Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,289,100 G→T 100% P184T (CCG→ACG)  U712_11585 ← putative oxidoreductase yqjQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,289,1000GT100.0% 91.5 / NA 32P184T (CCG→ACG) U712_11585putative oxidoreductase yqjQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/18);  total (14/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCC  >  CP006881/2288966‑2289226
                                                                                                                                      |                                                                                                                              
gATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGcc                                                                                                                             <  8:157870/138‑1 (MQ=255)
      ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCgg                                                                                                                          <  1:242488/135‑1 (MQ=255)
      ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGtt                                                                                                                       >  7:152764/1‑138 (MQ=255)
      ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTg                                                                                                                      >  5:245644/1‑139 (MQ=255)
               cGTAAAAATTGCAGCTGTTATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACtgttgt                                                                                                              <  4:262158/138‑1 (MQ=255)
                                       aGCTGCCACGTCATCAGGATCAAGCCTCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGc                                                                                     >  7:203013/1‑139 (MQ=255)
                                       aGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGGCGTTATGAGCAATGGGAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATGAATGCCGGTTCCCGAGAGc                                                                                     >  2:165438/1‑139 (MQ=255)
                                       aGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAATTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATTCCGGTTACCGa                                                                                         >  1:295110/1‑135 (MQ=255)
                                                 tcatcaGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAATATAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCa                                                                           <  6:130832/139‑1 (MQ=255)
                                                       ggATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGt                                                                     <  6:161600/139‑1 (MQ=255)
                                                           cAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACAt                                                                                                         >  5:376055/1‑99 (MQ=255)
                                                           cAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTGGACTGTTGTCACAt                                                                                                         <  6:376055/99‑1 (MQ=255)
                                                            aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                <  4:127900/139‑1 (MQ=255)
                                                            aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                <  5:137298/139‑1 (MQ=255)
                                                            aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                <  6:245644/139‑1 (MQ=255)
                                                            aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                <  6:173097/139‑1 (MQ=255)
                                                             aGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                >  2:375446/1‑138 (MQ=255)
                                                                          cGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGc                                                  <  1:375446/139‑1 (MQ=255)
                                                                            aCATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGGGTAACCTAACACGGCAt                                                >  8:57871/1‑139 (MQ=255)
                                                                                     gCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGGGTAACCTAACACGGCATGTTTGGTcg                                       <  7:57871/139‑1 (MQ=255)
                                                                                     gCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTcg                                       <  8:276465/139‑1 (MQ=255)
                                                                                            ccccGCCTTTATCAGCCATGGAAAAAAAGTCCGTCGGAATCGTGCCCGGGTTGACTGGTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCAAACACGGCATGTTTGGTCGCGGAATa                                <  4:90203/139‑1 (MQ=255)
                                                                                            ccccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTACCACGGCATGTTTGGTCGCGGAATa                                <  2:339029/139‑1 (MQ=255)
                                                                                            ccccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCGGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTGCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa                                <  8:152764/139‑1 (MQ=255)
                                                                                                     tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTGTGAGTAACCTAACACGGCATGTTTGCTCGCGGAATACAGGCTAGa                       >  4:15996/1‑139 (MQ=255)
                                                                                                     tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCAACCGCAAATCGTTTGAGTAACCTAACACGGCATGGTTGGTCGCGGAATACAGGCTAGa                       >  7:89882/1‑139 (MQ=255)
                                                                                                           cAATGGAAAAAAAGTCCGTCTTAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGTTCTCGGAATACAGGCTAGACTTCgg                 >  3:306784/1‑139 (MQ=255)
                                                                                                           cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCgg                 >  6:315624/1‑139 (MQ=255)
                                                                                                           cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCgg                 >  2:264071/1‑139 (MQ=255)
                                                                                                            aaTGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAaca                                                      >  7:166702/1‑101 (MQ=255)
                                                                                                            aaTGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAaca                                                      <  8:166702/101‑1 (MQ=255)
                                                                                                                          ccGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTccc  <  5:315624/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
GATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCC  >  CP006881/2288966‑2289226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑