BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	131	G→C	6.8%	intergenic (–/‑2847)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	131	0	G	C	6.8%	35050.3 / inf	13037	intergenic (–/‑2847)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (6274/4765); new base C (23/958); total (6307/6730)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 13074 total aligned reads displayed.

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCT  >  pSIJ663_pHT315_araE_xy/1‑269

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATTTAGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGTTAAAATAAGCAGAAACTTAATAGAACGTAAATGa                                                                                                                                           <  5:304108‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                           <  8:340197‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                           <  2:352475‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATATAATAGAAACATACCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                           <  3:164766‑M1/132‑1 (MQ=255)

      tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAAGTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                    >  1:100058/1‑139 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCTCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  7:279383/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  7:376208/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  7:125594/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  7:230075/139‑1 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTTATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                >  4:277294/1‑139 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                >  3:244749/1‑139 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                >  1:395573/1‑139 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAAct                                                                                                                                >  2:202741/1‑139 (MQ=255)

               cAATGTTGGAGTTTTTGTTTTATGTCGCAAATTTTAATTTTTCTTGTGAAACTTATAAATTTTTCTTTTATTTAATATAAAAATAGCGGTAAAATTCGCATAAAATTAATAGAACGGAAATTAAAAAAGCCACTCTCat                                                                                                                           <  2:120061/139‑1 (MQ=255)

               cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAAATAGCAGTAACATAATAGAACGGAAATGAAAAAAGCCAAACTCa                                                                                                                            >  3:325910/1‑138 (MQ=255)

                aactttGGTGTGTTAGTTTTATGTCGAAAATTTTAATGTTTCTGGTCAACCTTATCAAATTTTCGTTTATTTAATAGAAACATCGCGTAAAAATTAGCAGTAAATTAATAGACCGGAAATCAAAAACGCCACTCTCata                                                                                                                          <  4:244290/135‑1 (MQ=255)

                aactctGGACAGGGAGTTTTCTGTCCAAAAAATTAATGTTTCTGGTGAACATTATCAAATTTGCGTTGATTAAATAGAAACATCCCGTTAAATTTCGCAGTAAATTAATAGAACGTAAATGAAAAACGCCACTCTCata                                                                                                                          <  6:102641/134‑1 (MQ=255)

                aaaTTTGTATAGTGAGTTTTATGTCGCAAATATTAATGTTTGTGGTTAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGAAAAATTAGCAGTAACTTAATATAAGGGAAATGAAAAAAGCCACTCTCata                                                                                                                          <  3:355272/139‑1 (MQ=255)

                    ttGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGc                                                                                                                       >  6:133639/1‑138 (MQ=255)

                       gtttgtgagtTTTATGTCGCAAATATTATTGTTTCTGGTCAACATTATAAATTTTTGGTTTATTTAATAGAAACATCGCGGTAAAATTAGCATTAACTTAATCGACCGGAAATGAAAAACGCCACTCTCATATGCTAtt                                                                                                                   <  6:273055/135‑1 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  >  8:210990/1‑124 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                   >  7:102163/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                   >  1:62539/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                   >  3:113981/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                              >  8:31241/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                              >  8:285352/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTa                                                                                                              >  1:197812/1‑139 (MQ=255)

                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                             >  5:132271/1‑138 (MQ=255)

                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTACCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTCGCTAc                                                                                                             >  8:184632/1‑138 (MQ=255)

                               gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                            <  5:70565/138‑1 (MQ=255)

                               gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTAATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAATAAGCAGTAAATAAATACACCGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                            <  5:10382/138‑1 (MQ=255)

                               gtTTTATGTCCCAATTATTAATTTTTCTGGTCAACCTTATCAAATTTTCTTTTATTAAAAATAAACATACCGCTAAAATAAGCAGTAACTTAAAAGAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                            <  2:23610/138‑1 (MQ=255)

                                 tttATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                                        <  7:255941/124‑1 (MQ=255)

                                    aTGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  3:301163/111‑1 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                  >  3:73816/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTAGATTTAATAGAAACTTAGCGGTATATTTAGCAGTACCTTAATAGAGCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCATCCTTTAGc                                                                                                  >  3:3839/1‑137 (MQ=255)

                                             gttttATTGTTTCTGGTGAACATTATCAAATTTTCTTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  2:56574/136‑1 (MQ=255)

                                             atatTAATTTTTCTGGTTAAGATTATCAATTTTTCTTTTATTTAATATAAAAATAGCGTTAAAATTCGCAGTAACTTATTTTAAGGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  4:124206/139‑1 (MQ=255)

                                             atatTAATTTTTCTGGTTAACCTTATAAAATTTTCGTTTATTTAATACAAACATAGCGTAAAAATTACCAGAAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  3:47983/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  1:332561/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  4:36483/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTCAACCTTAACAAATTTTCTTTGATTTAATAGAAACATAGCGGTAAAATTCGAAGTAACTAAATAGAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  4:151320/139‑1 (MQ=255)

                                             atatTAAGTTTTATGGTCATCATTAGATAATTTTGTTTTATTAATTAGTAACATAGCTTTAATATTCCCATAAATTAAATAGCAGGTAATCCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             <  4:39899/139‑1 (MQ=255)

                                                   attgttCTGGTGAACCTTTTCAAAATTTCGTGTATTAAATAGAAACATACCGGTAAAATAAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACtt                                                                                       <  5:328069/135‑1 (MQ=255)

                                                   aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                        <  5:380462/138‑1 (MQ=255)

                                                   aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                        <  7:302068/138‑1 (MQ=255)

                                                   aTGTTTCGGGTGACCCTGATCAAAATTGCGTACATAAAATCGAAACTTCGCCGAAAACTACCCTGTAACTACAAAGCCCGGAAATGAAAACAGCCACTCTCATATGCTCTTGGCTACCAACCTTTAGCGAGAATGACt                                                                                        <  8:70231/138‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTa                                                                                      >  5:210461/1‑135 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                  >  1:132757/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                  >  1:279154/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                  >  3:139795/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                  <  5:355692/91‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAAGAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACAAACCTTTAGCGAGAATGACTTAATcc                                                                                  >  5:276123/1‑139 (MQ=255)

                                                        tCTGGTGAAACTTATAAAATTTTCGTTTATTTAATATAAAAATATCGTTAAAATTAGCAGTAACTTAATAGAACGGATATGAAAAAATCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                   <  7:206656/138‑1 (MQ=255)

                                                           ggTGAACCTTATCAAATTTTATTTGATTTATTATAAACTTAGCGGTTAAATTATAAGAAACTTAATAGAACTGAAATGAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                <  6:164129/138‑1 (MQ=255)

                                                            gttaaCCTTATAAAATTTTCGTTGATTAAATATAAAAATAGCGGTAAAATTAGCAGTAAATTAATACAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              <  4:307649/136‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTTATTAAATAGAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              <  6:33259/139‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              <  7:155347/139‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              <  3:274719/139‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACAAAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                <  1:25383/137‑1 (MQ=255)

                                                            gTGAACATTATAAAATTTTCGTTGTTTTAATATAAAAATCGCGTTAAAATTAGCTTTAACTTAATAGATCGGAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              <  2:319186/139‑1 (MQ=255)

                                                               aaCCTTCTCACATTTTCGTGTCTTTCAAAGAAACACCGCGCTAACATACGCAGTACTTCAAGAGACCGGAACTCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGACTGACTTAATCCTGTACAg                                                                           <  4:72364/139‑1 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                           >  2:159927/1‑139 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACAAAGCGGTAAAATTAGCAGTAACTAAATAGAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                           <  7:181237/139‑1 (MQ=255)

                                                               aaCATTATCAAATTTTGTTTTTTTTAATAGAAAAATAGCTTTAAAATTACCATTAATTATATATAACTCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                           <  6:36973/139‑1 (MQ=255)

                                                                     aTCAAATTTTAGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                             >  2:38513/1‑115 (MQ=255)

                                                                      tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                     <  8:122608/122‑1 (MQ=255)

                                                                      tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                     <  6:303599/138‑1 (MQ=255)

                                                                      tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACATTTACCGAGAATCACTTAATCCAGTACAGCCATa                                                                      >  3:187833/1‑137 (MQ=255)

                                                                           ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                               >  8:153986/1‑139 (MQ=255)

                                                                             ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                              <  8:233867/138‑1 (MQ=255)

                                                                             ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                              <  2:293143/138‑1 (MQ=255)

                                                                             ttCGTTGATTTAATAGAAACATAGCGGAAAAATTAGCAGAAACTTAAAAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                              <  6:196802/138‑1 (MQ=255)

                                                                                              aaaaaaGCGGTAAAATTAGCAGTAACTTAATAGACCGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                            <  8:260409/134‑1 (MQ=255)

                                                                                              aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                             >  8:400103/1‑138 (MQ=255)

                                                                                              aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                            <  7:41673/139‑1 (MQ=255)

                                                                                               aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATAGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                            >  2:88273/1‑138 (MQ=255)

                                                                                                   aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                <  7:95792/98‑1 (MQ=255)

                                                                                                       gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCTCCAACTTCaa                                    >  1:164258/1‑138 (MQ=255)

                                                                                                       gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                   >  4:182885/1‑139 (MQ=255)

                                                                                                       gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                   >  1:90129/1‑139 (MQ=255)

                                                                                                                    tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                      >  1:36655/1‑139 (MQ=255)

                                                                                                                      aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                        <  6:397895/71‑1 (MQ=255)

                                                                                                                            tacaccGGAAATCAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc              <  6:232508/134‑1 (MQ=255)

                                                                                                                            tAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACGGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc              >  8:372024/1‑139 (MQ=255)

                                                                                                                                aCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCACCTTCAAATAAGTTATTTGCCTTGTTTTcgcg           >  4:213216/1‑138 (MQ=255)

                                                                                                                                aCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgcg           >  7:68563/1‑138 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                              >  3:216147/1‑70 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa         >  1:306096/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa         >  5:156823/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa         >  5:241715/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa         >  1:227402/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATCCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGCACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGACTTGTATTCGCGaa         >  6:68053/1‑139 (MQ=255)

                                                                                                                                 aggAAATGAAAAAAGCCACTCTCATAAGCTAATGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTACGACTTATAAGACCCGCCAACTTCAAATAAGTTATTTGCCTAGTTTTCGCGaa         >  5:183/2‑139 (MQ=255)

                                                                                                                                  gcaaaTCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTCGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc        <  2:263732/137‑1 (MQ=255)

                                                                                                                                   gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa       <  4:335463/139‑1 (MQ=255)

                                                                                                                                   caaaTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa       <  3:95565/138‑1 (MQ=255)

                                                                                                                                    aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc        >  7:32621/1‑137 (MQ=255)

                                                                                                                                    aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc        >  5:404418/1‑137 (MQ=255)

                                                                                                                                     aaTAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa       <  7:352849/137‑1 (MQ=255)

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCT  >  pSIJ663_pHT315_araE_xy/1‑269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`