Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,642,306 T→A 9.8% V340D (GTT→GAT)  U712_08445 → Uncharacterized protein ylqG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,642,3060TA 9.8% 113.7 / 4.2 41V340D (GTT→GAT) U712_08445Uncharacterized protein ylqG
Reads supporting (aligned to +/- strand):  ref base T (10/26);  new base A (2/2);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

AAGCCCGCCAGCTGCTGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGTTCTTTCTTTCTTATCCAAAGTGAAAACGGCAATCGGCGCA  >  CP006881/1642174‑1642439
                                                                                                                                    |                                                                                                                                     
aagCCCGCCAGCTGCTGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACa                                                                                                                                 <  5:281293/139‑1 (MQ=255)
  gCCCGCCAGCTTCTGGTGAAGCTGACGGAATATGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAATTCACTTGCAGCTTCAGCTGTTTTACAgg                                                                                                                               <  1:210846/139‑1 (MQ=255)
  ccccGCCAGCGGCGGGTTAAGCGGACGGAATCTGCTGAAAAAAACAGTCTAAAAATTGTGAAAGAAGCAGCAAATTGGATTAACGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAgg                                                                                                                               <  5:64551/138‑1 (MQ=255)
       cccgctgctGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAATAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGca                                                                                                                          <  5:253513/136‑1 (MQ=255)
       ccAGCTGCTGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGca                                                                                                                          <  3:269547/139‑1 (MQ=255)
        cAGCTGCTGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGcac                                                                                                                         <  8:254196/139‑1 (MQ=255)
         aGCTGCTGGTGAAGCTGACGGAATCTGCTTAAAAAAACAGTCTCCAAATTGTGAAAGACGCAGCAAATTGGATTAACGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCTGcaca                                                                                                                        <  3:125079/139‑1 (MQ=255)
         aGCGGCTTGTGTAGTGGACGGAATCTGCTGAAAATAATAGTCTACATATTTTGAAATAATCAGAAAATTGGATTAAAGCGGCTGCTTCATCGGGTGTTAGTTAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGcaca                                                                                                                        <  4:283028/139‑1 (MQ=255)
             gctgGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGt                                                                                                                     >  4:348558/1‑138 (MQ=255)
             gctgGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGt                                                                                                                     >  4:159189/1‑138 (MQ=255)
              ctgGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGt                                                                                                                     <  1:115382/137‑1 (MQ=255)
              ctgGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTca                                                                                                                   >  4:58569/1‑139 (MQ=255)
              ctgGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTca                                                                                                                   <  1:19949/139‑1 (MQ=255)
                ggTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGca                                                                                                                          <  8:195560/130‑1 (MQ=255)
                ggTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGca                                                                                                                          >  7:195560/1‑130 (MQ=255)
                           cGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCCTCGGGTGATAGTACGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGc                                                                                                      >  2:334375/1‑139 (MQ=255)
                                    ctgAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTc                                                                                               <  7:133268/137‑1 (MQ=255)
                                          aaaaCAGTCTCCAAATTGTGCAAGAAGCAGCAAATTGGATTCAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTaaa                                                                                       <  1:278789/139‑1 (MQ=255)
                                          aaaaCAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTaaa                                                                                       <  6:158912/139‑1 (MQ=255)
                                          aaaaCAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTaaa                                                                                       <  4:326101/139‑1 (MQ=255)
                                            aaCCGTCTCCAAATTGTGACAGAAGCAGCAAATTGGATTCAAGCGGCTGCTTCATCTGGTGATCGTCAGTCACTTGCAGCTTCAGCTGGTTTACAGGCGGCACAAGTCACAGATCCGGAAGACGCAGTCTTTTTAAAgg                                                                                     <  8:383366/139‑1 (MQ=255)
                                            aaCAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAgg                                                                                     <  6:113831/139‑1 (MQ=255)
                                                 tctcCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGtt                                                                                <  2:59899/139‑1 (MQ=255)
                                                     cAAATTGTGAACGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTca                                                                              <  7:77457/137‑1 (MQ=255)
                                                     cAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAg                                                                                                 <  7:48162/118‑1 (MQ=255)
                                                     cAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAg                                                                                                 >  8:48162/1‑118 (MQ=255)
                                                        atttggAAAGAAGCAGCAAATTTGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGa                                                                         <  1:182031/134‑1 (MQ=255)
                                                           gtgAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAg                                                                      >  8:54763/1‑139 (MQ=255)
                                                            tgAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAg                                                                      <  7:273350/138‑1 (MQ=255)
                                                             gAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCa                                                                    <  5:324556/139‑1 (MQ=255)
                                                                        caAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTTCAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTc                                                                                               >  4:391037/1‑101 (MQ=255)
                                                                        caAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTTCAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTc                                                                                               <  3:391037/101‑1 (MQ=255)
                                                                                          ctgctTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGATTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGttct                                       >  4:182485/1‑139 (MQ=255)
                                                                                          ctgctTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGATTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGttct                                       >  4:177147/1‑139 (MQ=255)
                                                                                             ctTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGttcttt                                     <  3:348558/138‑1 (MQ=255)
                                                                                             ctTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGttcttt                                     <  3:159189/138‑1 (MQ=255)
                                                                                                       tGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGTTCTTTCTTTCTTATc                           >  2:119640/1‑138 (MQ=255)
                                                                                                                     ttGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCt                                                                                   >  4:92616/1‑68 (MQ=255)
                                                                                                                     ttGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCt                                                                                   <  3:92616/68‑1 (MQ=255)
                                                                                                                               aGCTGATTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGTTCTTTCTTTCTTATCCAAAGTGAAAACGGCAATCGGCGCa  <  3:182485/139‑1 (MQ=255)
                                                                                                                               aGCTGATTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGTTCTTTCTTTCTTATCCAAAGTGAAAACGGCAATCGGCGCa  <  3:177147/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AAGCCCGCCAGCTGCTGGTGAAGCTGACGGAATCTGCTGAAAAAAACAGTCTCCAAATTGTGAAAGAAGCAGCAAATTGGATTAAAGCGGCTGCTTCATCGGGTGATAGTAAGTCACTTGCAGCTTCAGCTGTTTTACAGGCGGCACAAGTCACAGATCAGGAAGCCGAAGTCTTTTTAAAGGCTGTTCAGCAGACAGCACCCCATTTGGCGGATAAGGCAGATGTTCTTTCTTTCTTATCCAAAGTGAAAACGGCAATCGGCGCA  >  CP006881/1642174‑1642439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑