Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	999,371	C→G	7.0%	L262V (CTC→GTC)	U712_04880 →	UPF0053 protein yhdT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	999,371	0	C	G	7.0%	195.6 / 6.5	57	L262V (CTC→GTC)	U712_04880	UPF0053 protein yhdT
Reads supporting (aligned to +/- strand):  ref base C (30/23);  new base G (2/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CAAAGAAATTATGATACCGCGGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTG  >  CP006881/999238‑999505                                                                                                                                      |                                                                                                                                       cAAAGAAATTATGATACCGCGGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGt                                                                                                                                   <  4:52623/139‑1 (MQ=255) cAAAGAAATTATGATACCGCGGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGt                                                                                                                                   <  1:242009/139‑1 (MQ=255) cAAAGAAATTATGATACCGCGGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGt                                                                                                                                   <  3:171490/139‑1 (MQ=255)                  cgcgGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCa                                                                                                                  >  8:27865/1‑139 (MQ=255)                         ggaaATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGttt                                                                                                           >  6:84737/1‑139 (MQ=255)                                gctgcgTTGGCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGAtt                                                                                                    <  4:59824/139‑1 (MQ=255)                                    ctgTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCAGAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTg                                                                                                  <  7:27865/137‑1 (MQ=255)                                    ctgTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTg                                                                                                  <  3:178359/137‑1 (MQ=255)                                       tgtCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGtt                                                                                             >  6:385366/1‑139 (MQ=255)                                       tgtCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGtt                                                                                             >  2:234088/1‑139 (MQ=255)                                                 gaTGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACag                                                                                     >  4:150914/1‑137 (MQ=255)                                                     aagATGGACGATATGCTTGTAGTCATGCTGATGGAAAAATACTCGCGCTGGCCAGTCACAGATGGAGACAAAGACAGGTTGCTCGGTTTGGTGAATAGGAAGCATTTGTTTTCTGATTTGGTGTTTATTACagaagaag                                                                               <  7:263653/137‑1 (MQ=255)                                                          ggCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTg                                                                                                  <  1:213461/115‑1 (MQ=255)                                                          ggCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTg                                                                                                  >  2:213461/1‑115 (MQ=255)                                                          ggCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACg                                                                           >  6:404469/1‑138 (MQ=255)                                                               agaTGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGGTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATg                                                                       >  5:321130/1‑137 (MQ=255)                                                               agaTGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGGTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATg                                                                       >  5:315304/1‑137 (MQ=255)                                                               agaTGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATg                                                                       >  7:238382/1‑137 (MQ=255)                                                                        aaGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  5:385366/139‑1 (MQ=255)                                                                        aaGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  6:360899/139‑1 (MQ=255)                                                                         aGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  4:946/138‑1 (MQ=255)                                                                         aGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  1:234088/138‑1 (MQ=255)                                                                             aTGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACATAAGAAGAACGAATGAAGATGACGatccatc                                                       >  7:102769/1‑139 (MQ=255)                                                                             aTGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGatccatc                                                       >  5:329217/1‑139 (MQ=255)                                                                              tGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGatccatcc                                                      >  3:40514/1‑139 (MQ=255)                                                                              tGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGatccatcc                                                      >  4:245347/1‑139 (MQ=255)                                                                              tGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  7:271424/133‑1 (MQ=255)                                                                              tGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            >  8:271424/1‑133 (MQ=255)                                                                                   aaGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTAtg                                                 >  7:207171/1‑139 (MQ=255)                                                                                     ggAACAATACACGCGCAGGCCAGTCACAGATGGAGACAAAGTCAGTGTGGTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTAtgtg                                               <  6:321130/139‑1 (MQ=255)                                                                                     ggAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGGTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTAtgtg                                               <  6:315304/139‑1 (MQ=255)                                                                                     ggAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTAtgtg                                               <  8:238382/139‑1 (MQ=255)                                                                                             acacGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGt                                       >  2:213676/1‑139 (MQ=255)                                                                                              cacGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGt                                       <  3:245347/138‑1 (MQ=255)                                                                                                 gcgcTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATc                                   >  7:187201/1‑139 (MQ=255)                                                                                                         cAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGAt                           <  6:329217/139‑1 (MQ=255)                                                                                                                     gagaCAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGcc                                          >  1:209976/1‑112 (MQ=255)                                                                                                                     gagaCAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGcc                                          <  2:209976/112‑1 (MQ=255)                                                                                                                     gagaCAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATcc                <  3:150914/138‑1 (MQ=255)                                                                                                                          aaaGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGtt           >  2:185458/1‑138 (MQ=255)                                                                                                                            aGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCAt        >  3:51251/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGa                                                                <  8:84525/77‑1 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGa                                                                >  7:84525/1‑77 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACg                                                              >  5:190620/1‑79 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACg                                                              <  6:190620/79‑1 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            <  2:308093/81‑1 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGat                                                            >  1:308093/1‑81 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCg                                         <  2:132673/100‑1 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCg                                         >  1:132673/1‑100 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGaa                                <  3:38515/109‑1 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGaa                                >  4:38515/1‑109 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg  >  2:51538/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg  >  3:23491/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg  >  1:396925/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg  >  8:24450/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTg  >  5:403575/1‑139 (MQ=255)                                                                                                                                  tgtgCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAAAGATCCCGGTTCATGACTTg  >  2:320782/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       CAAAGAAATTATGATACCGCGGAAGGAAATGGCTGCTGTGTCAACTGAGATGACGATGGCCGAGATGCTTGAAGTCATGCTGAAGGAAAAATACACGCGCTGGCCAGTCACAGATGGAGACAAAGACAGTGTGCTCGGTTTGGTCAATACGAAGCATTTGTTTTCTGATTTGCTGTTTATGACAGAAGAAGAACGAATGAAGATGACGATCCATCCGTATGTGAGGCCGGTCATCGAAGTGATTGAAACGATCCCGGTTCATGACTTG  >  CP006881/999238‑999505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑