BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,467	T→G	5.9%	intergenic (+736/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,467	0	T	G	5.9%	23308.4 / 259.9	10680	intergenic (+736/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (4666/5165); new base G (308/503); total (4990/5690)
Fisher's exact test for biased strand distribution p-value = 1.88e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 10755 total aligned reads displayed.

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAG  >  pSIJ663_pHT315_araE_xy/5329‑5604

gCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                            <  5:356549/139‑1 (MQ=255)

gCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                            <  4:320234/139‑1 (MQ=255)

   gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTAGTccg                                                                                                                                         >  4:293395/1‑137 (MQ=255)

   gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGGGATGCTCGTCAg                                                                                                                                         <  7:260677/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                        <  1:160966/139‑1 (MQ=255)

           tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgg                                                                                                                                 >  5:154396/1‑139 (MQ=255)

             cGGTAAGCGGCAGGGTCTGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTTACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                                <  4:112032/138‑1 (MQ=255)

             cGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCg                                                                                                                                  <  1:66627/136‑1 (MQ=255)

                tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCTTCAGGGGGGCGGAGcc                                                                                                                             >  3:336554/1‑138 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCGTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                          >  1:238124/1‑139 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCa                                                                                                                                          >  3:52179/1‑123 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                          >  4:185708/1‑139 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                          >  5:23413/1‑139 (MQ=255)

                          ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAAc                                                                                                                  >  1:271054/1‑139 (MQ=255)

                               gAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAg                                                                                                             >  2:118621/1‑139 (MQ=255)

                                 aCAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGc                                                                                                            >  3:191767/1‑138 (MQ=255)

                                       gcgcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                     <  1:90577/137‑1 (MQ=255)

                                        agCGCACTCGGGAGCTTCCAGGGGGAAACGCGTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCGGACTTTCGGGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑c                                                                                                   <  8:71102/139‑1 (MQ=255)

                                         gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTa                                                                                                                           <  8:379825/115‑1 (MQ=255)

                                         gcgcACGAGGGAGCTTACAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑cc                                                                                                  >  7:60969/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCCTCAGGGGGGCGGAGCCTATGGAAAAACGCCACCAACCCGG‑ccg                                                                                                 >  2:67013/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTGAAGGGGGCGGAGCCTATGGAAAAACGCCACCCACGCGG‑CCt                                                                                                 >  6:127666/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGcc                                                                                                               <  4:396046/126‑1 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                       <  8:346541/134‑1 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCt                                                                                                 >  7:283261/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCt                                                                                                 >  5:300376/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCt                                                                                                 >  8:212491/1‑139 (MQ=255)

                                             aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGAAGCTCGTAAGGGGGGCGGAGCCTATGGAAAAACGCCACCAACGCGG‑CCgttt                                                                                              >  2:15422/1‑139 (MQ=255)

                                               gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                                     >  1:302690/1‑115 (MQ=255)

                                               gAGGGAGATTACAGGGGGAAACGCCTGGTATCTTTATAGTCCTTTCGGGTTTCGCCACGTCTGAGTTGAGGGTCGTTTTTTGTGATGGTCGTCAGGGGGGCGGTGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTa                                                                                            <  2:24502/139‑1 (MQ=255)

                                                aGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCATATGTAAAAACGCCAGCAACGCGG‑CCTTTTTAc                                                                                           <  4:98751/139‑1 (MQ=255)

                                                  ggAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCCTCATGGGGGCGGAGCCTAAGGAAAAACGCCAGCAACGCGG‑CCTTTTTActg                                                                                         >  6:75364/1‑137 (MQ=255)

                                                    aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCTGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTGGTTAGGGGGGCGGTGCCGTTGGTAAAACCCCAGCAACCCGC‑GCGTTTTAAgggt                                                                                       >  5:333386/1‑137 (MQ=255)

                                                    aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTAGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCGTTTTTAGGtt                                                                                       >  7:342362/1‑139 (MQ=255)

                                                      cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGATTTTTGTTATTCTGGTCAGGGGGGGGGGGCCGATTGAAAAAACCCAGCACCGCGGACGTTTTTAGGGtac                                                                                      >  1:185773/1‑137 (MQ=255)

                                                           aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCctggcc                                                                                >  6:197331/1‑139 (MQ=255)

                                                             ggggAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCctggcctt                                                                              >  7:314256/1‑139 (MQ=255)

                                                                 aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                       <  3:171717/111‑1 (MQ=255)

                                                                   ccGCCGGGTATCTTTAAAGTGCTGCCGGGTTTCCACACCTCTTCCTTGAGCGAGTATTTTTGGGATCCTCGTCCGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCctggccttttgctg                                                                        <  3:79373/138‑1 (MQ=255)

                                                                   aCGGCTGCTATCTTTATAGTCCTGTCGGGTTTCGCCACCTAGGACTTGTGCGTCGTATTTTGTGATGGTCGTCAGGGGGGCGGCGCCTATGGAAAAACCCCCCCAAGGCGG‑CCGTTTTAGGGGTCctggccgttttcgg                                                                        >  8:264490/1‑135 (MQ=255)

                                                                        ggttttCTTTATGGTCCTGTCGGGTTCCGCCACCTCTGAGTTGTGGGTCGATTTTTGTGAGGCTCGTCTGGGGGGCGGTGCCTATGTAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCctggccttttgctggcctt                                                                   <  6:236108/134‑1 (MQ=255)

                                                                         gttttatttATTGTCCTGTGGTGTTTCTCGAGCTTTGAATTTCGCGTCGATTTTTGGGTTGTTCGTCTGGGGGGCGGTGCATATGGAAAAACGCCAGCAACGCGG‑CCTTTTTTCGGTTCctggccttttgctggcctt                                                                   <  4:256876/132‑1 (MQ=255)

                                                                           tATTTTTATAGTCCCGTCGGGATTCGCCACCTCTGACTTGAGGGTGGATTTTTGGGATGCTCGGCAGGGGGGCGGAGCCAATGTAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCctggccttttgctggccttttg                                                                <  1:333408/139‑1 (MQ=255)

                                                                              cTTTTTAGGCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGGCGATTTTTGGGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  5:126262/138‑1 (MQ=255)

                                                                              cTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  1:217202/138‑1 (MQ=255)

                                                                               tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGGTAGTCCGGGGGGCGGCGCCGATGGAAAAAAGCCCGCACCCCCG‑GCGTTTTACGGGTCCGgggcgtttgctgggcgattgctga                                                            >  5:46580/1‑137 (MQ=255)

                                                                               tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCAATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCcttgccgtttgctggccgttttagaa                                                            >  8:315230/1‑134 (MQ=255)

                                                                                 tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  7:187658/135‑1 (MQ=255)

                                                                                 tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  3:126561/135‑1 (MQ=255)

                                                                                 tataGTCCTGGCGGGTTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  3:289859/135‑1 (MQ=255)

                                                                                    aGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCa                                                                                                                                          <  2:42394/57‑1 (MQ=255)

                                                                                     gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTa                                                                                            <  6:174094/101‑1 (MQ=255)

                                                                                     gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTcc                                                                                     >  2:95583/1‑108 (MQ=255)

                                                                                     gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              >  5:198992/1‑131 (MQ=255)

                                                                                      tCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTg                                                      <  2:283123‑M1/138‑2 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   >  7:86122‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTGTCGTCATGGGGGCGGAGCCTATTGAAAAACGCCAGCACAGCGG‑GCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTTCTCACAAGTgcgg                                                   >  3:313061‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                                   >  6:277335‑M1/1‑135 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgg                                                >  6:101397‑M1/1‑132 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgg                                                >  6:152664‑M1/1‑132 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcg                                                 <  2:221940‑M1/138‑7 (MQ=255)

                                                                                           tCGGGTTTAGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCTTCAGGGGTGCGGCTCCTATTGAAAAACGCCACCAACGCGG‑CCATTTTAAGGTTCCTGGCCTTTTGCTTGCCGTTTGCTCACAAGTtcggcgg                                                >  7:397552‑M1/1‑132 (MQ=255)

                                                                                                tttCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCCACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggctt                                           >  5:175566‑M1/1‑127 (MQ=255)

                                                                                                tttCGCCACCTCTGACTTGAGCGTCGATTTTTTTGTTTGTAGTCAGGGGGGCGGAGCCTATGGAAAAACCCCAGCAACGCCG‑CCGTTTTACCGTTACTGGCCTTTTGGTGGCCTTTTGCTCACAAGTgcggcggggctg                                           >  8:143585‑M1/1‑127 (MQ=255)

                                                                                                   cGCCACCTCTTACTTGAGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTTTGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                         <  1:129207‑M1/138‑15 (MQ=255)

                                                                                                   cGCCAACTCTGACTTGAGCGTGGATTTTTGGGATGCTCGTCAGGGGGGAGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                         <  5:380226‑M1/138‑15 (MQ=255)

                                                                                                    gCCACCTCTCACTTGAGGGTCGATTTTTGTGCTGCGCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcg                                       <  7:215026‑M1/139‑17 (MQ=255)

                                                                                                    gCCACCGCTTACTTGAGCGGCGTTTTTTGTGCTGGGGGTGGGGGGGGGGGAGCCTATGGAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcg                                       <  2:194937‑M1/139‑17 (MQ=255)

                                                                                                     ccACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  6:312515/115‑1 (MQ=255)

                                                                                                       aCCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGTAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                                    <  3:147775‑M1/139‑20 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  8:236545‑M1/139‑22 (MQ=255)

                                                                                                         ctctGACATGAGCGTCGATTTTGGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                                  <  6:370121‑M1/139‑22 (MQ=255)

                                                                                                             gACTTGAGCGGCGATTTTTGTGATGGGCGTCAGGGGGGCGGAGCCTATGCAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  2:353457‑M1/139‑26 (MQ=255)

                                                                                                              cctTGCGGGTCGATTTTTGTGTTGCTCGTCAGGGGGGGGGAGCCTATGGAAACACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  5:266483‑M1/137‑26 (MQ=255)

                                                                                                              cctTGAGCGTGGAGTTTTGTGATGGTCGTGAGGGTGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  1:100583‑M1/137‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAATCGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                             <  8:28734‑M1/139‑27 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                             <  3:258704‑M1/139‑27 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                             <  8:48648‑M1/139‑27 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  3:227959‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  1:44466‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              <  4:217862‑M1/138‑26 (MQ=255)

                                                                                                               cTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                             <  3:364659‑M1/138‑27 (MQ=255)

                                                                                                                  gAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTAc                                                                                           >  7:124844/1‑73 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggc                         <  3:387452‑M1/138‑31 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggc                         <  4:138237‑M1/138‑31 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                              >  4:64968‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                          <  7:23578‑M1/121‑14 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                          >  7:100270‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG                                                        >  5:76238‑M1/1‑107 (MQ=255)

                                                                                                                    gcggcgATTTTTGGGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggcta                       <  8:178682‑M1/139‑33 (MQ=255)

                                                                                                                        cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTggccttttgctggccttttgct                                                              <  4:371754/96‑1 (MQ=255)

                                                                                                                            ttgagtgatgcTCGTCCGGGGGGCGGAGCGTATGCAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACTTGTgcggcggggcttggcggcttattgtatggctatgacacg                <  1:369449‑M1/134‑40 (MQ=255)

                                                                                                                               tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggca             <  8:10940‑M1/138‑43 (MQ=255)

                                                                                                                               tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggca             <  6:344783‑M1/138‑43 (MQ=255)

                                                                                                                                 tgATGCTCGTAAGGGGTGAGGAGCCTTTGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCGTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggaggggcttggcggcttattgtatggctatgacacggcagt           <  6:399801‑M1/138‑45 (MQ=255)

                                                                                                                                 tgATGCTCGGCCGGGGGGCGGAGCCTTTGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggcagtg          <  7:154517‑M1/139‑46 (MQ=255)

                                                                                                                                     gCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggcagtgattt      >  2:3167‑M1/1‑90 (MQ=255)

                                                                                                                                        cGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacacggcagtgatttctg   >  4:348913‑M1/1‑87 (MQ=255)

                                                                                                                                         gTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTa                                                                                            >  7:369035/1‑49 (MQ=255)

                                                                                                                                         gTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgag  <  4:161223/139‑1 (MQ=255)

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG‑CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAG  >  pSIJ663_pHT315_araE_xy/5329‑5604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`