| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 511,836 | A→C | 13.9% | E55D (GAA→GAC) | U712_02450 → | Protein sprT‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 511,836 | 0 | A | C | 13.9% | 96.6 / 2.1 | 52 | E55D (GAA→GAC) | U712_02450 | Protein sprT‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (27/17); new base C (2/6); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAGAGGATATTTCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAACGCGCCGAGGT > CP006881/511698‑511969 | gACAGAGGATATTTCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGaa > 2:228751/1‑139 (MQ=255) aacgagTATATTTATAAAAAATATTTAAAAAAGCCGTGCGCCCAGCAGGGGTTTTTAAATGACCGGAATAAAACCACGGGCGGCAGGTACCTGTTAACCTCCCATAACT‑TTTATTTCAACATAAAATATTTGATTGaa < 4:235133/135‑1 (MQ=255) cAGAGGATTTTTCTCAAACATATTTTAAAACGCCGTTCCGCCATCTGGCGCTTTTTAATGACCGGATTAAAACCAGGGGCGGCAGGTACCGGTTAACCTCCAATAACA‑TTGAATTTAACAGAAAATATTTCATTCAaca < 8:14645/139‑1 (MQ=255) agGATATTTCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACgg > 8:105426/1‑139 (MQ=255) gATATTTCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACgg > 1:84906/1‑137 (MQ=255) ttatttattAAACATTTTTTAAAAAGCCGTTCCGCCATCCGGCGCTTTTTATTCACCGGCTTAAAACCACTGGCGGCAGGTACCTGTAAACCTCCAATAACA‑TTGAATTCAACATAAAATATTTGATTGACCACGGCa < 2:61601/137‑1 (MQ=255) tttCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAgg > 6:195987/1‑137 (MQ=255) tCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGggag > 1:239204/1‑138 (MQ=255) aaaCATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACt > 1:111018/1‑139 (MQ=255) aaaCATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACt > 6:222085/1‑139 (MQ=255) aCATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCCCTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGa > 2:183825/1‑139 (MQ=255) tatTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTTACCTCCCATAACA‑TAGAATTGAACAGCAAATATTTGATTGACCACGGCAGGGAGGAACTGATTg > 7:143266/1‑139 (MQ=255) atTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTgg > 2:290302/1‑139 (MQ=255) taaaaacGCCGTTCCGCCTTCCGGCGTTTTTTATTGACCGGATAAAACCCTGTGGCGGGTGTTAGCTGTAAACCCCCAAAAACT‑TTCAATTCAACATAAAATTTTTTATTCACCACGGCAGGGAGGAACTGATTGGca < 4:345028/136‑1 (MQ=255) aaacGCCGTTCCCCAACCAGGCTTTTTTTATTGACCGGCAAAAAAACACTGGCGGCCGGTTCCTGTTAACCTCCCAAAACA‑TTTAATTGAAAATAAAATTTTTTATTTACCACGGCAGGGAGGAACTGATTGGcatcat < 4:316689/139‑1 (MQ=255) cGTACCGCCACCAGGCGAGTTTTAATGACCGGATTAAACCCCCGGGCGGCAGGCACCTGTAAACCTCCCATACCT‑TTGAATGGAACAGAAAATATTTGATTGACCACGGCAGGGAGGAACTGATTGGCATCATCAAGCa < 3:319585/139‑1 (MQ=255) ttCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACg > 3:205673/1‑139 (MQ=255) ttCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACg > 3:210892/1‑139 (MQ=255) ccATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGaa < 6:360684/97‑1 (MQ=255) ccATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGaa > 5:360684/1‑97 (MQ=255) ccATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCAGAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGctc > 8:367045/1‑139 (MQ=255) tCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGc > 8:301889/1‑139 (MQ=255) cttattAAAACCAGGGGCGGGAGTTACGTTTTAACAT‑CCAAAACATTTTATTTGAAAATAAAATATTTTATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTg < 4:210892/135‑1 (MQ=255) cgtcTTAATCCCAGTGGCCGGAGGTAGCTTTTAACACCCAAAAATA‑TTTATTTGAACAAAAAATATTTCATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTg < 4:205673/136‑1 (MQ=255) aaaaCCACTGGCGGCAGGTACCTGTAAACCTCCAATAACA‑TTTAATTGAACATAAAATATTTGATTTAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaa < 7:105426/139‑1 (MQ=255) aacccccTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTCAACATAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaaa < 2:289078/133‑1 (MQ=255) aaaCCCCGGGCGGCAGGTACCTTTAAACCTCCAATAACA‑TTTAATTTAAAATAAAATTTTTGATTCAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaaa < 1:228751/139‑1 (MQ=255) aaaCCACTGGCGGCAGGTACGTGTTAACCTCCCATAACT‑TTGAATTGAACAGAAAATATTTGATTTAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaaa < 4:86914/139‑1 (MQ=255) cTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAATCACGAGCTCTGCAATTATCATCTTCATCTTGAAGGAAAAGTGTa > 3:269068/1‑139 (MQ=255) cTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTa > 1:157147/1‑139 (MQ=255) cTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGCGAGGAAATCATTGGCATCATCAAGCACGAGATCTGCCATAATCATCTACATCTGGAAGGAAAAGGGTa > 2:299460/1‑139 (MQ=255) cTGGCGGCAGGTACCTGTTAACCTCCCAAAACA‑TAGAATTGAACAGAAAATATTTGATTGAACACGGCAGTGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGt > 2:20560/1‑138 (MQ=255) gcggcAGGTACCTGTTAACCTCCCATAACT‑TTGAATTGAACATAAAATATTTTATTTAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACa < 7:299950/138‑1 (MQ=255) gctgttACCTGTTTACAACCAAAAACT‑TTTATTTTAAAAGAAAAATTTTTAATAACCTCGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGca < 7:353111/134‑1 (MQ=255) gcAGGAACCTGTAAACCACCCATAACA‑TTCAATTGAAAACAAAATTTTTGATTCAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGca < 4:333501/139‑1 (MQ=255) aGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGca > 5:58647/1‑137 (MQ=255) tACCTTTTAACCAACCAAAAAA‑TTAAATATAAAATAAAATATTTTAAATAACAAGGCAGGGAGGAACTGAGTGGCATCATCCAGCACGAGCTCAGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCacag < 1:346408/137‑1 (MQ=255) ccTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCacagaga > 3:279874/1‑138 (MQ=255) ttAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCCTCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACACGCacagagaca > 5:268190/1‑136 (MQ=255) ccccAAAAAA‑TTTAAATCAACACAAAATATTTCATGCACCACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTa < 2:262465/138‑1 (MQ=255) cccATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCAc > 8:380628/1‑74 (MQ=255) cccATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCAc < 7:380628/74‑1 (MQ=255) cccAAAACA‑TTGAATTCAAAACAAAATATTTGATTCAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAg < 1:183825/139‑1 (MQ=255) cATAACA‑TTGAATTGAACATAAAATTTTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGaa < 8:233229/139‑1 (MQ=255) cATAACA‑TTGAATTCAACATAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGaa < 4:133440/139‑1 (MQ=255) tgaattgaaCAGAAAATATTTGATTGACCACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTg > 6:61184/1‑139 (MQ=255) gaattgaaCAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCAc < 2:272299/63‑1 (MQ=255) gaattgaaCAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCAc > 1:272299/1‑63 (MQ=255) attgaaCAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTgcag < 7:301889/139‑1 (MQ=255) aaCAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaa > 4:139540/1‑91 (MQ=255) aaCAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGaa < 3:139540/91‑1 (MQ=255) aaaaTATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAAc > 4:273854/1‑139 (MQ=255) aaaaTATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGAACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAAc > 7:247474/1‑139 (MQ=255) attgaACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTgcag > 7:253949/1‑120 (MQ=255) attgaACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTgcag < 8:253949/120‑1 (MQ=255) attgaACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAACGCGCCGAGGt > 1:169663/1‑139 (MQ=255) attgaACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAACGCGCCGAGGt > 6:153402/1‑139 (MQ=255) | GACAGAGGATATTTCTGAAACATATTTTAAAAAGCCGTTCCGCCATCAGGCGCTTTTTAATGACCGGCTTAAAACCACTGGCGGCAGGTACCTGTTAACCTCCCATAACA‑TTGAATTGAACAGAAAATATTTGATTGAACACGGCAGGGAGGAACTGATTGGCATCATCAAGCACGAGCTCTGCCATTATCATCTTCATCTTGAAGGAAAAGGGTACAAGCACAGAGACAGAGATTTTAGAATGCTATTGCAGCAGGTCAACGCGCCGAGGT > CP006881/511698‑511969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |