BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	137	A→C	5.4%	intergenic (–/‑2841)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	137	0	A	C	5.4%	31865.3 / inf	12612	intergenic (–/‑2841)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (6225/5417); new base C (49/893); total (6291/6321)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 13124 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

tcaaTCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATTGTTCTGTTTAACCTTATAAAATTTTCGTTTATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGc                                                                                                                                          <  8:313369/136‑1 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  3:190680/1‑139 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAAGTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  1:100058/1‑139 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTCAACCTTAACAAATTTTCGTTGATTAAATACAAACATAGCGTTAAAATTCCCAGTAACTTAATATAACGGAAATGAAAAAAGc                                                                                                                                          <  8:123583/139‑1 (MQ=255)

  caacctccAAAGTTGTAGAGTGAGTTTTATGTCGCAATTATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTAAAAATTAGCAGTAAGTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:304627/136‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  3:14065/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  2:201226/1‑139 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  6:305619/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  3:330562/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACTGAAATGAAAAAAGCCAct                                                                                                                                      >  5:250017/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACTGAAATGAAAAAAGCCAct                                                                                                                                      >  1:334396/1‑139 (MQ=255)

    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      >  7:69683/1‑139 (MQ=255)

    tcctccAAAGTGGGAGAGTTTGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGTTAAAATTAGCAGTAAATTAATAGAACGTAAATTAAAAAAGCCAct                                                                                                                                      <  4:102099/139‑1 (MQ=255)

       tccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGATACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAATAAAGCCActct                                                                                                                                    >  1:398492/1‑138 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                                  >  8:286573/1‑138 (MQ=255)

         aatatttGTAGAGTTATTTTTATGTCCAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTAAATATAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGCAAATTAAAAAAGCCACTCTCat                                                                                                                                 <  2:263826/134‑1 (MQ=255)

          aaaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCata                                                                                                                                <  6:139327/139‑1 (MQ=255)

          aaaGTTGCTGAGTGTGTTTAATCTCCCAAATATTAATTTTTCTGGTGAACTTTATCAAATTTTCGTTGATTTAATATAAACATAGCGTTAAAATTACCATAACCTTAATACACGGTAAATCAAAAAAGCCACTCTCata                                                                                                                                <  4:373470/139‑1 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      <  3:166140/127‑1 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                                          >  6:200687/1‑139 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  5:138736/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  8:373144/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  7:155164/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  6:108096/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATCCTATTGGATAc                                                                                                                   >  3:280453/1‑138 (MQ=255)

                        agtTTTATGTCGAAAATATTAATGTTTCTGGTGAACATTATAAAATTTTCGTTTATTTAATATAAACATAGCGGAAAAATTACCAGTAACTTAATAGAACGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  1:93032/139‑1 (MQ=255)

                         gtTTTATGTCTCAAATATTAATGTTTCTGGATAACATTATAAAATTTTCTTTTATTTAATACAAACATAGCGTTAAAATTAGAAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  1:63930/138‑1 (MQ=255)

                              aTGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                            >  3:219140/1‑139 (MQ=255)

                                  ccaaaaTTTTAATTTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATACAAACATCGCGTAAAAATTACCAGTAAATTAATATAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  3:30496/136‑1 (MQ=255)

                                   gaaaaTATTAATGTTTCTGGTTAACATTATAAAATTTTCGTTGATTAAATACAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  2:318967/136‑1 (MQ=255)

                                   gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  1:254420/1‑138 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  3:53508/1‑137 (MQ=255)

                                       gttttATTGTTTCTGGTGAACATTATCAAATTTTCTTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  2:56574/136‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  7:42297/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  3:78568/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTAGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  5:188004/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTAATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  5:45269/139‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  5:360437/138‑1 (MQ=255)

                                                tttCTGGTTAACATTATAAAATTTTCGTTTATTTAATACAAACATAGCTCAAAAATAAGAATAAAATAAATACACCGAAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  4:4586/138‑1 (MQ=255)

                                                attCTGGTTAACCTTAACAATTTTTCGTTTATTTAATAGAAACATAGCGGAAAATTTACCAGTAACTTAATAGAACGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  5:307799/137‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           >  5:77615/1‑136 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  7:25703/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:281577/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:196022/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  5:16382/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  7:9653/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATca                                                                                        >  5:219688/1‑138 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         >  1:162839/1‑138 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  3:360416/112‑1 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  6:275917/127‑1 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  7:181142/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  5:110968/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  7:352873/1‑139 (MQ=255)

                                                      gttaaCCTTATCAAATTTTCGTTTATTTAATAGAAACAAAGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  4:130891/136‑1 (MQ=255)

                                                      ggtaaacTTTTCAAATTTTGTTTTATTTAATATAACATTTCCGTTAAAAATCCCAATAACTTAATACAACGTAAATAAAACAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  2:385930/133‑1 (MQ=255)

                                                      gctaaCATTATAAAATTTTAGTTGATTTATAATAAACATAGCGTTAATTTTCGGAGTAACTTAATCCACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  4:25204/136‑1 (MQ=255)

                                                      gTGAACTTTATAAATTTTTCGTTGTTTTAATACAAACATAGTGGTAAATTAAGCATTAACTAAATTTACCCCAACCTAACACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  6:235895/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  5:384757/139‑1 (MQ=255)

                                                         aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  8:211339/1‑84 (MQ=255)

                                                         aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  6:369823/116‑1 (MQ=255)

                                                          aCCTTATAAAATTTTCGTTTTTTTAATAGAAACAAACCGGAAAAATTACCAGTAACTTAATACACCGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  4:7970/138‑1 (MQ=255)

                                                          aCCTTAGCAACTTTTCGTTGTTTTAATAGAAACATAGCGGTAAACTTCGCAGTAACTGAATCGCACGCAAATGAAAAACGCCACTCTCATATGCTCTTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                                <  7:279792/139‑1 (MQ=255)

                                                               aTCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  7:100915/1‑138 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  7:207251/114‑1 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                                   <  3:251181/130‑1 (MQ=255)

                                                                       ttCGTTTATTTAATAGAAACATCCCGTTAAAATTAGCAGTAACTTAATAGACCGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  4:341699/138‑1 (MQ=255)

                                                                       ttCGTTTATTTAATACAAAAATAGCGTTAAAATTATCATTAAATTAATAGAAGGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  2:352404/138‑1 (MQ=255)

                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  2:293143/138‑1 (MQ=255)

                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  8:154550/138‑1 (MQ=255)

                                                                          gTTTATTTAATACAAACATAGGGTTAAAATAAGCTTTAACTTATTCCAACGGAAATAAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                                                 <  2:162496/138‑1 (MQ=255)

                                                                                        aaCATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  <  7:124464/139‑1 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATAGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  2:88273/1‑138 (MQ=255)

                                                                                               cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                                            >  5:336015/1‑138 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  4:314232/1‑139 (MQ=255)

                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    >  1:227659/1‑109 (MQ=255)

                                                                                                       ttAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGt                                    >  6:169503/1‑138 (MQ=255)

                                                                                                              tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc                            <  8:26241/139‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  6:397895/71‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              >  7:231121/1‑71 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            <  4:285853/89‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  3:114324/1‑139 (MQ=255)

                                                                                                                aCTGAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCAGACAGGACTTCGCCTTATAATAGGCGCCCACTTCAAATAAGTTATTTGCCtg                          >  6:33387/1‑138 (MQ=255)

                                                                                                                        gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  3:38898/68‑1 (MQ=255)

                                                                                                                         aaCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                  >  2:236246/1‑138 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  1:30709/86‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:235276/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  5:282898/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:42176/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  1:367654/1‑139 (MQ=255)

                                                                                                                             gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  6:67141/139‑1 (MQ=255)

                                                                                                                             aaaaTCAAAAACTCCACTCTCATATGCTATTGGCTACCAACATTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  8:343515/138‑1 (MQ=255)

                                                                                                                              aaaacaaaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACaa            <  1:133839/134‑1 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  8:185553/67‑1 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACaa            >  4:157811/1‑139 (MQ=255)

                                                                                                                               aaTTAAAAACTCCACTCTCATATGCTATTGTCTACCAACCTTTAGCGAGAATTACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGATGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg           <  4:222548/139‑1 (MQ=255)

                                                                                                                                   aaaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtt       <  7:376310/139‑1 (MQ=255)

                                                                                                                                    aaaaaTCCACTCTCATATTCTATTGGCTACCAAACTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCATTGTTTTCGCGAACAAGGCtt       <  4:187488/138‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  7:32703/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  2:123094/139‑1 (MQ=255)

                                                                                                                                    aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  8:71907/139‑1 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`