BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,741	G→T	9.4%	intergenic (+26/‑130)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,741	0	G	T	9.4%	27029.0 / inf	9271	intergenic (+26/‑130)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (266/7864); new base T (896/165); total (1206/8065)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9509 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3603‑3882

aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTg                                                                                                                                               <  1:100321/139‑1 (MQ=255)

aTCAGATTCCTCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTATAAATTTg                                                                                                                                               <  4:81013/139‑1 (MQ=255)

 tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTgg                                                                                                                                              <  7:206333/139‑1 (MQ=255)

   aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                             <  2:115109/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                       <  6:253619/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                       <  5:170685/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                       <  7:285968/138‑1 (MQ=255)

          tcaTGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  2:291907/139‑1 (MQ=255)

          tcaAGCGATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  8:42790/139‑1 (MQ=255)

          tcaAGCAATTAAACACGCCAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  8:195718/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:333970/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:287278/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:387746/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:54151/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  8:164464/139‑1 (MQ=255)

          tcaAGCAATCACACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  1:185662/139‑1 (MQ=255)

          tcaAGAAATGAACCACGACAAAGTAAACAATTTAAGTAACATTTCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:112845/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  4:370449/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:56002/139‑1 (MQ=255)

              gCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTGGAAAGTTACACg                                                                                                                                   <  3:338957/137‑1 (MQ=255)

               cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                             >  8:312107/1‑126 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                              <  2:7624/139‑1 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                              <  6:75639/139‑1 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                              <  1:293816/139‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATGATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  6:351496/139‑1 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  6:213555/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTaa                                                                                                                      <  4:308539/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  4:276745/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  3:168163/139‑1 (MQ=255)

                          gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAAATTTGATATTTTAACGTTAAGAAAGGGGAt                                                                                                                     >  6:287959/1‑139 (MQ=255)

                          gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGAAAATTACACCTTACGAAAGGGTAt                                                                                                                     >  7:78628/1‑139 (MQ=255)

                                gttaaCAATTTATGTACCATTACTTATGTGCAATTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGTGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                               <  4:112925/136‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  7:189507/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  1:319443/139‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  1:369713/138‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  4:178735/137‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:37282/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  8:34429/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:265935/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:232108/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  4:147536/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  3:142675/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTATTTTGTTATTTAAAAGGTTCTAAAAGGAAGTGTGAAAAattatt                                                                                                       >  6:46064/1‑139 (MQ=255)

                                        tttAAGTACCATTACGTATGAGCAAGTCATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTCTGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:359084/139‑1 (MQ=255)

                                        tttAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTATCGGGAGGAAAAAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  2:152767/139‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  2:36862/138‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  7:309986/138‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  5:61241/138‑1 (MQ=255)

                                           aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  5:91302/138‑1 (MQ=255)

                                            aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                   <  3:265280/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  1:157646/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  5:220560/139‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  2:259482/138‑1 (MQ=255)

                                                  aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTGGAAATTTACACGTTTATAAAGGGAATTGAGATAAAATATTATAtttaat                                                                                              >  1:21264/1‑136 (MQ=255)

                                                   ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGTAAGTGAGACGTTACTAAAGGGATTTGTGATAAATTATTATATTtactac                                                                                            >  8:75753/1‑139 (MQ=255)

                                                   ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTATATGAGTCGAGTTTTTTAATTTTTAAAATTAAAACTTATAAAAATGAAAGTATATAAATTATTATATTtaata                                                                                             >  8:373561/1‑138 (MQ=255)

                                                     gcTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                         <  3:391303/138‑1 (MQ=255)

                                                      cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                         <  5:114826/139‑1 (MQ=255)

                                                       ttATGTGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGACGAAATAAATCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATAGTACTGAc                                                                                        <  4:212088/139‑1 (MQ=255)

                                                       ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  6:317766/139‑1 (MQ=255)

                                                            aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTAACGGTAATTAAGGGGATGGAGATAAATTATTAGATATACTACAGAccggtc                                                                                   >  8:219081/1‑134 (MQ=255)

                                                               aaGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  5:321421/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  3:364793/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGGTAACTATTAATTAACGGGAGGATATAAATCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTAGTACAGGGTATGGAGATAAATTAATAGATATACTACTGACAGCTTCCa                                                                                <  5:196714/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTATAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  4:237821/139‑1 (MQ=255)

                                                                aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  6:182567/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  1:127754/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  5:399465/139‑1 (MQ=255)

                                                                    ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                           <  8:131153/139‑1 (MQ=255)

                                                                       tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                        <  3:81903/139‑1 (MQ=255)

                                                                         tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAAGTTAAACGGTTATAAAGGGAATTGTGATAAATTATTTGATATAATAATGAAAGCTTGCAAGAAGCTaaa                                                                      >  1:60954/1‑139 (MQ=255)

                                                                            ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTagagag                                                                   >  6:384296/1‑139 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  6:157188/138‑1 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  2:326076/138‑1 (MQ=255)

                                                                                            ttattTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACg                                                   <  2:44868/139‑1 (MQ=255)

                                                                                            ttattTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAATTTACACGTTACTAAAGGGAATGGAGATTAAATATTAGATATACTACTGACAGCATCCCAGAAGCTAAAGAGGTGCCAAGCGCCGACg                                                   >  8:283920/1‑139 (MQ=255)

                                                                                              attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  3:241357/138‑1 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAAGTTTAACGTTACTAAATGGAATTGAGATAAATTATTATATATAATAAATACAGCATCCAACAACCAAAAGAGGTCCCTAGCGCCTaacggg                                                >  7:161649/1‑134 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  5:347723/138‑1 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAAGTTTCACGGTACTAAAGGGTATGGAGATAAATTATTATATATACTAATGACAGCTTACAACAAGCTAAAGAGGTCCCGAGCGCCGACgggg                                                >  3:192312/1‑139 (MQ=255)

                                                                                                 tAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAAGTTAAACGTTACTAAAGGGAATTGAGATAAATTTTTATATATAATAAAGAAACCTTCCAAGAAGCTAAAGAGGTCCCTAACGCCTACGGGGaa                                              >  8:249496/1‑139 (MQ=255)

                                                                                                    cGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTGACCTTTAAAAAGGGAATGGTGATAAATTATTATATTTACTAAAGAAAGCATCCAAGAAGCTAAAGAGGTCCCGAGCGCCGACGGGGAAttt                                           >  4:337298/1‑139 (MQ=255)

                                                                                                              taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                 <  1:344803‑M1/139‑10 (MQ=255)

                                                                                                                atTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                               <  7:130575‑M1/139‑12 (MQ=255)

                                                                                                                      tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggttacattgaaaaagg                         <  3:309206‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  2:225664‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  7:106711‑M1/139‑18 (MQ=255)

                                                                                                                       gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  1:396808‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  5:141117‑M1/138‑18 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  3:216066‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  6:121130‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                        <  7:261480‑M1/138‑19 (MQ=255)

                                                                                                                        aGTCGCTTTTATAAATTTGG‑AAGGTACAAGTTACTAAAGGGACTGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  2:190530‑M1/138‑20 (MQ=255)

                                                                                                                         ttagattttttaaattTGGAAAGTTACACGTTAATAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  4:44416‑M1/133‑20 (MQ=255)

                                                                                                                              ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  5:271666‑M1/139‑26 (MQ=255)

                                                                                                                                ttttATATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  1:88370‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAATTTGGAAAGTTACACTGTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  5:296183‑M1/137‑26 (MQ=255)

                                                                                                                                    aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta            <  1:239943‑M1/138‑31 (MQ=255)

                                                                                                                                     aaTTTGGAAAGTTACACGTTACTACAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt          <  5:243833‑M1/139‑33 (MQ=255)

                                                                                                                                     aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt          <  8:103547‑M1/139‑33 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3603‑3882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`