| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,398,082 | A→G | 6.3% | I144T (ATA→ACA) | U712_12210 ← | putative ABC transporter permease protein yqgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,398,082 | 0 | A | G | 6.3% | 183.6 / 4.0 | 63 | I144T (ATA→ACA) | U712_12210 | putative ABC transporter permease protein yqgH |
| Reads supporting (aligned to +/- strand): ref base A (36/23); new base G (2/2); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01 | |||||||||||
AAGCCATTGCATCAGCAGAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCAGAA > CP006881/2397945‑2398219 | aaGCCATTGCATCAGCAGAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTAt > 6:15212/1‑139 (MQ=255) gCCATTGCATCAGCAGAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATg > 7:379675/1‑138 (MQ=255) cATCAGCAGAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATa < 4:158428/139‑1 (MQ=255) cagAAATCGATGTTATCGTCGGCAGAATCAGGACGGAAACGACAATCGTTCCCGCCAATACACTGTGCCCTGTTCCGATCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 3:330618/139‑1 (MQ=255) cagAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 6:322057/139‑1 (MQ=255) cagAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 5:15212/139‑1 (MQ=255) cagAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 5:144909/139‑1 (MQ=255) cagAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 5:371580/139‑1 (MQ=255) aTCTATGTGATCGTCGGCAGAATCAGGCCGTAACGGACAATCGTTCCCGCAAATAAACTGGGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGAACCAATACTGTAAGACCTATGAATCCATAAACAACAGATg < 8:379675/139‑1 (MQ=255) tgtgATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCATTACTGTAAGACCTATGAATCCAGAAACAACAGATTGAAt > 8:363224/1‑138 (MQ=255) aaTCATGACGGAAAGGATAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATa < 8:37053/108‑1 (MQ=255) aaTCATGACGGAAAGGATAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATa > 7:37053/1‑108 (MQ=255) aaTCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATAc > 5:131249/1‑125 (MQ=255) aaTCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATAc < 6:131249/125‑1 (MQ=255) tCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCActtt > 7:269874/1‑139 (MQ=255) tgtgCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt < 5:349792/133‑1 (MQ=255) tgtgCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt > 6:349792/1‑133 (MQ=255) gtgCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt < 4:57142/132‑1 (MQ=255) gtgCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt > 3:57142/1‑132 (MQ=255) tgCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCac > 2:100048/1‑138 (MQ=255) ttCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac < 8:127437/67‑1 (MQ=255) ttCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAaacaac > 7:127437/1‑67 (MQ=255) ttCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGt > 6:127644/1‑139 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAAt < 8:43582/51‑1 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAAt > 7:43582/1‑51 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAACGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATa > 2:154886/1‑138 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCt > 3:111321/1‑85 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCt < 4:111321/85‑1 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt < 7:268245/119‑1 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAtt > 8:268245/1‑119 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATa > 1:104775/1‑138 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATa > 5:17458/1‑138 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATa > 1:326349/1‑138 (MQ=255) cTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTATCTTTCCCCAATTAGGGGCAATCTCTGTCATa > 5:42845/1‑138 (MQ=255) tttGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGTGGCAATCTCTGTCATAAAAATc > 2:362895/1‑139 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTGTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCAcc < 7:244752/80‑1 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTGTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCAcc < 7:250269/80‑1 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTGTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCAcc > 8:244752/1‑80 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTGTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCAcc > 8:250269/1‑80 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGAt < 6:184796/53‑1 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGAt > 5:184796/1‑53 (MQ=255) gggCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCTACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGTGCAATATCTGGCATAAAAATCGCACCTg > 3:208697/1‑138 (MQ=255) ggCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGc > 3:303597/1‑138 (MQ=255) ggCGATAAACGGCACCAATACTGAAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGc > 2:352242/1‑138 (MQ=255) aCGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGTCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAg < 6:17458/139‑1 (MQ=255) aCGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAg < 7:363224/139‑1 (MQ=255) cAATACTGAAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCa < 1:352242/139‑1 (MQ=255) cggTAAGACCAATGAATCCCTAACCAACAGCAGGCATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTACCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCCAGCGGCGCAGCGAt < 6:86237/137‑1 (MQ=255) gTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCCATGCCAAGCGGCGCAGCGATTa > 1:198615/1‑139 (MQ=255) gTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGAt > 4:235846/1‑137 (MQ=255) gTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGAt > 3:54134/1‑137 (MQ=255) gTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGAt > 2:80544/1‑137 (MQ=255) gTAAGACCTATGAATCCATAAACAACAGAGGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTCCAATGCACAGCGGCGCGGCGag > 5:301681/1‑136 (MQ=255) tAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAAATCGGGGCAATCTCTGTCATAAAAATCGCACCTCCAATGCAAAGCGGCGCAGCGAt > 6:36252/1‑136 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCTATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCa < 3:157227/129‑1 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTg < 1:142297/112‑1 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTg > 2:142297/1‑112 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCa > 4:157227/1‑129 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTagag > 7:76487/1‑139 (MQ=255) aGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTagag > 2:291143/1‑139 (MQ=255) caCCTATGAATCCTTAAACAAACCTTGTAATACCTACTAAAACCTCAATCACCGGCTGCAGCACTTTCTTTCCCAAATTCGGGGAAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGc < 2:326349/138‑1 (MQ=255) ccTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTagag > 2:251501/1‑136 (MQ=255) ccTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTagag > 2:48560/1‑136 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCTGCGCAGCGATTAGAGCaga > 8:279473/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 2:371012/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 1:361674/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 3:326361/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 1:293055/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 6:227769/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCaga > 5:202905/1‑137 (MQ=255) aTGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAACCGGCGCAGCGATTAGAGCagaa > 6:399426/1‑138 (MQ=255) | AAGCCATTGCATCAGCAGAAATCGATGTGATCGTCGGCAGAATCATGACGGAAAGGACAATCGTTCCCGCCAATAAACTGTGCCCTGTTCCGCTCGATTTGAACTGGGCGATAAACGGCACCAATACTGTAAGACCTATGAATCCATAAACAACAGATGGAATACCGACTAAAAGCTCAATCACCGGCTGCAGCACTTTCTTTCCCCAATTCGGGGCAATCTCTGTCATAAAAATCGCACCTGCAATGCCAAGCGGCGCAGCGATTAGAGCAGAA > CP006881/2397945‑2398219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |