BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,783	G→T	5.3%	intergenic (+68/‑88)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,783	0	G	T	5.3%	28499.8 / 208.5	8855	intergenic (+68/‑88)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base G (2479/5621); new base T (554/158); total (3051/5804)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9083 total aligned reads displayed.

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC  >  pSIJ663_pHT315_araE_xy/3646‑3924

 aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                             <  5:348147/139‑1 (MQ=255)

 aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                             <  8:317674/139‑1 (MQ=255)

  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                            <  8:47/139‑1 (MQ=255)

  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                                                            <  1:367935/139‑1 (MQ=255)

     ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                                                          <  7:140509/138‑1 (MQ=255)

     ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                                                          <  8:106732/138‑1 (MQ=255)

           cTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                                   <  8:221793/139‑1 (MQ=255)

           cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                                   <  8:137098/139‑1 (MQ=255)

            ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                                  <  4:234124/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  5:131757/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  3:80986/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  5:216881/139‑1 (MQ=255)

                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                          <  1:201643/139‑1 (MQ=255)

                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTACGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                        <  5:379362/139‑1 (MQ=255)

                           gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                                                                    <  4:32723/138‑1 (MQ=255)

                              tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                <  5:240535/139‑1 (MQ=255)

                              tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCACTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                                                                  >  7:233877/1‑137 (MQ=255)

                                tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                                                                <  3:282133/137‑1 (MQ=255)

                                  tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                             <  2:35591/138‑1 (MQ=255)

                                  tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                             <  7:288743/138‑1 (MQ=255)

                                          ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGACGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                             <  7:314269/130‑1 (MQ=255)

                                                 ttattTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTAc                                                                                              <  7:167604/138‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                            <  6:334065/138‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                            <  3:308457/138‑1 (MQ=255)

                                                   attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                            <  1:258666/138‑1 (MQ=255)

                                                    tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                           <  2:195814/138‑1 (MQ=255)

                                                       agagacaggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCCACGGGGAAt                                                                                       <  3:101317/133‑1 (MQ=255)

                                                            gaggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                  <  8:250821‑M1/139‑3 (MQ=255)

                                                            acagaaatAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                  <  7:365787‑M1/136‑3 (MQ=255)

                                                             aggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                 <  1:286701‑M1/139‑4 (MQ=255)

                                                              tgaaATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggt                                                                                <  2:226112‑M1/138‑5 (MQ=255)

                                                                 aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                             <  3:133285‑M1/139‑8 (MQ=255)

                                                                 aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                             <  8:1710‑M1/139‑8 (MQ=255)

                                                                 aataatTCTAGGAGGCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAACGGCAATGCAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                             <  2:66943‑M1/139‑8 (MQ=255)

                                                                   taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                           <  2:156440‑M1/139‑10 (MQ=255)

                                                                           ttagtCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  2:286724‑M1/137‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  7:390546‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  5:100611‑M1/139‑18 (MQ=255)

                                                                           tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  8:378175‑M1/139‑18 (MQ=255)

                                                                            tagTCTCTTTTTTAAATTTGGAAAGTAACAAGTTACTAACGGGTATGCATATAAATTATTAGATATACAACTGACAGCTTCCAAGAAGCTATATAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  5:18921‑M1/137‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGTAAAGTTACAGGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:155774‑M1/138‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  4:267092‑M1/138‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGTATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  5:404572‑M1/138‑18 (MQ=255)

                                                                            gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  1:340861‑M1/138‑18 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                 <  3:258003‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGCAGATAAATTATAAGATATACTACTGACAGCTACCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                 <  4:17536‑M1/139‑20 (MQ=255)

                                                                             aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGCGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                 <  6:89111‑M1/139‑20 (MQ=255)

                                                                                cGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                                                              <  6:366952‑M1/139‑23 (MQ=255)

                                                                                   ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  8:199622‑M1/139‑26 (MQ=255)

                                                                                    tttttAATTTTGCAATGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  7:209932‑M1/138‑26 (MQ=255)

                                                                                    tttttAAATTTGGAAATTTACACGTTACTAATGGGAATGGAGATAAATTATTTGATATACTACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  5:267366‑M1/138‑26 (MQ=255)

                                                                                    tttttAAATTTGGAAAGTTACACGTTAATAAAGTGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  1:80835‑M1/138‑26 (MQ=255)

                                                                                     ttttAAATTTTGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  5:162575‑M1/137‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  4:389290‑M1/137‑26 (MQ=255)

                                                                                     ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  5:68886‑M1/137‑26 (MQ=255)

                                                                                      tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                                                         <  1:53004‑M1/138‑28 (MQ=255)

                                                                                         aaaTTTGGAAAGTTCCACGTTACTAATGGGATTGGAGATAATTTATTAGATTTACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                      <  6:302190‑M1/138‑31 (MQ=255)

                                                                                          attttgtAAAGTTAGACGTTTCTAATGTGCATGGATATAAATTATTAGATATACTAATGACAGATTCCAAGACGCTAAAGAGGTCCCTAGCGCCTTCGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                    <  1:228841‑M1/137‑33 (MQ=255)

                                                                                          aaTTTGGATAGTTACACGTTTCTAATGTGAATGGAGATAAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGTGGTCCCTAGCGCCTTCGGGGAATTTGgggtactttgaaaaaggaagagtatgagtatt                                                    <  8:284490‑M1/139‑33 (MQ=255)

                                                                                          aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                    <  2:321354‑M1/139‑33 (MQ=255)

                                                                                              tGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa                                                 <  3:161408‑M1/138‑36 (MQ=255)

                                                                                                        ttcgtTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAACCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  4:115457‑M1/137‑47 (MQ=255)

                                                                                                              aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc                                 <  1:101564‑M1/138‑52 (MQ=255)

                                                                                                              aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           >  2:393938‑M1/1‑87 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                >  3:340776‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  8:76873‑M1/132‑47 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      >  4:210581‑M1/1‑86 (MQ=255)

                                                                                                               cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                                                                  >  5:318735‑M1/1‑86 (MQ=255)

                                                                                                                   aGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattca                                                  >  2:262163‑M1/1‑82 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  1:316111‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  3:368223‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                       >  7:113172‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      >  1:1254‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      >  1:388978‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  5:291164‑M1/103‑26 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           >  4:356325‑M1/1‑78 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                           <  7:26209‑M1/103‑26 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                 <  4:60197‑M1/97‑20 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  3:53383‑M1/95‑18 (MQ=255)

                                                                                                                       aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   >  4:143479‑M1/1‑78 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  6:274733‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  3:390135‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  7:190468‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  5:97247‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                     >  6:13600‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgacgttattcactttt                     >  3:212082‑M1/1‑76 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                                                         <  6:200970‑M1/103‑28 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  7:53785‑M1/93‑18 (MQ=255)

                                                                                                                         tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑ATTTGgggttcattgaaaaaggaagggtaagagtattgaacatttccgtggcgcccttattgccgtttt                    >  4:298703‑M1/1‑75 (MQ=255)

                                                                                                                             gaTAAATAATTAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc                 <  3:186595‑M1/139‑68 (MQ=255)

                                                                                                                              aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   >  6:232609‑M1/1‑71 (MQ=255)

                                                                                                                              aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  7:8001‑M1/88‑18 (MQ=255)

                                                                                                                               tAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   <  2:113195‑M1/87‑18 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                      <  8:347951‑M1/114‑47 (MQ=255)

                                                                                                                                 aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                 <  7:338662‑M1/71‑4 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCCAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg        <  1:179999‑M1/139‑77 (MQ=255)

                                                                                                                                      ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg        >  6:64249‑M1/1‑63 (MQ=255)

                                                                                                                                      ttaGATATACTACTGAAAGCTTACAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgataaaggaagagtatgagtattcaccatttcattgtcgcccttattcccttttttgcgtcattttg        >  6:397973‑M1/1‑63 (MQ=255)

                                                                                                                                       taGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatg                                                          <  6:169645‑M1/88‑27 (MQ=255)

                                                                                                                                        aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                   >  3:235171‑M1/1‑61 (MQ=255)

AAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC  >  pSIJ663_pHT315_araE_xy/3646‑3924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`