Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,471,279	G→A	5.4%	I448I (ATC→ATT)	U712_12615 ←	Putative DNA recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,471,279	0	G	A	5.4%	241.7 / 2.3	74	I448I (ATC→ATT)	U712_12615	Putative DNA recombinase
Reads supporting (aligned to +/- strand):  ref base G (43/27);  new base A (2/2);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

AAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGAT  >  CP006881/2471150‑2471413                                                                                                                                  |                                                                                                                                        aaGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGtt                                                                                                                                 >  7:106769/1‑138 (MQ=255)  aGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTaa                                                                                                                               >  7:99517/1‑139 (MQ=255)       gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACaa                                                                                                                            <  1:294539/137‑1 (MQ=255)       gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAAtt                                                                                                                          >  1:166037/1‑139 (MQ=255)       gCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAAtt                                                                                                                          >  1:340632/1‑139 (MQ=255)              aTGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGtttttt                                                                                                                   >  4:299237/1‑139 (MQ=255)               tGCAGCGAGGTTTATTGAAAATGAGATTGTAATTACAAAAGGGGTGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGttttttt                                                                                                                  <  3:168931/139‑1 (MQ=255)                 cAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTa                                                                                                                 <  2:238419/138‑1 (MQ=255)                     gAGGTGTATTGAAAATGAGATTGTAATTACAACAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTc                                                                                                            <  2:363878/139‑1 (MQ=255)                     gAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTc                                                                                                            <  6:87472/139‑1 (MQ=255)                      aGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTc                                                                                                            >  6:54544/1‑138 (MQ=255)                               gAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGagttaa                                                                                                   <  8:99517/138‑1 (MQ=255)                               gAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGagttaa                                                                                                   <  1:238307/138‑1 (MQ=255)                                aaaaTGAGATTGTAATTACAAAAGGGGGGTGCATACCCCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGagttaagt                                                                                                 >  6:229721/1‑139 (MQ=255)                                   aTGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTAt                                                                                              >  6:79583/1‑139 (MQ=255)                                   aTGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTAt                                                                                              >  7:147086/1‑139 (MQ=255)                                     gagaTTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCt                                                                                            >  2:108920/1‑139 (MQ=255)                                              aTTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCgat                                                                                    <  8:147086/138‑1 (MQ=255)                                              aTTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCgat                                                                                    <  3:107203/138‑1 (MQ=255)                                                              gCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGa                                                                   <  8:106769/139‑1 (MQ=255)                                                                      cccTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGttttttt                                                                                                                  <  6:270067/84‑1 (MQ=255)                                                                      cccTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGttttttt                                                                                                                  >  5:270067/1‑84 (MQ=255)                                                                      cccTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGc‑t                                                             <  3:18140/136‑1 (MQ=255)                                                                      cccTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGc‑t                                                             >  4:18140/1‑136 (MQ=255)                                                                       ccTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCttt                                                         >  3:140027/1‑139 (MQ=255)                                                                       ccTCTTTAATACGTTACAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCttt                                                         >  4:153354/1‑139 (MQ=255)                                                                         tctTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCtttt                                                        >  2:85593/1‑138 (MQ=255)                                                                         tctTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCtttt                                                        >  5:57022/1‑138 (MQ=255)                                                                          ctTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCtttt                                                        >  7:268776/1‑137 (MQ=255)                                                                           tttAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATATTCAAGAGTTAAGTTATCTGAACCGAATGATTGATAATAGTCGATGGA‑TCtttt                                                        >  6:246039/1‑136 (MQ=255)                                                                           tttAATACGTTTAAATATATATGGTGTCAGAATCCACAATGGTAAATTATTTCACGATAAAGTTAACAATTGATTTTTTATCTTCAAGAGTAAAGATATATGCACCGATTGATTGAAAATAGTCTATGGC‑TCtttt                                                        >  6:225430/1‑136 (MQ=255)                                                                                tACGTTTCAATATATATGGTGTCAGAATCCACAATTGTAACTTCTTTCACGATAAAGTTAAAAATTGTTTTTTTATCTTAAAGATTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATTGA‑TCTTTTTAGAGAAt                                                >  6:27869/1‑139 (MQ=255)                                                                                  cGTTTCAATATATATGGTGTCAGAATCCACAATGGTACCTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTAAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCAttt                                              >  7:308694/1‑139 (MQ=255)                                                                                    tttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCAtttt                                             >  4:309602/1‑138 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTc                                            >  7:123190/1‑138 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTc                                            >  6:223321/1‑138 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCa                                           >  4:264685/1‑139 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCa                                           >  4:338523/1‑139 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCa                                           >  8:271054/1‑139 (MQ=255)                                                                                     ttCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑ACTTTTTAGAGCATTTTCa                                           >  1:272159/1‑139 (MQ=255)                                                                                            tatatGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGc                                    <  8:268776/139‑1 (MQ=255)                                                                                             atatGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGc‑t                                                             >  5:206512/1‑113 (MQ=255)                                                                                             atatGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGc‑t                                                             <  6:206512/113‑1 (MQ=255)                                                                                              tatGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTc                                  >  3:145019/1‑139 (MQ=255)                                                                                               atGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCgat                                                                                    >  2:169720/1‑89 (MQ=255)                                                                                               atGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCgat                                                                                    <  1:169720/89‑1 (MQ=255)                                                                                                  gtgtCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGc                                                  <  5:81904/119‑1 (MQ=255)                                                                                                  gtgtCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGc                                                  >  6:81904/1‑119 (MQ=255)                                                                                                  gtgtCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               <  5:79583/138‑1 (MQ=255)                                                                                                  gtgtCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               <  2:384381/138‑1 (MQ=255)                                                                                                    gtCAGAATCCACAATGGTAACTTCTTTCACAATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               >  6:1971/1‑136 (MQ=255)                                                                                                    gtCAGAATCCACAATGGTAACTTCTTTCACAATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               <  5:7701/136‑1 (MQ=255)                                                                                                    gtCAGAATCCACAATGGTAACTTCTTTCACAATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               <  5:1971/136‑1 (MQ=255)                                                                                                    gtCAGAATCCACAATGGTAACTTCTTTCACAATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTa                               >  6:7701/1‑136 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGa                                                                   >  6:134694/1‑100 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGa                                                                   <  5:134694/100‑1 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGCATATTTTTTGCGATTTTACACTTGAGATctaata                             >  2:113245/1‑133 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTaga                                                    <  6:369751/114‑1 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTaga                                                    >  5:369751/1‑114 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACtt                                        >  1:217328/1‑126 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACtt                                        <  2:217328/126‑1 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTatcat                           >  5:158139/1‑139 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTatcat                           >  1:318590/1‑139 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTatcat                           >  3:346941/1‑139 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTatcat                           >  7:84194/1‑139 (MQ=255)                                                                                                     tCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTT‑GAGCATTTTAACTTGAGCTCGTatcatg                          >  2:364487/1‑138 (MQ=255)                                                                                                              acaATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACtt                  <  2:340632/139‑1 (MQ=255)                                                                                                                     tAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCAtttt           >  7:268146/1‑139 (MQ=255)                                                                                                                              tCACGATAAATTTAACAATTGTTTTTTTATCTTAAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCTATTGC‑TCTTTTTAGAGCATTTTCACTTTAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGa   <  5:27869/138‑1 (MQ=255)                                                                                                                              tCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGa   <  1:108920/138‑1 (MQ=255)                                                                                                                              tCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGa   <  8:123190/138‑1 (MQ=255)                                                                                                                              tCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGa   <  6:158139/138‑1 (MQ=255)                                                                                                                              tCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGa   <  3:338523/138‑1 (MQ=255)                                                                                                                              tCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGAt  >  5:96100/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        AAGCTGGCGACGTATGCAGCGAGGTGTATTGAAAATGAGATTGTAATTACAAAAGGGGGGTGCATACACCCCTCTTTAATACGTTTCAATATATATGGTGTCAGAATCCACAATGGTAACTTCTTTCACGATAAAGTTAACAATTGTTTTTTTATCTTCAAGAGTTAAGTTATCTGCACCGATTGATTGAAAATAGTCGATGGC‑TCTTTTTAGAGCATTTTCACTTGAGCTCGTATCATCTAGGACTTTCATTTTTGACTGGAT  >  CP006881/2471150‑2471413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑