                            aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                             <  7:129886/138‑1 (MQ=255)

                                                            aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TAATTTTTATTAGCCGTCAAATTTCTCACTCATCTTGTCTCATTATATCTTTATTTTATCATAAAGTAGAAAATGAACCGTCGCGCGGGACAGCtt                                                                             >  4:177723/1‑138 (MQ=255)

                                                             tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                  <  1:22847/132‑1 (MQ=255)

                                                               tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                        <  6:289917/124‑1 (MQ=255)

                                                                 tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                  >  7:362681/1‑128 (MQ=255)

                                                                 tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                        <  1:195132/138‑1 (MQ=255)

                                                                 tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                       <  3:408906/139‑1 (MQ=255)

                                                                 tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                       >  4:28174/1‑139 (MQ=255)

                                                                 tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                       >  1:164829/1‑139 (MQ=255)

                                                                     aGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                                   <  7:330015/139‑1 (MQ=255)

                                                                     aGTTTTGCCACAACAGTAGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATATCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                                   <  1:394316/139‑1 (MQ=255)

                                                                      gTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAGGGAGTATTTGAACCGTCGCGCGGGa                                                                                   <  6:449315/122‑1 (MQ=255)

                                                                         ttGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTATTTATATCGTTATTTTATCATAAAGGAGTATATGAACCGTCGCGCGGGACAGGTTTATGATAGGGatat                                                               >  2:275970/1‑139 (MQ=255)

                                                                             cacaACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                           <  2:376484/139‑1 (MQ=255)

                                                                                      tGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat                                                  >  4:245763/1‑139 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATATAGGAGTATTTGAACCGTCGCGCGGGACAGGGTTATGATAGGGATATTTTATTGAATATTTGa                                               <  4:107417/139‑1 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGTGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               <  8:351486/139‑1 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               <  1:333072/139‑1 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               <  4:435116/139‑1 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               <  4:76129/139‑1 (MQ=255)

                                                                                         tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               <  6:414097/139‑1 (MQ=255)

                                                                                           tatataCAATC‑CTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTAATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGTGATATTTTATTGAATAATTGAt                                              <  5:460544/138‑1 (MQ=255)

                                                                                                  aaTC‑TTTTTTTTATTAGACGTAAAATTTCTCCCTCATCTTGTCTGTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTGTATGATAGGGAGATTTTATTGAATAATTGATGGTATAAg                                      >  2:111100/1‑139 (MQ=255)

                                                                                                   aTC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                     <  3:281110/139‑1 (MQ=255)

                                                                                                   aTC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                     <  8:413271/139‑1 (MQ=255)

                                                                                                         ttttttATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATGTTTATTTTATCATAAAGGAGTTTTTGAACCGGCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGTACtt                                >  6:352857/1‑139 (MQ=255)

                                                                                                         ttttttATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACtt                                >  8:318657/1‑139 (MQ=255)

                                                                                                         ttttttATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACt                                 <  8:14772/138‑1 (MQ=255)

                                                                                                                 taGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaata                                                    >  2:311638/1‑111 (MQ=255)

                                                                                                                  aGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCtt                        <  5:145174/138‑1 (MQ=255)

                                                                                                                            tttCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTTATGGTATAAGGGACTTTAATGCTTGGAAAGTgggt             <  3:177855/139‑2 (MQ=255)

                                                                                                                                 cccTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                               >  7:228885/1‑100 (MQ=255)

                                                                                                                                  ccTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTAt        <  6:145025/138‑1 (MQ=255)

AATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTA  >  pSIJ663_pHT315_araE_xy/2077‑2349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`