Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,489,279 T→C 9.1% E170G (GAA→GGA)  U712_07650 ← Peptide deformylase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,489,2790TC 9.1% 115.0 / 4.7 44E170G (GAA→GGA) U712_07650Peptide deformylase 2
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base C (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.69e-01

CATACGCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCTTTTACTCTGATGC  >  CP006881/1489163‑1489411
                                                                                                                    |                                                                                                                                    
cATACGCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTAAGGGTGTTTGAAAGGATTTTACTTATCAATATGATAATAGa                                                                                                                 >  8:127861/1‑138 (MQ=255)
cATACGCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTATCTTTTAGCGCTCAATTGCGGTTGCATTTTGAGGTTCTTTGATATGCTTTTTCTTATCAATATTATCATAGaa                                                                                                                >  2:266365/1‑139 (MQ=255)
     gCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCCGCCGTTTTATCTTTTAGCGCGCAATTGGGATTGCATTTTCATGTTGTTTGTAAGGATTTTGGTTATGAATATGATCATATAACAtt                                                                                                            >  1:212102/1‑137 (MQ=255)
     gCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTGTGTTTTTGCGCTGAATTTGGATTGGATTTTGAGGGTGTTTGTAAGGATTTTTCTTTTAAATATGATGATAGAACATa                                                                                                            >  1:149780/1‑138 (MQ=255)
     gCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTGTCTTTTAGCGCTCAATTGGGATTGGATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATaa                                                                                                           >  3:110431/1‑139 (MQ=255)
     gCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTGTTGTCTTTTAGCGCTCAATTGGGATTGCATTTTCAGGTTGGTTGAAAGGATTTTGGGTATCAATATGATCATATGACATaa                                                                                                           >  1:220615/1‑139 (MQ=255)
         tCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTGTGTTTTTTCGCTCAATTTCGCTTGGATTTTTAGGGTATTTGAAATGATTTTCCTTATAAATATGATAATAGAACATatgggt                                                                                                       >  8:162702/1‑134 (MQ=255)
         tCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTATCGTTTAGAGCTCAATTGCGATTGCATTTTCAGGGTCTTTGAAAGGATTTTACTTATAAATATGATAATATAAAATAAcccc                                                                                                       >  8:109716/1‑139 (MQ=255)
                   gggCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCCGCCGGTTTGTGGTTTTGCGCGGAATTTCGCTTTGCTTTTGAGGTTCTTTTTAAGGTTTTTGATTTTCAATTTGATCATATAACAAAACGCCggtgaa                                                                                                 >  8:161734/1‑130 (MQ=255)
                   gggCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCCGCCGGTTTCTCTTTTAGCGCTCAATTGCGATTGGATTTTCAGGTTGTTTGTAATGATTTTCCGTATCAATATGATCATAGAACATAACGCCGGTCAAATga                                                                                             >  3:127595/1‑139 (MQ=255)
                   gggCCTTTTAAAATAAAGAAACGGCTAGCATTGTGCCAGCCGTTTTCTGTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATga                                                                                             >  3:36150/1‑139 (MQ=255)
                                                    tgcctgccGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATa                                                                                                                   <  4:340951/84‑1 (MQ=255)
                                                    tgCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATa                                                                                                                   <  8:20026/84‑1 (MQ=255)
                                                    tgCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATa                                                                                                                   >  7:20026/1‑84 (MQ=255)
                                                    tgCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCATTATCAATATGATCATa                                                                                                                   >  3:340951/1‑84 (MQ=255)
                                                         gCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCttt                                                       >  7:349467/1‑139 (MQ=255)
                                                         gCCGCTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTACTTATAAATATGATCATAGAAAATAACGCAGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATcaatt                                                       >  3:300844/1‑135 (MQ=255)
                                                             ttttCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTaaa                                                   <  7:181340/139‑1 (MQ=255)
                                                              tttCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTCCCTTATCAATATGATCATa                                                                                                                   <  2:131730/74‑1 (MQ=255)
                                                              tttCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTCCCTTATCAATATGATCATa                                                                                                                   <  2:125763/74‑1 (MQ=255)
                                                              tttCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTCCCTTATCAATATGATCATa                                                                                                                   >  1:125763/1‑74 (MQ=255)
                                                              tttATCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTCCCTTATCAATATGATCATa                                                                                                                   >  1:131730/1‑74 (MQ=255)
                                                                tctcTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACg                                                 <  2:27822/138‑1 (MQ=255)
                                                                tctcTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACg                                                 <  7:161734/138‑1 (MQ=255)
                                                                            cTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATAttt                                    >  2:209431/1‑139 (MQ=255)
                                                                            cTCAATTGCGATTGCATTTTCAGGGTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGGTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATAttt                                    <  8:306973/139‑1 (MQ=255)
                                                                               aaTTGCGATTGCATTTTCAGGTTCTTTGAAATGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTGCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCg                                 <  3:107596/139‑1 (MQ=255)
                                                                                    cGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTc                            <  4:246383/139‑1 (MQ=255)
                                                                                    cGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTc                            <  2:220615/139‑1 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCg                                 >  3:10108/1‑129 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCg                                 <  4:10108/129‑1 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTc                            >  2:310593/1‑134 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTc                            <  1:310593/134‑1 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAgtg                       >  1:154582/1‑139 (MQ=255)
                                                                                         gCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAgtg                       >  1:148917/1‑139 (MQ=255)
                                                                                              ttCAGGTTCTTTGTAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGtgttgt                   <  5:391739/138‑1 (MQ=255)
                                                                                              ttCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGtgttgt                   <  1:209431/138‑1 (MQ=255)
                                                                                                  ggTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCtt              <  5:330824/139‑1 (MQ=255)
                                                                                                  ggTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCtt              <  4:36150/139‑1 (MQ=255)
                                                                                                  ggTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCtt              <  4:110431/139‑1 (MQ=255)
                                                                                                  ggTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCtt              <  8:349467/139‑1 (MQ=255)
                                                                                                       tttGAAATGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCTTTTAct         <  4:300844/139‑1 (MQ=255)
                                                                                                         tGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCTTTTAct         <  2:149780/137‑1 (MQ=255)
                                                                                                              ggATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCTTTTACTCTGATGc  <  7:42632/139‑1 (MQ=255)
                                                                                                                    |                                                                                                                                    
CATACGCTGTCAAAGAATTGGGCCTTTTAAAATAAAGAAACGGCTGGCATTGTGCCAGCCGTTTTCTCTTTTAGCGCTCAATTGCGATTGCATTTTCAGGTTCTTTGAAAGGATTTTCCTTATCAATATGATCATAGAACATAACGCCGTTCAAATGATCGATCTCATGCTGAAACACAATAGCCGGAAATCCTTTTAAACGAATATCAATATTTTCGCCTTCTAGTGTTGTTCCTTTTACTCTGATGC  >  CP006881/1489163‑1489411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑