| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,663,209 | G→T | 55.0% | intergenic (+11/‑11) | U712_08550 → / → U712_08555 | FlaA locus uncharacterized protein ylxG/Flagellar basal‑body rod protein flgG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,663,209 | 0 | G | T | 55.0% | 2.0 / 74.4 | 58 | intergenic (+11/‑11) | U712_08550/U712_08555 | FlaA locus uncharacterized protein ylxG/Flagellar basal‑body rod protein flgG |
| Reads supporting (aligned to +/- strand): ref base G (13/13); new base T (14/18); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
CAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGA > CP006881/1663074‑1663347 | cAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCtggtgg < 8:18759/138‑1 (MQ=255)cAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTggggg > 8:314366/1‑138 (MQ=255)cAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTggggg < 5:288271/138‑1 (MQ=255)cAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa < 7:146825/139‑1 (MQ=255)cAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGa < 7:179965/139‑1 (MQ=255)cAAAAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa < 5:174242/139‑1 (MQ=255) aCAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAat < 8:275155/139‑1 (MQ=255) gATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAatata < 3:185955/139‑1 (MQ=255) aTAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa > 7:200132/1‑133 (MQ=255) aTAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa < 8:200132/133‑1 (MQ=255) gCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTagg > 7:277992/1‑137 (MQ=255) cacaGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGt < 6:229947/139‑1 (MQ=255) cacaGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGt < 4:151550/139‑1 (MQ=255) aaGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACt < 2:26272/138‑1 (MQ=255) ctctGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTgggg > 3:361194/1‑112 (MQ=255) ctctGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTgggg < 4:361194/112‑1 (MQ=255) ctctGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa < 5:318907/114‑1 (MQ=255) ctctGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa > 6:318907/1‑114 (MQ=255) ctctGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTAt > 2:312243/1‑139 (MQ=255) tGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCt > 8:115399/1‑139 (MQ=255) gTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTg > 3:190641/1‑139 (MQ=255) gTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTg > 5:364077/1‑139 (MQ=255) gTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTg > 2:110159/1‑139 (MQ=255) gTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTg > 7:301987/1‑139 (MQ=255) tttAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATTTATTATGTTACGTTCACTTTATTCTGGATGCAGCg > 2:373932/1‑139 (MQ=255) tttAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCg > 5:399332/1‑139 (MQ=255) tttAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCg > 3:209038/1‑139 (MQ=255) aGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTAt > 5:184269/1‑138 (MQ=255) aaaTTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGaaa < 1:56715/139‑1 (MQ=255) attattATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa < 2:403225/91‑1 (MQ=255) attattATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGa > 1:403225/1‑91 (MQ=255) attattATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGaaaa > 5:34478/1‑138 (MQ=255) gTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTcaaaca < 7:314366/138‑1 (MQ=255) tccTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTcaaaca > 7:185519/3‑137 (MQ=255) tgGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTa < 6:364077/139‑1 (MQ=255) gACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCTTCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTGACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAAt < 7:2198/138‑1 (MQ=255) cGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCgg > 8:105333/1‑138 (MQ=255) aGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAacaaca > 2:291782/1‑139 (MQ=255) cGAATGTCATGTCTGTTGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc < 8:301987/137‑1 (MQ=255) cGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc < 7:105333/137‑1 (MQ=255) cGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc < 8:185519/137‑1 (MQ=255) cGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc < 7:115399/137‑1 (MQ=255) cGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCaa > 8:59050/1‑139 (MQ=255) cGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCaa > 8:244385/1‑139 (MQ=255) atgtcatgtcTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGt < 4:209038/139‑1 (MQ=255) gtcatgtcTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTa > 1:278732/1‑138 (MQ=255) tcatgtcTGTGGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTaaa < 2:278732/139‑1 (MQ=255) atgtcTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATa < 6:34478/139‑1 (MQ=255) gtcTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACg < 1:152133/107‑1 (MQ=255) gtcTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACg > 2:152133/1‑107 (MQ=255) tgGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGt < 6:372634/127‑1 (MQ=255) tgGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGt > 5:372634/1‑127 (MQ=255) tgGGACAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGt < 1:110159/136‑1 (MQ=255) tgGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGt < 7:315594/136‑1 (MQ=255) cAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc < 5:269085/117‑1 (MQ=255) cAATCATCTAAATAAAAACATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGcc > 6:269085/1‑117 (MQ=255) cAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTACTCGGTAACAACATTGCCAACGTAAATACAGTAGGATTc > 4:13909/1‑138 (MQ=255) tcatcTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGAt < 2:186892/133‑1 (MQ=255) tcatcTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGAt > 1:186892/1‑133 (MQ=255) tcatcTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCaaa > 4:123706/1‑138 (MQ=255) aataaaAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAagag < 1:312243/136‑1 (MQ=255) aaaaCATCTGGTGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCgtgt < 6:184269/138‑1 (MQ=255) aTCTTGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCtt < 2:343506/139‑1 (MQ=255) ggggAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCttt < 7:59050/135‑1 (MQ=255) ggggAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCttt < 7:244385/135‑1 (MQ=255) gggAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGa < 8:277992/139‑1 (MQ=255) | CAACAGATAAAACAGGCACAGTAAGCTCTGTTAAACATTTTAAAGGAAATTATTATCTCGTTCTTGATGATGGGACCGAGATCAGTCCTGCGAATGTCATGTCTGTGGGACAATCATCTAAATAAAAACATCTGGGGGAATATATTATGTTACGTTCACTTTATTCTGGAATCAGCGGTATGAAAAACTTTCAAACAAAGCTTGACGTAATCGGTAACAACATTGCCAACGTAAATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGA > CP006881/1663074‑1663347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |