Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 558,709 T→C 5.6% P109P (CCA→CCG)  U712_02720 ← putative transporter ydfM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881558,7090TC 5.6% 215.7 / 3.5 73P109P (CCA→CCG) U712_02720putative transporter ydfM
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base C (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

GCCTTTATGATCGTACGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGTTT  >  CP006881/558571‑558843
                                                                                                                                          |                                                                                                                                      
gCCTTTATGATCGTACGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCtt                                                                                                                                        <  6:322475/139‑1 (MQ=255)
            gTACGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACa                                                                                                                            <  7:241528/139‑1 (MQ=255)
            gTACGCAATCGCTATAATCGCTTTGCTGTTCCATTTATTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACa                                                                                                                            <  7:350422/139‑1 (MQ=255)
             tACGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACa                                                                                                                            <  6:262703/138‑1 (MQ=255)
               cGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACaaa                                                                                                                          <  1:123487/138‑1 (MQ=255)
                    tCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGa                                                                                                                       >  6:56036/1‑136 (MQ=255)
                    tCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATAGGGGAACGCTTGGCCCTTTAACAAAGGa                                                                                                                       >  7:277811/1‑136 (MQ=255)
                       cTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAAt                                                                                                                 <  4:239481/139‑1 (MQ=255)
                       cTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAAt                                                                                                                 <  8:166132/139‑1 (MQ=255)
                                cTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTAt                                                                                                        >  8:340769/1‑139 (MQ=255)
                                cTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTAt                                                                                                        >  1:400699/1‑139 (MQ=255)
                                 tttGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  7:340769/139‑1 (MQ=255)
                                  ttGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTAt                                                                                                        >  3:333686/1‑137 (MQ=255)
                                  ttGCTGTTCCAGTTTGGCCCTTGCTTTATAGAATAAGGATATAGGATTGCCTTAGCCCCGTCCGATATCAGAGCCGCAGACCCTGCGCGGTAGTGGGGAACGCTTGGCCCGTTACCAAGGGACCGAATCGCTTGTATGa                                                                                                      >  6:250592/1‑139 (MQ=255)
                                   tGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  1:12609/137‑1 (MQ=255)
                                   tGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  5:56036/137‑1 (MQ=255)
                                   tGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  5:326504/137‑1 (MQ=255)
                                     cTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAgg                                                                                                    <  8:369241/138‑1 (MQ=255)
                                                    ccTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACg                                                                                     <  2:400699/138‑1 (MQ=255)
                                                    ccTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTGGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACg                                                                                     <  1:312353/138‑1 (MQ=255)
                                                     cTTGCTTTATAGAATAACGATCTAAGATCTCCTTAGCCACGTACGACATCAGAGCCGCAAAGAATGCGCTGTATTGGGGAACGCGTGGCCCTTTAACAAAGGACAGACTCGCTTCTATGAGGATATAAACGGATACGAt                                                                                   <  5:250592/139‑1 (MQ=255)
                                                              taGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGAt                                                                          <  5:169027/139‑1 (MQ=255)
                                                                gAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATa                                                                         <  5:7363/138‑1 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTTTGAGGATATAAACGGATACGATCACTAAGATAATTc                                                                     <  8:277811/139‑1 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGTATACGATCACTAAGATAATTc                                                                     >  7:23586/1‑139 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTc                                                                     >  3:335221/1‑139 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTc                                                                     >  5:244446/1‑139 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTc                                                                     >  5:11533/1‑139 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTc                                                                     >  7:100424/1‑139 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTCGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       >  3:165016/1‑105 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTCGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       >  3:170398/1‑105 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTCGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  4:170398/105‑1 (MQ=255)
                                                                   tAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTCGGCCCTTTAACAAAGGACAGAATCGCTTCTATg                                                                                                       <  4:165016/105‑1 (MQ=255)
                                                                      cGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTACCAAAGGACAGAATCGCTTATATTAGGATATAAACGGATACGAACACGAAGATAATTGCt                                                                   >  2:99360/1‑138 (MQ=255)
                                                                        atatAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCATAc                                                                 >  7:154804/1‑138 (MQ=255)
                                                                           taAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATc                                                             >  5:11558/1‑139 (MQ=255)
                                                                            aaGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCg                                                            <  6:11533/139‑1 (MQ=255)
                                                                             aGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCg                                                            <  6:11558/138‑1 (MQ=255)
                                                                                tttCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCt                                                         <  4:335221/138‑1 (MQ=255)
                                                                                  tCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCa                                                       <  8:100424/138‑1 (MQ=255)
                                                                                  tCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGAGCTGTATTGGGGACCGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTTAGATTATTCCTACAATCGCCTCAc                                                      >  1:111096/1‑139 (MQ=255)
                                                                                   ccTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATTTTAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCAc                                                      >  6:214106/1‑138 (MQ=255)
                                                                                   ccTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCAc                                                      >  4:196703/1‑138 (MQ=255)
                                                                                              tACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACg                                                                                     >  4:124063/1‑96 (MQ=255)
                                                                                              tACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACg                                                                                     <  3:124063/96‑1 (MQ=255)
                                                                                                     tCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTcc                                   <  3:196703/139‑1 (MQ=255)
                                                                                                         agCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGa                               >  7:221913/1‑139 (MQ=255)
                                                                                                              cAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCaa                           >  3:78534/1‑138 (MQ=255)
                                                                                                                 gaaaTGCGGTGTTTTGGTTAACGCTGGGACCTTTAAAATATGACAGAATCGCTTCTTTTCGTATATAAACGTATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAgg                       <  8:331838/137‑1 (MQ=255)
                                                                                                                 aCAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGAAAGCATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAgg                       <  2:111096/139‑1 (MQ=255)
                                                                                                                  cAATGCGCTGTTTTGGGGTCCGCTTGGCCCTTTAACAAAGTACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGa                      <  2:81098/139‑1 (MQ=255)
                                                                                                                  cAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGa                      <  6:244446/139‑1 (MQ=255)
                                                                                                                     tGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATgg                   >  2:216593/1‑139 (MQ=255)
                                                                                                                            tATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCt                                                         <  6:4641/94‑1 (MQ=255)
                                                                                                                            tATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCt                                                         >  5:4641/1‑94 (MQ=255)
                                                                                                                             aTTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTAGATGAGGATATAAAAGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGaa            >  7:331838/1‑137 (MQ=255)
                                                                                                                                 gggAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCaa                           <  5:394153/119‑1 (MQ=255)
                                                                                                                                 gggAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCaa                           >  6:394153/1‑119 (MQ=255)
                                                                                                                                    aaCGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCATTTCCGTGACCAAAATGATGGTCTTGATAGGGCGGt    >  6:117636/1‑139 (MQ=255)
                                                                                                                                     aCGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGtt   >  1:46726/1‑139 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAAt                                                              <  1:217363/79‑1 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAAt                                                              >  2:217363/1‑79 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCg                                                            >  5:400954/1‑81 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCg                                                            <  6:400954/81‑1 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACtt                                             >  2:348586/1‑96 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACtt                                             <  1:348586/96‑1 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGc                                         >  4:351174/1‑100 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGc                                         <  3:351174/100‑1 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGttt  >  4:160279/1‑139 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGttt  >  3:353552/1‑139 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGttt  >  1:110202/1‑139 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGtt   >  4:194937/1‑138 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGtt   >  1:294211/1‑138 (MQ=255)
                                                                                                                                      cGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGAGACCAAAAGGATGGTCATGATCGGGCGGttt  >  6:105053/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GCCTTTATGATCGTACGCAATCGCTATAATCGCTTTGCTGTTCCATTTTTTCCCTTGCTTTATAGAATAACGATATAAGATTTCCTTAGCCACGTACGAAATCAGAGCCGCAAACAATGCGCTGTATTGGGGAACGCTTGGCCCTTTAACAAAGGACAGAATCGCTTCTATGAGGATATAAACGGATACGATCACTAAGATAATTCCTACAATCGCCTCACTAATGACTTCAGCTTTTCCGTGACCAAAAGGATGGTCTTGATCGGGCGGTTT  >  CP006881/558571‑558843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑