BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,214	C→T	38.7%	intergenic (–/‑764)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,214	0	C	T	38.7%	17764.6 / inf	19035	intergenic (–/‑764)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (4723/6828); new base T (3001/4323); total (7813/11222)
Fisher's exact test for biased strand distribution p-value = 9.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 0.00e+00

Only 100 of 19138 total aligned reads displayed.

TAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGAT  >  pSIJ663_pHT315_araE_xy/2075‑2352

  aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                          <  5:231415/139‑1 (MQ=255)

  aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                          <  6:183510/139‑1 (MQ=255)

   aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGt                                                                                                                                          <  8:137480/139‑1 (MQ=255)

   aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                          <  1:296739/139‑1 (MQ=255)

   aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                          <  3:50920/139‑1 (MQ=255)

        ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCtt                                                                                                                                     >  8:224377/1‑139 (MQ=255)

        ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                                                                     >  7:171376/1‑139 (MQ=255)

        ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTAGTCTAtt                                                                                                                                     >  6:393109/1‑139 (MQ=255)

                     cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                        <  8:253772/138‑1 (MQ=255)

                        cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                      >  6:15737/1‑138 (MQ=255)

                        cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                                                                                      >  5:346230/1‑138 (MQ=255)

                        cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttta                                                                                                                      >  8:315026/1‑138 (MQ=255)

                        cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTGTTTATATGtttatttttt                                                                                                                     >  2:129624/1‑136 (MQ=255)

                          ttAGAAGTGCAAGTTAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                        <  5:202648/134‑1 (MQ=255)

                          ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCa                                                                                                                   >  2:273464/1‑139 (MQ=255)

                          ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                        <  2:302710/133‑1 (MQ=255)

                             gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATa                                                                                                                 <  1:350646/138‑1 (MQ=255)

                              aaGTGCAAGTGAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTATCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                                                                                                <  4:133130/138‑1 (MQ=255)

                                gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                             <  4:305391/139‑1 (MQ=255)

                                gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                             <  6:126744/139‑1 (MQ=255)

                                   caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                           >  5:66581/1‑138 (MQ=255)

                                   caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                                                           >  4:163665/1‑138 (MQ=255)

                                   caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGt                                                                                                          >  7:114013/1‑139 (MQ=255)

                                   caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                             >  4:396008/1‑135 (MQ=255)

                                   caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTa                                                                                                         >  5:378585/1‑139 (MQ=255)

                                              atatGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                                <  8:166198/138‑1 (MQ=255)

                                                 tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                                >  4:220380/1‑135 (MQ=255)

                                                 tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgc                                                                                            >  5:317535/1‑139 (MQ=255)

                                                 tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                           <  7:144070/139‑1 (MQ=255)

                                                   tatCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                           <  1:243585‑M2/135‑1 (MQ=255)

                                                   tatCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                           <  2:18389‑M2/135‑1 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATTAAGGAGTATTTGAAGAGTCGCGCgg                                                                                        >  1:183394‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                        >  2:215852‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                        >  7:234168‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                            <  8:286236‑M2/118‑1 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                        >  5:288329‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                        >  3:378855‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCTTCGCGCgg                                                                                        >  2:8943‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                                                                                       >  3:320548‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                                                                                       >  1:268707‑M2/2‑139 (MQ=255)

                                                     tCTATAATGATTTGTGGAAGTTTTGCCACAACAGCTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTg                                                                                                     <  6:241893‑M2/125‑1 (MQ=255)

                                                      cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                                <  5:41165/130‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAATGAGTATTTGAACCGTCGCGCGGGa                                                                                      >  2:157541/1‑137 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  1:3814/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  7:355123/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  4:247973/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  3:237494/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                     <  8:43453/138‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                     <  6:334739/138‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  3:177114/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  1:212280/137‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                     <  6:56993/138‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  6:213367/136‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      <  5:259839/136‑1 (MQ=255)

                                                         taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                                     >  3:119188/1‑137 (MQ=255)

                                                           aTGTTTTGTGGAAGTTTTGGGTCAACAGTTGG‑TTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACGGTCGCGCGGGACAGGtt                                                                                <  7:296976/139‑1 (MQ=255)

                                                              aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattt                                                                                                                        >  2:103510/1‑98 (MQ=255)

                                                               tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATTTCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGAGCGGGACAGGTTTa                                                                              >  2:362786/1‑139 (MQ=255)

                                                               tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                                                                              >  7:80601/1‑139 (MQ=255)

                                                               tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                                                                              >  1:157217/1‑139 (MQ=255)

                                                                ttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGTAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                             >  1:47425/1‑139 (MQ=255)

                                                                ttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTATTTATATCTTTATTTTATCATAAATGAGTATTTTAACAGTCGCGCGGGACAGGTTTAt                                                                             >  4:90828/1‑139 (MQ=255)

                                                                 tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                           >  8:195143/1‑124 (MQ=255)

                                                                 tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                                      >  5:11634/1‑129 (MQ=255)

                                                                 tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                             >  5:145816/1‑138 (MQ=255)

                                                                 tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                             >  1:7513/1‑138 (MQ=255)

                                                                 tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGACGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTAGCGCGGGACAGGTTTAt                                                                             >  8:373622/1‑138 (MQ=255)

                                                                   tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                           >  6:364097/1‑138 (MQ=255)

                                                                   tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                          <  1:128398/139‑1 (MQ=255)

                                                                   tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                          <  3:349161/139‑1 (MQ=255)

                                                                     gAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                        <  1:324261/139‑1 (MQ=255)

                                                                                  aacaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTAtt                                                            >  7:264785/1‑138 (MQ=255)

                                                                                        tGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACAGTAGCGGGGTACAGGTTTTTTATATGGATATTTTATTGaataaat                                                    >  3:207316/1‑137 (MQ=255)

                                                                                          gTTTATTTAC‑ATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  8:71807/138‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  4:276321/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATGATTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  3:146152/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  1:100046/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  2:33229/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  2:59826/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  7:326286/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  7:5356/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  3:260523/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  7:203436/139‑1 (MQ=255)

                                                                                           tttatttaCAATCTTATTTTTATTAGCCGTCAAATTTCACCCTCATCTCGTCTCTTTCTATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGTCAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  4:64958/139‑1 (MQ=255)

                                                                                            ttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  5:176102/138‑1 (MQ=255)

                                                                                            ttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  5:95825/138‑1 (MQ=255)

                                                                                            ttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTGTATGATAGGGATATTTTATTGAATAATTGAt                                                 >  6:83323/1‑139 (MQ=255)

                                                                                              atttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg                                                <  8:111117/138‑1 (MQ=255)

                                                                                              atttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                                                             >  3:289723/1‑93 (MQ=255)

                                                                                               tttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAAtt                                                    <  6:304719/133‑1 (MQ=255)

                                                                                               tttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCCCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATgg                                               >  3:76782/1‑138 (MQ=255)

                                                                                                 taCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGta                                             <  4:335542/138‑1 (MQ=255)

                                                                                                    aaTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTATCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGCCAGGTTTATGATTGGGATATTTTATTGAATAATTGATGGTATAAg                                         >  6:274225/1‑139 (MQ=255)

                                                                                                     aatctttttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTTAATAATTGATGGTATAAgg                                        >  2:244111/5‑139 (MQ=255)

                                                                                                     aTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGTCAGGTTTATTATATGGATATTTTATTGAATAATTGATGTTATAAgg                                        >  7:384805/1‑139 (MQ=255)

                                                                                                              ttattaGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTCTCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCa                                <  4:364141/138‑1 (MQ=255)

                                                                                                                attaGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGc                             >  4:192302/1‑139 (MQ=255)

                                                                                                                                  ctcTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                                  <  8:343669/98‑1 (MQ=255)

                                                                                                                                   ccTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTa            <  2:391359/137‑1 (MQ=255)

                                                                                                                                       aTCTCGTCTCTTTTTATATTTATTTTATCATAAAGGAGTATTTGAAACGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGATTAATTGATGGTATAAGGGACTTTCATGCTTGGAATGTGGGTATTATGatt       >  4:105166/1‑136 (MQ=255)

TAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGAT  >  pSIJ663_pHT315_araE_xy/2075‑2352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`