Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,373,546 G→A 7.4% L71L (CTG→CTA)  U712_12035 → Uncharacterized protein yqzG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,373,5460GA 7.4% 171.9 / 2.5 54L71L (CTG→CTA) U712_12035Uncharacterized protein yqzG
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base A (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CTCTTCTCGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTTATAGCCAAAAGGTC  >  CP006881/2373416‑2373678
                                                                                                                                  |                                                                                                                                    
ctctTCTCGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGTGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaac                                                                                                                               >  7:334993/1‑138 (MQ=255)
   ttctCGTCTTTGGCACCACAGCCGCTCACCCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGATcaaaca                                                                                                                              <  5:189888/136‑1 (MQ=255)
   ttctCGTCTTTGCCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACAAGTCAAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaaca                                                                                                                              <  8:189058/136‑1 (MQ=255)
     ctcGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaacaaa                                                                                                                            <  8:12910/136‑1 (MQ=255)
     ctcGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaacaaa                                                                                                                            <  7:61883/136‑1 (MQ=255)
     ctcGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaacaaa                                                                                                                            <  7:398501/136‑1 (MQ=255)
     ctcGGCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTcaaacaaa                                                                                                                            <  4:116485/136‑1 (MQ=255)
             tGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGAc                                                                                                                 >  7:132149/1‑139 (MQ=255)
                 accacAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTa                                                                                                             <  7:352861/139‑1 (MQ=255)
                           cTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTaaaagaa                                                                                                       >  6:185663/1‑135 (MQ=255)
                              aCGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATg                                                                                                 <  7:398867/138‑1 (MQ=255)
                                ccAGAAGAACCACCGCTTGTAACCGCCCGCCACATGTCCAAATGGCAAGAGATTGCTGAAAAAGAACCAAAAAAACGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAg                                                                                              <  5:52684/138‑1 (MQ=255)
                                  agaagaAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAg                                                                                              >  7:187262/1‑137 (MQ=255)
                                   gaagaaACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAg                                                                                           <  1:12592/139‑1 (MQ=255)
                                   caataaaCACCGCTGGTAACCGCCCGCCACATGTCCAAATCGGAAGAGATTGCTGAAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAg                                                                                           <  1:149116/135‑1 (MQ=255)
                                    aagaaACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGt                                                                                          >  2:383765/1‑139 (MQ=255)
                                          cacCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGc                                                                                     >  4:35986/1‑138 (MQ=255)
                                             cGCTTGTAACCGCCCGCCACATGTCCAAATGTGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATa                                                                                 <  4:171235/139‑1 (MQ=255)
                                              gCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATAc                                                                                >  7:202188/1‑139 (MQ=255)
                                                 tGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCAt                                                                             >  2:20083/1‑139 (MQ=255)
                                                  gTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTg                                                                                         <  6:390427/126‑1 (MQ=255)
                                                  gTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTg                                                                                         >  5:390427/1‑126 (MQ=255)
                                                     aCCGCCCGCTACATTTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTCCCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTaa                                                                         <  8:334993/139‑1 (MQ=255)
                                                         cccgccACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAActct                                                                     >  2:182933/1‑139 (MQ=255)
                                                         cccgccACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTATTcaaacaaa                                                                                                                            <  6:351426/84‑1 (MQ=255)
                                                         cccgccACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTATTcaaacaaa                                                                                                                            >  5:351426/1‑84 (MQ=255)
                                                               acaTGTCCAAATGGGAAGTGATTGCTGTAAAACAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATCGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGa                                                               <  2:365777/139‑1 (MQ=255)
                                                               acaTGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGa                                                               <  5:185663/139‑1 (MQ=255)
                                                               acaTGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGa                                                               <  8:132149/139‑1 (MQ=255)
                                                                    tCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTaa                                                                         >  7:12902/1‑124 (MQ=255)
                                                                    tCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTaa                                                                         <  8:12902/124‑1 (MQ=255)
                                                                        aaTGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAATAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCaaa                                                      <  8:387269/139‑1 (MQ=255)
                                                                        aaTGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCaa                                                                                   >  8:387200/1‑110 (MQ=255)
                                                                        aaTGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCaa                                                                                   <  7:387200/110‑1 (MQ=255)
                                                                        aaTGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCaa                                                       >  4:376808/1‑138 (MQ=255)
                                                                              aaGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAAtt                                                >  1:252664/1‑139 (MQ=255)
                                                                              aaGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTATTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAActc                                                                      <  8:7975/117‑1 (MQ=255)
                                                                              aaGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTATTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAActc                                                                      >  7:7975/1‑117 (MQ=255)
                                                                                   aTTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCg                                           >  7:361775/1‑139 (MQ=255)
                                                                                       cTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGtgtt                                       >  4:80601/1‑139 (MQ=255)
                                                                                        tGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGttttt                                      >  6:124459/1‑139 (MQ=255)
                                                                                                 agCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTtgtg                                   <  3:84029/133‑1 (MQ=255)
                                                                                                 agCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTtgtg                                   >  4:84029/1‑133 (MQ=255)
                                                                                                 agCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGAACGTAAAAGAAAAGATGAAGCAGTGAAGCaa                                                                                   >  2:378830/1‑85 (MQ=255)
                                                                                                 agCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGAACGTAAAAGAAAAGATGAAGCAGTGAAGCaa                                                                                   <  1:378830/85‑1 (MQ=255)
                                                                                                  gCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGa                                                                                                <  7:35308/71‑1 (MQ=255)
                                                                                                  gCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGa                                                                                                >  8:35308/1‑71 (MQ=255)
                                                                                                  gCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTGTTTGTGACGAttt                            >  8:188553/1‑139 (MQ=255)
                                                                                                                cccGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTta               <  8:202188/138‑1 (MQ=255)
                                                                                                                cccGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTta               <  1:20083/138‑1 (MQ=255)
                                                                                                                cccGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTta               >  5:178169/1‑138 (MQ=255)
                                                                                                                cccGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTta               >  5:314507/1‑138 (MQ=255)
                                                                                                                        cacaGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTTATAGCCaaaa      <  3:35986/139‑1 (MQ=255)
                                                                                                                             gTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGCCGCTTTCATTTGATACTTATAGCCAAAAGGTc  >  8:393969/1‑138 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CTCTTCTCGTCTTTGGCACCACAGCCGCTCACGCAGAAGAAACACCGCTTGTAACCGCCCGCCACATGTCCAAATGGGAAGAGATTGCTGTAAAAGAAGCAAAAAAAAGGTACCCGCTCGCACAGGTACTGTTCAAACAAAAAGTATGGGACCGTAAAAGAAAAGATGAAGCAGTGAAGCAATACCATTTAACTCTGAGGGAAGGGTCAAAAGAATTCGGCGTTTTTGTGACGATTTCATTTGATCCTTATAGCCAAAAGGTC  >  CP006881/2373416‑2373678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑