BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	121	G→C	5.5%	intergenic (–/‑2857)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	121	0	G	C	5.5%	34850.8 / 269.4	12295	intergenic (–/‑2857)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (5335/5687); new base C (4/872); total (5350/6945)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 12501 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGaa                                                                                                                                          <  8:42676‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGaa                                                                                                                                          <  1:251953‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:131344‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGaa                                                                                                                                          <  2:291342‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:256006‑M1/123‑1 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  6:150148‑M1/127‑1 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  6:382735‑M1/127‑1 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGACCGGa                                                                                                                                      <  7:137484‑M1/127‑1 (MQ=255)

        ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTATTAGAAACATAGCGGTAAAA‑TTATCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  5:285662‑M1/130‑1 (MQ=255)

         tcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATg                                                                                                                                  <  8:192090‑M1/131‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  2:38689‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  8:243224‑M1/132‑1 (MQ=255)

           aataatCCAACCTCCATAGTTGGAGAGTGAGTTTTATGTCGCAATTATTATTGTTTCTGGTGAACCTTATCAATTTTTCGTTTATTTAATAGAAACATAGCGGAAAAA‑TTACCAGAAACTTAATAGAACGGAAATTaaa                                                                                                                               <  8:272192/134‑1 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  3:358837/1‑139 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGAAGTAAGTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  1:100058/1‑139 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAAATTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  1:363559/1‑139 (MQ=255)

                accATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTTATTAAATAGAAACATAGCGGTAAAA‑TTAGCAGAAACTTAATAGAACGGAAATTAAAAAAGc                                                                                                                          <  5:144687/138‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  3:165942/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  3:188414/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  5:84711/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  2:59146/1‑139 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  2:262497/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGTAAATTAAAAAAGCCAct                                                                                                                      <  6:116492/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  8:319198/1‑139 (MQ=255)

                    tccaccAATGTTGGATAGTGTTTTTTATGTCGAAAATATTATTGTTTTTGGGTCACCTTATAAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAA‑TTAGCAGTAACTTAAAACAACGCAAATTAAAAAAGCCAct                                                                                                                      <  4:159875/139‑1 (MQ=255)

                         cAAAGTTGGTTCGTGAGTTTTATGTCGAAAATATTATTGTTTCTGGTTAACCTTATCAATTTTTCGTTTATTTAATCCAAACATAGCGGAAAAA‑TTAGCACTAACTTAATAGAACGTAAATTAAAAAAGCCACTCTCat                                                                                                                 <  4:103185/139‑1 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACtctc                                                                                                                   <  6:82999/137‑1 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACtctc                                                                                                                   <  5:340691/137‑1 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACtctc                                                                                                                   <  7:165807/137‑1 (MQ=255)

                          aaaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                <  5:193219/139‑1 (MQ=255)

                           aaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCatat                                                                                                               >  7:309526/1‑139 (MQ=255)

                            aGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAA‑TTAGCAGTAACTTAATAGACCGGAAATGAAAAAAGCCACTCTCATATg                                                                                                              <  5:223289/139‑1 (MQ=255)

                                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  6:286643/1‑125 (MQ=255)

                                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                          >  7:50944/1‑139 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:239430/124‑1 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  1:62539/1‑139 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         <  1:303124/139‑1 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGATACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  6:211952/1‑139 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  5:254471/1‑139 (MQ=255)

                                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   >  7:385464/1‑138 (MQ=255)

                                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   >  7:7886/1‑138 (MQ=255)

                                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   >  2:15794/1‑138 (MQ=255)

                                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                   >  4:288820/1‑138 (MQ=255)

                                         tttttAGGTCGCAAATATTTATTTTTCTGGTCAACCTTATCAAATTTTGGTTTTTTTAATACAAACATTGCGGAAAAA‑TAAGCAGAAACTTAATAGACCGGAATTGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  2:31948/137‑1 (MQ=255)

                                         gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TAAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  5:116130/138‑1 (MQ=255)

                                         gtTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATCTAAACATAGCGGTAAAA‑TTAGCATTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  3:245930/138‑1 (MQ=255)

                                         gtTTTATGTCCCAATTATTAATTTTTCTGGTCAACCTTATCAAATTTTCTTTTATTAAAAATAAACATACCGCTAAAA‑TAAGCAGTAACTTAAAAGAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  2:23610/138‑1 (MQ=255)

                                             tATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                             >  7:215942/1‑139 (MQ=255)

                                                gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:110779/109‑1 (MQ=255)

                                                gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                            >  8:104904/1‑137 (MQ=255)

                                                  cccAAATATTAATTTTTCTGGTGAACATTATCAAATTTTCTATGATTAAATAGAAAAATAGCGGTAAAA‑TTAGCAGAAACTAAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        <  8:217058/137‑1 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGctg                                                                                      >  3:217730/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  4:227203/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  5:167565/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGATACCAACCTTTAGc                                                                                        >  3:140379/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCCCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  5:38968/1‑137 (MQ=255)

                                                       atatAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGAAAAA‑TTAGCAGAAAATTAATAGAACGGAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  1:393128/139‑1 (MQ=255)

                                                        tatTAATTTTTCTGGTGAACCTTATCAAATTTTCTTTGATTTAATAGAAACATAGCTGAAAAA‑TTAGCATTAACTTAATAGAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  8:349211/138‑1 (MQ=255)

                                                             aTGTTTCGGGTGACCCTGATCAAAATTGCGTACATAAAATCGAAACTTCGCCGAAAAC‑TACCCTGTAACTACAAAGCCCGGAAATGAAAACAGCCACTCTCATATGCTCTTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  8:70231/138‑1 (MQ=255)

                                                              tGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTa                                                                            <  2:8954/139‑1 (MQ=255)

                                                                tttCTGGTTACCCTAATAAAATTTTCTTTCATTAAATACAAACATCGCGGTAAAA‑TTACCTTAAACTAATTACACCGAAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  6:348932/138‑1 (MQ=255)

                                                                tttCTGGTTAACCTTATCAAATTTTTGGTTATTTAAAATAAACAAAG‑GGTAAAATTTGGCATTAAATTATTAGAACGCAAATTAAAACCGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  3:78004/138‑1 (MQ=255)

                                                                tttCTGGTGAACCTTATCAAATTTTGTTTGATTTAATAGAAAAAAAGCGGTAAAA‑TTAGCAGTAACTAAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  4:190526/138‑1 (MQ=255)

                                                                 ttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         >  1:22446/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  7:358685/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   >  6:181687/1‑128 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  3:52419/128‑1 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  3:115798/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  7:332371/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  2:120043/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:366048/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:31171/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         <  8:293311/138‑1 (MQ=255)

                                                                  tCTGGTGAACCTTATAAAATTTTCGGTGATATAAAAAAAACATAGCGGAAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         <  6:44387/138‑1 (MQ=255)

                                                                  tCTGGTCAACATTATCAAATTTTCGTTTATTAAATATAAACATAGCGTTAAAA‑TTAGCAGTAACTTAATACAACGTAAATAAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         <  1:193312/138‑1 (MQ=255)

                                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                       >  4:342598/1‑139 (MQ=255)

                                                                    tGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  8:131493/139‑1 (MQ=255)

                                                                      ttGAACTTTATAATATTTTCTTTTATTAAATATAAAAATAGGGGTAAAA‑TTAGCATTAACTTAATTGAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  8:162644/138‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTATCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTACCAGTAACTAAATAGACCGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  4:51095/139‑1 (MQ=255)

                                                                        gAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    >  3:274762/1‑137 (MQ=255)

                                                                         aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  3:327117/139‑1 (MQ=255)

                                                                              tATCTAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  6:315049/1‑79 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  3:302384/131‑1 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                            >  5:5460/1‑139 (MQ=255)

                                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTAAGCGAGAATGACTTAATCCTGTACAGCCATa                                                            >  1:132381/1‑137 (MQ=255)

                                                                                      tttCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    >  1:161145/1‑139 (MQ=255)

                                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                   >  4:8562/1‑139 (MQ=255)

                                                                                          gTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGAc                                                >  7:267852/1‑139 (MQ=255)

                                                                                                 tAATAGAAACATAGCGCTAAAA‑TTAGCAGTAACTTAATAGAACGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATaa                                          <  7:286592/138‑1 (MQ=255)

                                                                                                        aaCATAGCTTTAAAT‑TAAGCATTAAATTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  <  4:3178/139‑1 (MQ=255)

                                                                                                        aaCATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                   >  4:375778/1‑138 (MQ=255)

                                                                                                        aaCATAGCGGTAAAA‑ATAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTATAGCTAGAATCACTTAATCCTGTACAGCCATACAGGACTACTACTAATAAGAGgcgc                                   >  1:276552/1‑138 (MQ=255)

                                                                                                         aCATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  3:386030/102‑1 (MQ=255)

                                                                                                         aCATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    >  1:330166/1‑104 (MQ=255)

                                                                                                         aCATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                  >  1:89595/1‑138 (MQ=255)

                                                                                                              gCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                             >  6:248108/1‑138 (MQ=255)

                                                                                                                 gTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                         >  2:35371/1‑139 (MQ=255)

                                                                                                                           gaaGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                 <  8:377046/136‑1 (MQ=255)

                                                                                                                               tAACTTAATAGAACGGAAATCAAAAAAGACACTCTAAGAAGCTATAGGCTACCTCGATTTAGCTAGATTGACTTAATCCTCTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTAAAAATAAGTTATTTGcg            >  2:200965/1‑138 (MQ=255)

                                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  7:191022/81‑1 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAA‑TTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`