Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,045,858 A→T 8.0% T142S (ACC→TCC)  U712_15645 → putative hydrolase yugF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,045,8580AT 8.0% 132.2 / 3.3 50T142S (ACC→TCC) U712_15645putative hydrolase yugF
Reads supporting (aligned to +/- strand):  ref base A (19/26);  new base T (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

TGCTTGTCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCAGA  >  CP006881/3045724‑3045986
                                                                                                                                      |                                                                                                                                
tgCTTGTCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCa                                                                                                                              >  7:57102/1‑139 (MQ=255)
  cTTGTCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCat                                                                                                                             <  7:222768/138‑1 (MQ=255)
  cTTGTCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCat                                                                                                                             <  7:346169/138‑1 (MQ=255)
      tCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATccc                                                                                                                        <  3:224512/139‑1 (MQ=255)
          gCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTAt                                                                                                                    >  8:139817/1‑139 (MQ=255)
           cATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCGCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTAtt                                                                                                                   <  7:125187/139‑1 (MQ=255)
             tgcTATGGGCTGGCATATATCGCTGTCTGCTGCGCTCCAAAAACCTGAGCTGTTTTCAAAGGTTGGGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTAtttt                                                                                                                 <  8:71896/137‑1 (MQ=255)
                tATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGTCCCCATATCCCGTATTTTCa                                                                                                               >  7:325722/1‑138 (MQ=255)
                        ggcAGATATCGCTGTTTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACAt                                                                                                      >  1:280018/1‑139 (MQ=255)
                        ggcAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACAt                                                                                                      >  7:241308/1‑139 (MQ=255)
                        ggcAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACAt                                                                                                      >  7:246934/1‑139 (MQ=255)
                        ggcAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACAt                                                                                                      >  3:169681/1‑139 (MQ=255)
                          cAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATc                                                                                                     >  1:112181/1‑138 (MQ=255)
                          cAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATc                                                                                                     >  4:334149/1‑138 (MQ=255)
                               atCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGc                                                                                               >  8:55053/1‑139 (MQ=255)
                                  gCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTgg                                                                                            <  5:121344/139‑1 (MQ=255)
                                  gCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTg                                                                                             <  8:241308/138‑1 (MQ=255)
                                  gCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTg                                                                                             <  8:246934/138‑1 (MQ=255)
                                      tCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGct                                                                                          <  5:321754/137‑1 (MQ=255)
                                       ctgctgCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCATTAAATAAAACGCTGGCactc                                                                                       >  5:59110/1‑139 (MQ=255)
                                       ctgctgCTCTCAAAAAACCTGTGCTTTTTTCAAAGGTTGTGCTGGTTTGCAGTTCAGGGTATTTAATACGTTCACACCGGACTTTTATTTTCGGGATTTATATCCCGTATTTTCATCTTTACATCAAACGCTGGctat                                                                                        <  8:358626/138‑3 (MQ=255)
                                        tgctgcTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGTCCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCtctc                                                                                       >  3:378787/1‑138 (MQ=255)
                                        tgctgcTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGTCCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCtctc                                                                                       >  3:373304/1‑138 (MQ=255)
                                             ctctCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCAGATTTACATCAACAGCTGGCTCTCGAaag                                                                                  >  6:321754/1‑138 (MQ=255)
                                                  cAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAACACGTTCACACCCGACGATTATTTTCGGGa                                                                                                                                  >  8:296588/1‑85 (MQ=255)
                                                  cAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGa                                                                                                                                  <  7:296588/85‑1 (MQ=255)
                                                  cAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCg                                                                            >  6:310538/1‑139 (MQ=255)
                                                            aGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGaaaaa                                                                  >  8:349617/1‑139 (MQ=255)
                                                                              tgctgcTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGGGATGAAAAATTTATTAAATGTGGTGCa                                                <  7:139817/139‑1 (MQ=255)
                                                                               gctgctTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATTAAAAATTTATTAAATGTGGTGCa                                                <  1:214098/138‑1 (MQ=255)
                                                                                   ctTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGTCCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACa                                           <  4:378787/139‑1 (MQ=255)
                                                                                   ctTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGTCCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACa                                           <  4:373304/139‑1 (MQ=255)
                                                                                          gTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCt                                    <  2:280018/139‑1 (MQ=255)
                                                                                          gTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCt                                    <  7:55053/139‑1 (MQ=255)
                                                                                             cAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGTGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCtga                                  <  8:325722/138‑1 (MQ=255)
                                                                                             cAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCtgat                                 >  2:234208/1‑139 (MQ=255)
                                                                                             cAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGAATGACAAATCGCtgat                                 >  3:27517/1‑139 (MQ=255)
                                                                                                ggTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTg                                                                                             >  1:374739/1‑76 (MQ=255)
                                                                                                ggTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTg                                                                                             <  2:374739/76‑1 (MQ=255)
                                                                                                     ttAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCtgattgat                             >  4:84067/1‑135 (MQ=255)
                                                                                                     ttAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCtgattgat                             <  3:84067/135‑1 (MQ=255)
                                                                                                        aaaCGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGgaga                      <  7:349617/139‑1 (MQ=255)
                                                                                                          aCGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAgatg                    >  7:129176/1‑139 (MQ=255)
                                                                                                             ttCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGAtt                 <  1:234208/139‑1 (MQ=255)
                                                                                                              tCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGCGATGAtt                 >  5:296471/1‑138 (MQ=255)
                                                                                                               cacaCCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGGTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTg                <  4:169681/138‑1 (MQ=255)
                                                                                                               cacaCCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTg                <  1:4494/138‑1 (MQ=255)
                                                                                                               cacaCCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTg                <  8:57102/138‑1 (MQ=255)
                                                                                                               acaccccGACTTTTATTTTCGGCACCAATATCGAGTTTTTTCATATTTACATAAAACGCTGGCTCTCTAAAGATGGCGTTATTAAAATTTTATTAAATGTGGTGCATTACAATTCGCTGATTGATGAGGAGATTATTg                <  6:59110/134‑1 (MQ=255)
                                                                                                                          attattTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGGGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCa    <  7:252715/139‑1 (MQ=255)
                                                                                                                             attTTCGTGACCCATATCCCGTATTTTCATCATTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGTTGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCAGa  <  4:120436/138‑1 (MQ=255)
                                                                                                                             attTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCAGa  <  3:334149/138‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                
TGCTTGTCGGGCATTCTATGGGCGGGCAGATATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTTCACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTGATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCAGA  >  CP006881/3045724‑3045986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑