BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,091,483:1	+T	100%	coding (1163/1326 nt)	U712_05390 ←	NTD biosynthesis operon protein ntdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,091,483	1	.	T	97.4%	389.6 / 2.0	116	coding (1163/1326 nt)	U712_05390	NTD biosynthesis operon protein ntdA
Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (65/48); total (67/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 121 total aligned reads displayed.

TCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑G‑T‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCTT  >  CP006881/1091356‑1091615

tCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAAATGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑G‑t‑ttt‑tttgca                                                                                                                            >  5:73777/1‑134 (MQ=255)

   cTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCatat                                                                                                                          >  3:399789/1‑138 (MQ=255)

      cATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATgaga                                                                                                                      <  5:185632/139‑1 (MQ=255)

           ctctGTCTTGTTCTTCTAAAGTAAATGACTGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTa                                                                                                                 <  7:123862/139‑1 (MQ=255)

           ctatGTCTTGTTCTTCTAAAGTAAATGAATGATATAAATGCAAATGCAGTACCTGACTATGTTCCTTTTCCGTATGAAACAATTGTAGTCCTGCATACTTGTCCTGTACTATAGTA‑GTT‑TTT‑TTGCATATGAGAAAGTa                                                                                                                 <  5:42718/136‑1 (MQ=255)

              tGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCATGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTg                                                                                                              >  1:306904/1‑139 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCata                                                                                                                           >  5:112462/1‑124 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCata                                                                                                                           <  6:112462/124‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGATAAAGTATTGGa                                                                                                            >  6:5564/1‑139 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGGAAGTATTGGa                                                                                                            <  1:96103/139‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTAtt                                                                                                               <  4:305463/136‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  2:306904/139‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  4:399789/139‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  2:107470/139‑1 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            >  1:107470/1‑139 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            >  7:269594/1‑139 (MQ=255)

                tCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGAATATGAGCCTTTTGCGTATGAACCAATTTGAGTCCTGCATACTTGTACTGTACTAAAGGT‑GTT‑TTT‑TTGCATATGAGAATGTATTGGa                                                                                                            <  7:380135/139‑1 (MQ=255)

                   tGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTACTGCATACTTGTACTGTACTAAAGGA‑GTT‑TTT‑TTGCTTATTAGAAAGTATTGGataa                                                                                                         >  6:42718/1‑139 (MQ=255)

                     ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATgaga                                                                                                                      >  6:95865/1‑124 (MQ=255)

                     ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATgaga                                                                                                                      <  5:95865/124‑1 (MQ=255)

                     ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  3:136508/134‑1 (MQ=255)

                     ttcttcTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTCGataat                                                                                                        >  5:276935/1‑138 (MQ=255)

                     ttcttcTAAAGTAAATGACGGAGATAAAGGCAAATGCAGTACCTGACTATGAGGCTTTTCCGTAAGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATAGGagaata                                                                                                       >  2:59490/1‑139 (MQ=255)

                        ttctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTACTGGATAAtata                                                                                                     >  6:11212/1‑138 (MQ=255)

                          ctAAATTAAATGTCTGATATAAAGGGAAATGCAGTACCTGACTATGAGCCTTTTCCTTATGAACCAATTGGAGTCCTGCATACTTGTCCTTTACTAAATGA‑GTT‑TTT‑TTGCATATGAGAAAGTTTTGGATATtata                                                                                                     <  2:320406/136‑1 (MQ=255)

                          ctAAAGTAAATGACGGATATAAATGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTa                                                                                                                 >  2:233757/1‑124 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTTACTATGAGCCTTTTCCGTATGAACCAATTCGAGTCCTGAATACTTCTCCTGTACTAAAGGA‑G‑TATTA‑TTGCAGATGAGAAAGTATTGGATAAGATACAt                                                                                                  >  8:380135/1‑139 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTa                                                                                                                 <  1:233757/124‑1 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  6:282924/129‑1 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            >  5:282924/1‑129 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACAt                                                                                                  >  7:160765/1‑139 (MQ=255)

                          ctAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑G‑t‑ttt‑t                                                                                                                                 <  5:36865/107‑1 (MQ=255)

                          ctAAAGTAAATGAAGTATATAAAGGCAAATGCAGTACCTGACTATGAGCATTTTCCGTATGAACCAATTGGAGTCATGCATACTTGACCTGTACTAAAGGA‑GTT‑TTT‑TTGGATATGAGAAAGTAATGGATAAtataaa                                                                                                   >  6:117410/1‑136 (MQ=255)

                                  aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                           >  1:314079/1‑138 (MQ=255)

                                  aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                           >  1:283999/1‑138 (MQ=255)

                                  aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                           >  8:326727/1‑138 (MQ=255)

                                  aaTGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTg                                                                                           >  4:245600/1‑138 (MQ=255)

                                        ggATATAAAGGAAAATGCAGTAACTGACTATGAGGCTTTTCCGTATGAACCATTTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCa                                                                                    <  6:276935/139‑1 (MQ=255)

                                          atatAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTAGTGGATAATATACATCTGTTTGAACACCaa                                                                                   >  7:6802/1‑138 (MQ=255)

                                                    aaaTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑G‑t‑ttt‑tttgca                                                                                                                            >  7:8095/1‑82 (MQ=255)

                                                     aaTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCattta                                                                       >  2:274586/1‑139 (MQ=255)

                                                     aaTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCattt                                                                        >  1:40039/1‑138 (MQ=255)

                                                     aaTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCattt                                                                        >  5:308103/1‑138 (MQ=255)

                                                      aTGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaa                                                                      >  1:378787/1‑139 (MQ=255)

                                                         cAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaatt                                                                    <  1:52682/138‑1 (MQ=255)

                                                         cAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTACTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaatt                                                                    <  5:11212/138‑1 (MQ=255)

                                                          aGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaattta                                                                  <  8:5908/139‑1 (MQ=255)

                                                              ccTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTTGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCa                                                              <  2:314079/139‑1 (MQ=255)

                                                               cTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCAt                                                             <  7:326727/139‑1 (MQ=255)

                                                                 gACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGa                                                           >  5:4510/1‑139 (MQ=255)

                                                                  aCTATGAGCCTTTTCCGTATGAACAAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTAtt                                                                                                               <  6:125322/86‑1 (MQ=255)

                                                                    tATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTTCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACAACAAAATATTCATTTAATTTAGTCATGatat                                                        <  5:173228/139‑1 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            >  3:250509/1‑85 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATc                                                                                                 >  1:287742/1‑96 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATc                                                                                                 <  2:287742/96‑1 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaatt                                                                    >  7:174434/1‑125 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCatttaatt                                                                    <  8:174434/125‑1 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                      >  7:34088/1‑139 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                      >  4:68424/1‑139 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCt                                                      <  8:269594/139‑1 (MQ=255)

                                                                      tGAGCCTTTTCCGTATGAACCAATTGGAGTCATGCATACTTGTCCTGTACTAAAGGATT‑T‑TTT‑TTGCATATGAGAAAGTATTGGa                                                                                                            <  4:250509/85‑1 (MQ=255)

                                                                         gCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGcc                                                   <  1:309752/139‑1 (MQ=255)

                                                                           cTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCt                                                 <  6:4510/139‑1 (MQ=255)

                                                                           cTTTTACGTATGAACCAATTGGAGTCCAGCATACTTGTACTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCt                                                 <  5:250284/139‑1 (MQ=255)

                                                                            ttttCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAacac                                                                                      <  4:217194/101‑1 (MQ=255)

                                                                            ttttCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAacac                                                                                      >  3:217194/1‑101 (MQ=255)

                                                                            ttttCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGcc                                                   >  7:191871/1‑136 (MQ=255)

                                                                                 cGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                            <  3:245600/138‑1 (MQ=255)

                                                                                  gTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtcttct                                          >  4:96723/1‑139 (MQ=255)

                                                                                  gTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtcttct                                          >  8:334272/1‑139 (MQ=255)

                                                                                  gTATGAACCAATTGGAGTCCTGCATACTTGTACTGAACTAAAGGA‑C‑T‑TTTATTGCAAATGAGAAAGTATTGGATAATATACATCTGTTTTAACACCAAAATCTTCATTTAATTAAGACATGATATCTGCCCTAtcttct                                          >  6:250284/1‑139 (MQ=255)

                                                                                       aaCCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGtagt                                     >  7:243700/1‑136 (MQ=255)

                                                                                        aCCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATATAATTTAGTCATGATATCTGCCCTATCTTCTGTTCt                                     >  1:336455/1‑138 (MQ=255)

                                                                                             ttGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCt                                >  8:44118/1‑138 (MQ=255)

                                                                                                gAGTCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTaa                             <  8:34088/138‑1 (MQ=255)

                                                                                                   tCCTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAg                          >  3:158856/1‑138 (MQ=255)

                                                                                                     cTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                            >  6:73489/1‑118 (MQ=255)

                                                                                                     cTGCATACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                            <  5:73489/118‑1 (MQ=255)

                                                                                                          tACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCATAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGa                  <  2:336455/139‑1 (MQ=255)

                                                                                                          tACTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGa                  <  2:328120/139‑1 (MQ=255)

                                                                                                            cTTGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGAt                 <  6:384557/138‑1 (MQ=255)

                                                                                                             ttGTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGc               >  5:31390/1‑139 (MQ=255)

                                                                                                               gTCCTGTACTATAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGcc              <  8:60201/138‑1 (MQ=255)

                                                                                                               gTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTaa                             >  1:95871/1‑123 (MQ=255)

                                                                                                               gTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTaa                             <  2:95871/123‑1 (MQ=255)

                                                                                                               gTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGcc              >  7:62987/1‑138 (MQ=255)

                                                                                                               gTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGc               <  3:96723/137‑1 (MQ=255)

                                                                                                               gTCCTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCa             >  3:361172/1‑139 (MQ=255)

                                                                                                                  cTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTTAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTATGTTCTTACCTTAATAGGAAAAAGATGCCATa           >  8:205937/1‑138 (MQ=255)

                                                                                                                  cTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATc                                                                               >  1:210584/1‑70 (MQ=255)

                                                                                                                  cTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATc                                                                               <  2:210584/70‑1 (MQ=255)

                                                                                                                  cTGTACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATa           >  2:284627/1‑138 (MQ=255)

                                                                                                                     tACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACAt        >  1:1767/1‑138 (MQ=255)

                                                                                                                     tACTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACAt        >  6:202924/1‑138 (MQ=255)

                                                                                                                       cTAAAGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGAt     >  5:15378/1‑139 (MQ=255)

                                                                                                                          aaGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                            >  2:36017/1‑97 (MQ=255)

                                                                                                                          aaGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTAtctt                                            <  1:36017/97‑1 (MQ=255)

                                                                                                                          aaGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCtt  >  5:18548/1‑139 (MQ=255)

                                                                                                                          aaGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCt   <  1:261276/138‑1 (MQ=255)

                                                                                                                           aGGA‑GTT‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCGTTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCtt  >  6:25416/1‑138 (MQ=255)

TCCCTCCATTACTCTGTCTTGTTCTTCTAAAGTAAATGACGGATATAAAGGCAAATGCAGTACCTGACTATGAGCCTTTTCCGTATGAACCAATTGGAGTCCTGCATACTTGTCCTGTACTAAAGGA‑G‑T‑TTT‑TTGCATATGAGAAAGTATTGGATAATATACATCTGTTTGAACACCAAAATCTTCATTTAATTTAGTCATGATATCTGCCCTATCTTCTGTTCTTACCTTAATAGGAAACAGATGCCATACATGATCTT  >  CP006881/1091356‑1091615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`