| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 653,393 | T→G | 12.9% | intergenic (+17/‑5) | U712_03195 → / → U712_03200 | Uncharacterized protein yeaD/Uncharacterized protein yebA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 653,393 | 0 | T | G | 12.9% | 96.5 / 2.9 | 50 | intergenic (+17/‑5) | U712_03195/U712_03200 | Uncharacterized protein yeaD/Uncharacterized protein yebA |
| Reads supporting (aligned to +/- strand): ref base T (17/26); new base G (5/2); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTGGCAAAAGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCTTTTTTAGTG > CP006881/653255‑653530 | tACTGGCAAAAGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGt > 7:8075/1‑139 (MQ=255) gCAAAAGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTca‑ca > 3:79372/1‑139 (MQ=255) gCAAAAGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTca‑ca > 1:8731/1‑139 (MQ=255) aaGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGGGTTGTTTAGAAACGGAGGTGACTGGGTCA‑CATGcc > 2:370762/1‑139 (MQ=255) aaaGATGCTGAG‑‑CTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACgat < 2:221484/139‑1 (MQ=255) aGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACgat < 1:207912/139‑1 (MQ=255) gATGGTGATCTCTCTCAAGCTTAAAATGTCATTTTTGATCGCATGTTGTAAGTCCAAATCAGAGATGGGATCATGTGGGGCGGGCGTGTTTCGAGTGTTGTTTTGAAAGTGAGGTGTCTTGTTCA‑CATGCCGCACgat < 2:160098/138‑1 (MQ=255) tctcAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAATGAGAGGTGACTGGTTCA‑CATGCCGCACGAGGATCACAAggg > 4:300753/1‑137 (MQ=255) tctcAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGaa < 2:8731/139‑1 (MQ=255) tctcAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGGGTGGTTTTGAAAGAGAGGTGCCTGGTTCC‑CATGCCGCACGATGATCACAAggg > 6:265166/1‑137 (MQ=255) ctcAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTATCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAg < 4:175913/139‑1 (MQ=255) aaaaTGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAg > 2:26/1‑139 (MQ=255) aaaaTGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGGTGTTTAGAAAGAGAGGTGACTGGTTGA‑CATGCCGCCCGCTGCTCACAAGGGAAGCCGTTTGAg > 4:355203/1‑139 (MQ=255) tGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTg < 1:370762/139‑1 (MQ=255) tGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTg < 4:348284/139‑1 (MQ=255) aTGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTg < 1:26/138‑1 (MQ=255) gATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGtta > 3:389673/1‑139 (MQ=255) atgatgAAACGCCAAATCCGAGTTCGGATCCTGAGGGGCGGGCGAGTTTCGCGTGGTGTTTAGAAAGAGCGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTc < 4:870/139‑1 (MQ=255) gatgaAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGGTTTTAAAGAGAGGGGACTGGGTGACCAGGCGCCACGA‑GATGACAATGGAACCCGTTTGAGGTTGGGGTTTTTTTATTTTTTg > 4:221419/1‑139 (MQ=255) tgaAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGc < 4:79372/139‑1 (MQ=255) aaaCGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCtt < 4:105297/139‑1 (MQ=255) gCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGGGGTGGTTAGAAATCCAGGTGGATGGGTCA‑CAGGCCGCACGCTGCTAACAACGGGAGCCGGTTTAGGATGGTATGATTTTTTTTTGTGGGgttttt > 6:52771/1‑139 (MQ=255) ccAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTa < 5:265166/139‑1 (MQ=255) cAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTAt < 7:226728/139‑1 (MQ=255) cAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTAt < 4:389673/139‑1 (MQ=255) agTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTgg > 5:172675/1‑139 (MQ=255) agTTCGGATCATGAGGGGCGGGCGAGTTTCGCGGGTTGTTTTGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGACGCCGTTTGAGGTTGCGGTTTTTTTATTTTCTGGCTTTTTTATTGCTGTgg > 5:35058/1‑139 (MQ=255) ttCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa < 1:67086/139‑1 (MQ=255) ttCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa < 6:172675/139‑1 (MQ=255) ttCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa < 5:403555/139‑1 (MQ=255) ttCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCAGGCTTTTTTATTGCTGTGGGa < 5:319928/139‑1 (MQ=255) gtCGGAGCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa < 5:52771/138‑1 (MQ=255) aTGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa > 5:161893/1‑131 (MQ=255) aTGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGa < 6:161893/131‑1 (MQ=255) aTGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCt > 6:160969/1‑137 (MQ=255) aTGAGGGGCGGGCGAGTTTCGCGGGTTGTTTAGAAAGAGAGGGGGCTGGGTCA‑CCTGCCGCACGCTGATCACAAGGGAAGCCGTTTGAGCTTGCTGTGATTTTATTTTCTGGCTGTTTTATTGCTGTGGGGGTGGCt > 4:169004/1‑137 (MQ=255) aTGAGAGGAGGGCTATTTTCTCGTGTTGTTTATAAACAGAGGTGACTGGTTCA‑CATCCAGCACGATCATCACAAGGGAAGCCGTTATAGCATGCTGTTATATTATTTTCTGTGTTTTTTAATGCTTTGGGAGTGTCt > 6:175223/1‑137 (MQ=255) gcgggcgAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTTTTGCGGTGGGAGTGGCTTTGGgcgcg > 5:10176/1‑138 (MQ=255) gcgggcgAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGGTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCt > 7:321759/1‑139 (MQ=255) gggcgAGTTTCGTGTGTTGTTTAGTTCGAGATGTGACTGGTTCA‑CATGCCGCACGATGAGCTAAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGTGATTGGCTTAGGCCGCTTg < 8:329054/139‑1 (MQ=255) ggcgAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTa > 8:318435/1‑110 (MQ=255) ggcgAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTa < 7:318435/110‑1 (MQ=255) tttAGAAAGAGAGGTGACTGGGTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTgaaacgaa < 8:321759/139‑1 (MQ=255) tAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTgaaacgaaa > 7:57199/1‑138 (MQ=255) aaaGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATa < 8:8075/139‑1 (MQ=255) agagagGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATa > 2:113430/1‑137 (MQ=255) agagagGTGACTGGGTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACa < 3:300753/139‑1 (MQ=255) aCTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCttttt > 1:18861/1‑138 (MQ=255) gTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCTTTTTTAgtg < 3:355203/139‑1 (MQ=255) gTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCTTTTTTAgt < 3:169004/138‑1 (MQ=255) gTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCTTTTTTAgt < 5:175223/138‑1 (MQ=255) | TACTGGCAAAAGAGAAAGATGCTGAGCTCTCTCAAGCTGAAAATGTCATGATTGAACGCATGATGAAACGCCAAATCAGAGTTCGGATCATGAGGGGCGGGCGAGTTTCGCGTGTTGTTTAGAAAGAGAGGTGACTGGTTCA‑CATGCCGCACGATGATCACAAGGGAAGCCGTTTGAGCTTGCTGTTATTTTATTTTCTGGCTTTTTTATTGCTGTGGGAGTGGCTTAGGCCGCTTGACAGTTTTACTGAAACGAAACATACAGGCTTTTTTAGTG > CP006881/653255‑653530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |