Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,751,749 A→G 7.0% V237V (GTA→GTG)  U712_14185 → Glycolate oxidase subunit glcD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,751,7490AG 7.0% 160.6 / 4.0 57V237V (GTA→GTG) U712_14185Glycolate oxidase subunit glcD
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base G (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

TCTTTTTGTCGGCTCAGAAGGAACACTCGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAGCGCAAAAGCAGTTCTGTTAATTGAA  >  CP006881/2751620‑2751878
                                                                                                                                 |                                                                                                                                 
tCTTTTTGTCGGCTCAGAAGGTACACTCGGCATTGTGACAGAGGCAATCGTGAACCTCGTTCGAACGCCGGAATCAAAGATGACGCTGCGTGCCCTGTATGAACACATCGATGCCGCTGCTCAAACGGTATCTGACatt                                                                                                                          >  2:179872/1‑139 (MQ=255)
          ggCTCATATGGAACACTCGGCATGGCTACAGAGGCAATCTTAAAACACGTTACAAAGCCGGAAACAACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCa                                                                                                                 <  7:306853/138‑1 (MQ=255)
                      acacTCGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCTCAAAAATTATACACg                                                                                                    >  6:125712/1‑139 (MQ=255)
                      acacTCGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCg                                                                                                    >  5:250335/1‑139 (MQ=255)
                           cGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACg                                                                                               >  1:164149/1‑139 (MQ=255)
                           cGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACg                                                                                               >  1:70700/1‑139 (MQ=255)
                           cGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACg                                                                                               >  4:227542/1‑139 (MQ=255)
                           cGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACg                                                                                               >  8:310859/1‑139 (MQ=255)
                               aTTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTGTCGGACATTATTGCCGCAAAAATTATACCCGCTACGCtt                                                                                            >  3:297764/1‑138 (MQ=255)
                               aTTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTAAAACGGTGTCGGACATTATTGCCGCAAAAATTATACCAGCTAAGcat                                                                                            >  7:175539/1‑136 (MQ=255)
                                  gtgACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAAt                                                                                        >  8:323005/1‑139 (MQ=255)
                                        gcggCAATCGTCAAACTCCTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAACCATCGATCCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTtctc                                                                                   >  3:21616/3‑138 (MQ=255)
                                        gagGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTtctc                                                                                   >  7:55488/1‑138 (MQ=255)
                                        gagGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTtctc                                                                                   >  7:50574/1‑138 (MQ=255)
                                        gagGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCGCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTtctc                                                                                   >  5:385326/1‑138 (MQ=255)
                                        gagGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTAAGAAAACATCGATGCCGCAGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTtctc                                                                                   >  7:65039/1‑138 (MQ=255)
                                         gggCAAGCGTAAAACTGCTTCAAAAGCCGGAACCAACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGa                                                                                 <  5:296655/138‑1 (MQ=255)
                                          ggCAATCGTCAAACTCTTACAAAAGCCGCAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  5:92269/139‑1 (MQ=255)
                                          ggCAATCGTCAAACTCGTTCCAAAGCCGGAAACAACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  2:8249/139‑1 (MQ=255)
                                          ggCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  7:323005/139‑1 (MQ=255)
                                          ggCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  5:140042/139‑1 (MQ=255)
                                          ggCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  1:38076/139‑1 (MQ=255)
                                          ggCAATCGGCAAACTCGTTCCAAAGCCGGAAACAAACAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  8:391556/139‑1 (MQ=255)
                                          gccAACCGTCAAACTCGTCCCAACGCCGGAAACAACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  8:65039/137‑1 (MQ=255)
                                           taaaTCGCAAAACTCGTCCCAAAGCGGGAAACCACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATc                                                                               <  6:23764/137‑1 (MQ=255)
                                           gCAATCGTAAAACTCGTTCCAACGCCGTAAACAAACAAGACGCTGCTTGCCCTTTATGAAAACCTCGATGCCGCTGCTCAAACTGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  2:127316/138‑1 (MQ=255)
                                               tCGCCAAACTCGTCCCAAACCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGcc                                                                           <  5:227375/139‑1 (MQ=255)
                                                 gTCAACCTCGTTCCAAAGCCGGAACCAAAGAAGACGCTGCTTGCCCTGTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  5:350391/139‑1 (MQ=255)
                                                 gTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTGTCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  4:297764/139‑1 (MQ=255)
                                                 gTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  6:389163/139‑1 (MQ=255)
                                                 gTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  8:50574/139‑1 (MQ=255)
                                                 gTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  8:55488/139‑1 (MQ=255)
                                                  taaaaCTCGTTCCAATGCAGTAACCAATGAAGACGCTGCTTGCCGTTTATGAAATCATCGATGCCGCTGCTCATACGGTGTCGGACATTTTTGCCGCAAATATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  8:175539/136‑1 (MQ=255)
                                                   cAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                >  5:356120/1‑130 (MQ=255)
                                                   cAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  6:356120/130‑1 (MQ=255)
                                                    aaaCTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTaca                                                                       <  7:40113/138‑1 (MQ=255)
                                                        acgaaccAAACCCGGAAACACAGAAGAAGCTTCTTGCCCTTTATGAAAACATCGATGCCGCTGCTGAATCGGTATCGGACATTCTTGACGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTAtt                                                                  <  5:125712/134‑1 (MQ=255)
                                                          gTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTgg                                                                >  4:244362/1‑139 (MQ=255)
                                                           ttCCAAAGCCGAAAACAACGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGt                                                               <  8:141422/139‑1 (MQ=255)
                                                               aaaGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGAtt                                                           <  7:310859/139‑1 (MQ=255)
                                                                      gAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTTCCGCAAAAATTATACACGCTACGATTGAATTTCTCGATCAGCCTACACTATAGGTGATTGAGGAt                                                     >  7:294515/1‑138 (MQ=255)
                                                                                    gctgctTGCCCTAACTGAAAACATCGAAGCCGCTGCTCAAACGGTATCGGACATTATTCCCGCAAAAAATATACCCGCTACGATTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGa                                      <  1:179872/139‑1 (MQ=255)
                                                                                         ttGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                >  2:312866/1‑92 (MQ=255)
                                                                                         ttGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  1:312866/92‑1 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  7:237712/85‑1 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                >  8:237712/1‑85 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         >  7:248338/1‑92 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTa                                                                         <  8:248338/92‑1 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACTCTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCTACAAgc                          >  6:263283/1‑139 (MQ=255)
                                                                                                ttATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAgc                          >  5:262794/1‑139 (MQ=255)
                                                                                                  aTGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAgcg                         >  3:207129/1‑138 (MQ=255)
                                                                                                       aaCATCGATGCCGCTTCTCAAACGGTATCGGACATTATTGAAGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTAGACTTTTGGTGATTGAGGATTATTCCAAAATCGGACTTCCGACAAGCGCAAAAg                   <  6:47453/139‑1 (MQ=255)
                                                                                                       aaCATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAGCGCAAAAg                   <  2:164149/139‑1 (MQ=255)
                                                                                                          aTCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCCACAAGCGCAAAAGCAg                >  5:47453/1‑139 (MQ=255)
                                                                                                                          aaaCGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTGCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAGCGCAAAAGCAGTTCTGTTAATTGaa  <  2:70700/137‑1 (MQ=255)
                                                                                                                            aCGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                <  2:367628/57‑1 (MQ=255)
                                                                                                                            aCGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGAt                                                                                >  1:367628/1‑57 (MQ=255)
                                                                                                                                 |                                                                                                                                 
TCTTTTTGTCGGCTCAGAAGGAACACTCGGCATTGTGACAGAGGCAATCGTCAAACTCGTTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATTATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAATCGGACTTCCGACAAGCGCAAAAGCAGTTCTGTTAATTGAA  >  CP006881/2751620‑2751878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑