BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	8.8%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	8.8%	26565.5 / inf	9239	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (461/7853); new base T (883/37); total (1347/7892)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9437 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3597‑3877

   aaaaTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTaaa                                                                                                                                              >  8:313308/1‑139 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                          <  1:100321/139‑1 (MQ=255)

      aTCAGTTTCATCAAGCAATGAAACACGCCAAACTATACAATTTAAGTACCATTTCATATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAAATTg                                                                                                                                          <  5:147797/139‑1 (MQ=255)

       tCAGTTTCATCACGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                         <  4:283776/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                         <  7:365113/139‑1 (MQ=255)

        cattttCATCAAGTAATGAAAAACGCCAAAGTAAACAATTTAATTAACATTACTTATGAGCAAGTATTGTTTATTTTTAATTTTTATCTATTATTAAACTGGAGGAAATAATTCTATGAGTCGT‑TTTTTTATATTTGGa                                                                                                                                        <  4:377781/136‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                        <  7:314897/138‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                        <  5:250098/138‑1 (MQ=255)

         aGTTTCATAACTCAATCAAACACTCCAAAGTAAAAAATTTAAGTACCATTAATTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTAAAATTTGGa                                                                                                                                        <  4:219011/138‑1 (MQ=255)

                tcaAGCATTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  1:370798/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  1:240983/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  8:251596/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  7:265079/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  7:13706/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  5:225261/139‑1 (MQ=255)

                tcaAGCAATCACACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                <  1:185662/139‑1 (MQ=255)

                 cacgCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTacac                                                                                                                               <  2:228580/136‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  3:243780/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                              <  6:265693/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                         <  2:7624/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                         <  3:291372/139‑1 (MQ=255)

                        tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAATTTAAACGtttca                                                                                                                         >  6:299828/1‑135 (MQ=255)

                        tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAATTTGTAAATTTTCACCTTAca                                                                                                                         >  2:327737/1‑137 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  5:380432/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  7:290380/139‑1 (MQ=255)

                                 ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                               <  4:15478/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  2:45311/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  3:312935/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  3:36999/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  1:157500/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  2:120522/139‑1 (MQ=255)

                                          aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATATTTAAAGTTAAACGGTACTAAAGGGTATGGAGAAAAat                                                                                                      >  8:76218/1‑139 (MQ=255)

                                            aaTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGCAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatta                                                                                                    <  6:12965/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:302883/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  6:81914/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:70490/139‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  8:198206/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAAGTTTCAAGTTACATAAAGGAATGGTGattaattaa                                                                                                   >  8:109697/1‑134 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTAATTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  2:262496/138‑1 (MQ=255)

                                                 aaGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  3:218107/138‑1 (MQ=255)

                                                  aGTACCTTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  7:110390/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  2:294730/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAATTTACACGTTAATAAAGGGAAAGGAGATAAATTATTAGATAtac                                                                                          >  1:319828/1‑139 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGTAAGTTACACGTTAAT‑AATGGAATGGAGATAAATTATtatatataa                                                                                          >  6:389161/1‑137 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTAAAGTTTCACGTTTCTAAAGGGATTGGAGATAAATTATtatatataa                                                                                          >  8:223046/1‑138 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATATAACGGGAGGAAATAATTCTATGAGTAGC‑TTTTTTAAATTTCGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  5:12167/138‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  8:375019/139‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  3:86907/139‑1 (MQ=255)

                                                             ttATGAGCAAGTATTGTCTATTTTTTATAGTTATCTATAATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACTCTTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGAc                                                                                   <  5:203633/139‑1 (MQ=255)

                                                                tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGc                                                                                <  1:29449/139‑1 (MQ=255)

                                                                  aGCTAGTATTTTCTATTTTTAATAGTTATCTATTTTTTAACGTGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCt                                                                               <  1:40122/138‑1 (MQ=255)

                                                                     acagaTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  1:9238/135‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  2:195618/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  7:19392/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  3:193000/139‑1 (MQ=255)

                                                                     aaGGATTGTCTATTTTTACTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:42475/139‑1 (MQ=255)

                                                                       gTATTTTCTATTTTTAATAGTTATCTATTATTTAAACGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  8:345466/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  5:326112/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATATTTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:128852/138‑1 (MQ=255)

                                                                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                      <  6:225791/139‑1 (MQ=255)

                                                                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                      <  3:343123/139‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTGTAAAATTGTTAAGTTTCCCGGTAATAAAAGGAAAGGGTAGAAATTTTTTGATATAATAATGACACCATTCaagcacca                                                                    >  6:164369/1‑133 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  1:345584/139‑1 (MQ=255)

                                                                               tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTAACGGTACGAAATGGTATTGAGATAAATTATTTTATTTAATAATGACAGCTTGCAAGAAGCTaa                                                                  >  3:395343/1‑138 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTATTTTTTTATTTTAAAGGTTA‑TAAATGGAATTTAGATAAAATATTTTATATAATAAATACATCATCCAAGAATCAAAatag                                                              >  2:369028/1‑139 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGGAAGTTACACCGTTCTAAACGGATTTGACTTATATTATTTGATATTCTAATGACAGCTTGCCAGCAGCTaaatat                                                              >  7:84604/1‑136 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAAATTAAACCTTAATAAATGGAATTGTGATAAATTATTATATATAATAAAGTCAGCTTCCAAAAACCTAAaaag                                                              >  8:285071/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:266773/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  3:143163/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:63889/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  8:4586/138‑1 (MQ=255)

                                                                                          gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTa                                                       <  7:392723/138‑1 (MQ=255)

                                                                                              tCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgcgc                                                   <  5:176300/138‑1 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                          <  4:89347/138‑1 (MQ=255)

                                                                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTATTTTTTATATTTTAACGATTCTAAATTGTATTGTGTTTAATAATTATTTATAATAAAGACAACATGCAAGAAGAGAAAGAGGTCCCAAGCGCCCACGGGGGAt                                        >  8:138956/1‑139 (MQ=255)

                                                                                                         aCGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAATTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCGAGGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                        <  6:326530/138‑1 (MQ=255)

                                                                                                                gaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggta                                <  4:186015‑M1/139‑6 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  4:121255‑M1/139‑8 (MQ=255)

                                                                                                                      atTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                          <  4:52603‑M1/139‑12 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAACGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:357157‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGTAATGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:59217‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:98745‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:16538‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:168807‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                       <  7:337369‑M1/136‑15 (MQ=255)

                                                                                                                            tGAGTAGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                       <  8:169709‑M1/136‑15 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  4:343686‑M1/138‑18 (MQ=255)

                                                                                                                             gAGGCGA‑TTTTTTAAATTTGGAAAGGTACACTTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggttacattgaaaaagg                    <  6:49900‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                   <  2:157545‑M1/138‑19 (MQ=255)

                                                                                                                              aGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  1:213736‑M1/139‑20 (MQ=255)

                                                                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                 <  3:59885‑M1/139‑21 (MQ=255)

                                                                                                                                      tttttAATTTTGCAATGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  7:209932‑M1/138‑26 (MQ=255)

                                                                                                                                      tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATAGTACTGACAGCTTCAAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:267793‑M1/138‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  4:314682‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:354123‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  7:237299‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  6:189480‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:2761‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTCGAAAGATACACGTTAGTAAACTGAATGGATATAAATTATTAGATATACTACTGACAGATGCCAAGACGATAACGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGTGTAAATTGAAAAAGGAAgagtat       <  3:111636/137‑1 (MQ=255)

                                                                                                                                           aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  4:247023‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3597‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`