Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,573	A→G	100%	T129T (ACA→ACG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,573	0	A	G	100.0%	181.1 / NA	49	T129T (ACA→ACG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/19);  total (30/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTC  >  CP006881/3723437‑3723706                                                                                                                                         |                                                                                                                                      ccggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCTGCGGGCGCGACACCATATACTTGTGGATGTCAAGCTATGCCGGCAATTTATTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                                     <  1:369572/137‑1 (MQ=255) ccggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                                     <  7:53386/137‑1 (MQ=255)        cacCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                                                                                                                              <  7:11571/139‑1 (MQ=255)           cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                                           <  4:120000/138‑1 (MQ=255)                   tCACCTATACCGCTCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                    <  1:208449/138‑1 (MQ=255)                   tCACCTATACCGCGCTCCGTAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                    <  7:346814/138‑1 (MQ=255)                               cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                    <  6:22629/126‑1 (MQ=255)                               cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                    >  5:22629/1‑126 (MQ=255)                               cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                                       >  7:68845/1‑139 (MQ=255)                                 cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                                         >  5:377827/1‑119 (MQ=255)                                 cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                                         <  6:377827/119‑1 (MQ=255)                                     ggaaaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                                                                 <  2:325675/137‑1 (MQ=255)                                              tcagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTgg                                                                                         >  2:124025/4‑138 (MQ=255)                                                                tatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgcc                                                                      >  5:112537/3‑139 (MQ=255)                                                                     ttgtgGATGTCAGGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                                                       <  7:292607/116‑1 (MQ=255)                                                                     ttgtgGATGTCAGGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                                                       >  8:292607/2‑117 (MQ=255)                                                                       gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGATCAGCCGGCt                                                               >  1:312103/1‑139 (MQ=255)                                                                       gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt                                                               >  7:305348/1‑139 (MQ=255)                                                                       gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt                                                               >  8:62510/1‑139 (MQ=255)                                                                        tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTGATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGccgccggcgt                                                              >  1:134972/1‑137 (MQ=255)                                                                        tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                              >  7:267899/1‑139 (MQ=255)                                                                        tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGAGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTTATTAATTTGGGAGAGCCGAAAATGGCGCCGCCGGCtt                                                              >  7:170732/1‑139 (MQ=255)                                                                          gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCATAGCACAAAATGGAGCCGCCGGCTTcc                                                            >  2:323223/1‑139 (MQ=255)                                                                          gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                            >  4:162023/1‑139 (MQ=255)                                                                            tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGTAGCCTTCTGTTCATTCCTTTTTGATTCATTTTGCAGAGCACAAAATGGAGCCGCCGGCTTCCGa                                                          >  1:256948/1‑139 (MQ=255)                                                                              tCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaa                                                         <  3:162023/138‑1 (MQ=255)                                                                                      tGCCGGTAATTTAATCGGCGCCATTCTGTTTCCCTTCCTGAGCCGCGGGACGGGACTTTTGGTGGTGCCTTCTTTTCATTCCTTTTTGATTCATTTGGCTGAGCAAAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 <  2:312103/138‑1 (MQ=255)                                                                                      tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                      >  7:393715/1‑133 (MQ=255)                                                                                      tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                      <  8:393715/133‑1 (MQ=255)                                                                                      tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 <  7:365500/138‑1 (MQ=255)                                                                                      tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 <  4:57478/138‑1 (MQ=255)                                                                                       gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 >  3:68937/1‑137 (MQ=255)                                                                                       gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCCCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAAAATGGAGCCGCCGGCTACCGAATTGTTTTTCa                                               >  4:188888/1‑139 (MQ=255)                                                                                                         gcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                             >  8:283662/1‑139 (MQ=255)                                                                                                                  tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGCGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt                    >  1:277068/1‑139 (MQ=255)                                                                                                                     gCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc                 >  6:158524/1‑139 (MQ=255)                                                                                                                       cATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 >  2:390127/1‑105 (MQ=255)                                                                                                                       cATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCAAAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                 <  1:390127/105‑1 (MQ=255)                                                                                                                         tccTGATAAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTAATTTGGAAGAGCACAAAATCGAGCCGCAGGCGACCGAAATGTTTTTCATAGGAATGCTTTGCAATTCGCATGTGTGCCTctc             >  1:350691/3‑137 (MQ=255)                                                                                                                            tGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtt          >  6:387264/1‑139 (MQ=255)                                                                                                                            tGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCGTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtt          >  8:96833/1‑139 (MQ=255)                                                                                                                              aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt        >  7:360893/1‑139 (MQ=255)                                                                                                                              aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt        <  7:62510/139‑1 (MQ=255)                                                                                                                               tCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt       <  7:283662/139‑1 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                                                                 >  7:265696/1‑45 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                                                                 <  8:265696/45‑1 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGTATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt   >  2:75505/1‑139 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt        >  1:357922/1‑134 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt        <  2:357922/134‑1 (MQ=255)                                                                                                                                   cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAt    >  6:269046/1‑138 (MQ=255)                                                                                                                                    gcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTc  <  3:53982/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTC  >  CP006881/3723437‑3723706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑