Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,046,124 G→A 7.4% A44V (GCG→GTG)  U712_05140 ← Uncharacterized protein yhgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,046,1240GA 7.4% 166.9 / 2.5 54A44V (GCG→GTG) U712_05140Uncharacterized protein yhgB
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base A (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

TTGGGAAGCATTCAGGAGAAAAAATGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCAATAAAATAAGCTGCCGGACATGCCGGC  >  CP006881/1045987‑1046260
                                                                                                                                         |                                                                                                                                        
ttGGGAAGCATTCAGGAGAAAAAATGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCg                                                                                                                                          >  3:168040/1‑138 (MQ=255)
        cATTCAGGAGAAAAAATGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACggg                                                                                                                                 >  4:133752/1‑139 (MQ=255)
          ttCAGGAGAAAAAATGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACggg                                                                                                                                 >  4:38588/1‑137 (MQ=255)
                  aaaaaaTGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGATCTGAAAACCGCAGCACGGGCAGATTTCAt                                                                                                                       >  1:190617/1‑139 (MQ=255)
                  aaaaaaTGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCAt                                                                                                                       >  3:32497/1‑139 (MQ=255)
                    aaaaTTACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGGGCTGCATCTCGTACTCATCAAATCCGAACTTAAAACCGCAGCACGGGCAGATTTCATga                                                                                                                     <  2:135382/139‑1 (MQ=255)
                    aaaaTGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATga                                                                                                                     <  3:115880/139‑1 (MQ=255)
                             cACCCTTCCGCAAGCCATTTTTTTCTATAGGCGATGAGGCTTTCCGAGGGCTCTATATGAGTTAGCCTCGTGCTGCTTCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTg                                                                                                            <  4:168040/139‑1 (MQ=255)
                                  ttCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCtt                                                                                                        >  6:385540/1‑138 (MQ=255)
                                          gCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCa                                                                                                >  8:7506/1‑138 (MQ=255)
                                                   ttCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGt                                                                                       <  4:32497/138‑1 (MQ=255)
                                                          ggCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCa                                                                                <  8:293669/138‑1 (MQ=255)
                                                          ggCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCa                                                                                <  8:198126/138‑1 (MQ=255)
                                                                gatgCTTTCCGATGGCTCTATATGAGTTAGCCGCGTGCTGCAGCTCGTAATCATCAAATCCGACCTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         <  3:38588/139‑1 (MQ=255)
                                                                 ttgCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         <  6:46402/137‑1 (MQ=255)
                                                                          gATGGCTCTATATGAGTTATCCTCGTGCTTCATCTCTTACTCATCAAATCCGAACTGAACACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGa                                                               <  1:200492/139‑1 (MQ=255)
                                                                          gATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGa                                                               <  1:308533/139‑1 (MQ=255)
                                                                          gATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGa                                                               <  7:173272/139‑1 (MQ=255)
                                                                              gCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTg                                                            <  3:133752/138‑1 (MQ=255)
                                                                                         gTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCt                                                >  1:101734/1‑139 (MQ=255)
                                                                                         gTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCt                                                >  4:195555/1‑139 (MQ=255)
                                                                                          ttATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTggg                                                          >  8:160202/1‑128 (MQ=255)
                                                                                          ttATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTggg                                                          <  7:160202/128‑1 (MQ=255)
                                                                                           tATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCtt                                               >  1:109612/1‑138 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTTAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         <  6:371662/111‑1 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTTAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         >  5:371662/1‑111 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         >  7:348047/1‑111 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTa                                                                         <  8:348047/111‑1 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  8:86274/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  3:108667/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  3:197054/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  3:250052/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  3:260011/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  4:314617/1‑139 (MQ=255)
                                                                                            aTCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGAGTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCa                                             >  7:302375/1‑139 (MQ=255)
                                                                                                  gTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGc                                       >  7:200061/1‑139 (MQ=255)
                                                                                                        cATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATCTGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTtcct                                 <  8:368859/139‑1 (MQ=255)
                                                                                                        cATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTtcct                                 <  7:7506/139‑1 (MQ=255)
                                                                                                             cGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATTCTTCATTATGCTTTCCTCCCaa                            >  5:146751/1‑139 (MQ=255)
                                                                                                              gTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTtcctcc                               <  3:195555/135‑1 (MQ=255)
                                                                                                              gTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCaa                            <  4:260011/138‑1 (MQ=255)
                                                                                                                  tcatcaAATCCGAACTGAAAACCACAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCt                                                >  6:190959/1‑114 (MQ=255)
                                                                                                                  tcatcaAATCCGAACTGAAAACCACAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCt                                                <  5:190959/114‑1 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGa                                                               >  8:390138/1‑97 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGa                                                               <  7:390138/97‑1 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGt                                                     <  4:77349/107‑1 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCaataaaat                      >  6:148004/1‑138 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCaataaaat                      >  4:305003/1‑138 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCaataaaat                      >  6:148448/1‑138 (MQ=255)
                                                                                                                    atcaAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAAAGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGt                                                     >  3:77349/1‑107 (MQ=255)
                                                                                                                              aaCTGAAAACCACAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGc                                                         >  3:356342/1‑93 (MQ=255)
                                                                                                                              aaCTGAAAACCACAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGc                                                         <  4:356342/93‑1 (MQ=255)
                                                                                                                                   aaaaCCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCAATAAAATAAGCTGCCGGa           <  4:57248/134‑1 (MQ=255)
                                                                                                                                   aaaaCCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCAATAAAATAAGCTGCCGGa           >  3:57248/1‑134 (MQ=255)
                                                                                                                                       ccGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCAATAAAATAAGCTGCCGGACATGCCGGc  <  3:314617/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTGGGAAGCATTCAGGAGAAAAAATGACGCACCCTTCAGCAAGCCAGTTTTTTCTATAGGCGATGATGCTTTCCGATGGCTCTATATGAGTTATCCTCGTGCTGCATCTCGTACTCATCAAATCCGAACTGAAAACCGCAGCACGGGCAGATTTCATGAGAATGATTGCCTTCATGATCATATGGCGGTTCAGCCAATCCTTAAACCCGCAGACTGGGCAAGTATGCTTCATTTTGCTTTCCTCCCAATAAAATAAGCTGCCGGACATGCCGGC  >  CP006881/1045987‑1046260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑