Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,387 A→T 100% T67T (ACA→ACT)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,3870AT100.0% 185.4 / NA 55T67T (ACA→ACT) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/37);  total (18/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGT  >  CP006881/3723253‑3723514
                                                                                                                                      |                                                                                                                               
aGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTACCGGCAGCCGCTGTCACTTTCg                                                                                                                             >  7:35079/1‑139 (MQ=255)
 gCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            <  2:19281/139‑1 (MQ=255)
 gCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            <  8:35079/139‑1 (MQ=255)
    aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGc                                                                                                                         <  6:240104/139‑1 (MQ=255)
    aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGc                                                                                                                         <  7:106557/139‑1 (MQ=255)
      ctCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTGTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  6:226703/138‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCCGCCGCTGTCACTTTCGGGGCCg                                                                                                                       >  8:376011/1‑139 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  2:109388/139‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  2:2140/139‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  3:272072/139‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  3:271832/139‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  3:105881/139‑1 (MQ=255)
      aTCCGTTATGTGCTTCGGTCCATTTTGGACAGTATATTTATTGGTTTTGGGATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                                                       <  1:256792/139‑1 (MQ=255)
           ttATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                                                  >  3:58264/1‑139 (MQ=255)
           ttATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                                                  <  2:299122/139‑1 (MQ=255)
                  cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatg                                                                                                            <  4:349495/132‑1 (MQ=255)
                  cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCtgatga                                                                                                           <  7:63691/133‑1 (MQ=255)
                  cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCtgatga                                                                                                           <  5:319844/133‑1 (MQ=255)
                     cggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTgatgat                                                                                                          <  6:223052/134‑1 (MQ=255)
                     cggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTg                                                                                                        >  7:106462/4‑139 (MQ=255)
                                ggCCAGTTTATTTATTGGGTTTGGCATTACAGCAGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTAACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAgg                                                                                              <  1:350950/138‑1 (MQ=255)
                                 gCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg                                                                                            <  3:386911/139‑1 (MQ=255)
                                  ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg                                                                                            >  1:324346/1‑138 (MQ=255)
                                           ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatttatt                                                                                  >  4:320742/1‑139 (MQ=255)
                                                     tggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                                                         <  4:58264/137‑1 (MQ=255)
                                                      ggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAaca                                                                       >  6:152715/1‑139 (MQ=255)
                                                            aCAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            >  1:270952/1‑80 (MQ=255)
                                                            aCAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            <  2:270952/80‑1 (MQ=255)
                                                              aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                               <  7:245425/139‑1 (MQ=255)
                                                              aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATGCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCTGAGATTTATTTACCGGCAACACCTTTTAt                                                               <  8:283353/139‑1 (MQ=255)
                                                                ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAttt                                                             <  5:202308/139‑1 (MQ=255)
                                                                ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAttt                                                             <  2:324346/139‑1 (MQ=255)
                                                                ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAtt                                                              >  5:6441/1‑138 (MQ=255)
                                                                ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAtt                                                              >  3:80422/1‑138 (MQ=255)
                                                                     agcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGCTGATTGCTTTCGGCGGCGGGGCTTTATTTACCGGGAACACCGTTTATTTTAcc                                                         >  6:9746/1‑137 (MQ=255)
                                                                              ggCAGCTATTTCATTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTATCGCGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCgcgc                                               <  7:186267/139‑1 (MQ=255)
                                                                               gCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCt                                              >  3:57478/1‑139 (MQ=255)
                                                                                             aTGGCTGATTCTCCGTTTGCCTTTCCGGAAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTAGTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAt                                  >  8:53386/1‑137 (MQ=255)
                                                                                                    aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            <  3:111907/40‑1 (MQ=255)
                                                                                                    aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                                            >  4:111907/1‑40 (MQ=255)
                                                                                                    aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTTCCGGCaa                                                                         >  3:259827/1‑91 (MQ=255)
                                                                                                    aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                                                         <  4:259827/91‑1 (MQ=255)
                                                                                                       ctacGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCTCCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                       <  6:367723/135‑3 (MQ=255)
                                                                                                          cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                      <  6:116441/136‑1 (MQ=255)
                                                                                                          cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                      <  5:162214/136‑1 (MQ=255)
                                                                                                          cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                    <  8:106462/138‑1 (MQ=255)
                                                                                                          cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCCGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                      <  3:320742/136‑1 (MQ=255)
                                                                                                              tGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta               >  1:325675/1‑138 (MQ=255)
                                                                                                                    tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                               >  7:236147/1‑85 (MQ=255)
                                                                                                                    tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                               <  8:236147/85‑1 (MQ=255)
                                                                                                                    tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                               <  5:311212/85‑1 (MQ=255)
                                                                                                                    tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAt                                                               >  6:311212/1‑85 (MQ=255)
                                                                                                                      cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgtg       <  4:80422/139‑1 (MQ=255)
                                                                                                                      cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgtg       <  6:6441/139‑1 (MQ=255)
                                                                                                                           gCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGt  <  5:9746/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                               
AGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGT  >  CP006881/3723253‑3723514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑