Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	431,062	A→C	6.5%	G306G (GGT→GGG)	U712_01970 ←	putative aspartokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	431,062	0	A	C	6.5%	192.4 / 2.0	72	G306G (GGT→GGG)	U712_01970	putative aspartokinase
Reads supporting (aligned to +/- strand):  ref base A (39/28);  new base C (2/3);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAATGATTGTCATGTCATCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGCTCTGAATGCCGGAAT  >  CP006881/430924‑431193                                                                                                                                           |                                                                                                                                    tAAAATGATTGTCATGTCATCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCaaaaa                                                                                                                                     >  1:120971/1‑139 (MQ=255)      tGATTGTCATGTCATCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGgt                                                                                                                                <  5:187631/139‑1 (MQ=255)        aTTGTCATGTCATCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGgtgt                                                                                                                              >  8:180722/1‑139 (MQ=255)                 tcatCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCa                                                                                                                      <  7:198162/138‑1 (MQ=255)                 tcatCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCa                                                                                                                      <  6:306415/138‑1 (MQ=255)                      gATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTATGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGcc                                                                                                                 <  4:231405/138‑1 (MQ=255)                              aTGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAgg                                                                                                        <  7:180722/139‑1 (MQ=255)                                     aTGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGttt                                                                                                 >  8:256029/1‑139 (MQ=255)                                       gCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTtgt                                                                                               >  7:342284/1‑139 (MQ=255)                                        cTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAAGCGATTTCTCTGTTCATGAGATAATTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTtgtg                                                                                              >  7:250267/1‑139 (MQ=255)                                        cTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTtgtg                                                                                              <  7:256029/139‑1 (MQ=255)                                            tACGTCAAACCATGCTCCTCCAGGATTTGTAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTa                                                                                           >  7:397945/1‑138 (MQ=255)                                            tACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCgacgac                                                                                                           <  4:376968/122‑1 (MQ=255)                                            tACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCgacgac                                                                                                           >  3:376968/1‑122 (MQ=255)                                              cGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATcc                                                                                        <  2:120971/139‑1 (MQ=255)                                                 cAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGc                                                                                     >  5:43099/1‑139 (MQ=255)                                                   aaCCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCtt                                                                                   >  6:353774/1‑139 (MQ=255)                                                        tGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAACCCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTga                                                                              >  2:61661/1‑139 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCa                                                                                                                      >  2:169633/1‑98 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCa                                                                                                                      <  1:169633/98‑1 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAgg                                                                                                        >  3:149627/1‑112 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAgg                                                                                                        <  4:149627/112‑1 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGt                                                                                                   >  2:400953/1‑117 (MQ=255)                                                         gCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGt                                                                                                   <  1:400953/117‑1 (MQ=255)                                                          ctcctcCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTgacg                                                                            <  3:201649/139‑1 (MQ=255)                                                                cAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCgg                                                                      <  3:16309/139‑1 (MQ=255)                                                                cAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCgg                                                                      <  4:201920/139‑1 (MQ=255)                                                                       tGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCt                                                                                >  2:120588/1‑122 (MQ=255)                                                                       tGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCt                                                                                <  1:120588/122‑1 (MQ=255)                                                                       tGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGTTGCCtt                                                               >  5:293481/1‑139 (MQ=255)                                                                       tGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               >  5:259425/1‑139 (MQ=255)                                                                        gccgggCTCTGCGGCCAAAAGCGATTTCTCTGTTCATGAGATACTTGCTGATATAATTGCTGCAAACACCGGGGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               <  3:239629/135‑1 (MQ=255)                                                                              ctctGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               <  2:309680/132‑1 (MQ=255)                                                                              ctctGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               >  1:309680/1‑132 (MQ=255)                                                                              ctctGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCt                                                          >  7:26251/1‑137 (MQ=255)                                                                              ctctGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTCGCTGACGACGCGGGTGCCTTCGGCt                                                          >  4:80435/1‑137 (MQ=255)                                                                               tctGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGATGCAAAAACCGGTGTCGCTGGCAATGACGACGACAGGCCCGTTTGTGTTATCACGATTGCTGACGACGCGGGTGCCTTCGGCTGAg                                                       >  7:334511/1‑139 (MQ=255)                                                                                 tGCGGCGAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAACCCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGCCGACGCGGGTGCCATCGGCTGCggg                                                     >  2:81893/1‑139 (MQ=255)                                                                                 tGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAgg                                                                                                        <  3:355578/88‑1 (MQ=255)                                                                                 tGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAgg                                                                                                        >  4:355578/1‑88 (MQ=255)                                                                                 tGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAggg                                                     >  7:45018/1‑139 (MQ=255)                                                                                 tGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAggg                                                     >  1:215093/1‑139 (MQ=255)                                                                                      ccAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGgttgt                                                >  7:13981/1‑139 (MQ=255)                                                                                      ccAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGgtggg                                                >  8:299552/1‑136 (MQ=255)                                                                                        aaaaCCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTGCGGCTGAGGGGTTGTTc                                              >  1:340962/1‑139 (MQ=255)                                                                                         aaaCCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCg                                             <  2:222632/139‑1 (MQ=255)                                                                                               aTTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTAtttt                                       >  7:198760/1‑139 (MQ=255)                                                                                                tttCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAACCCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTAttttt                                      >  8:88286/1‑139 (MQ=255)                                                                                                tttCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGCCGCCAGGCCCGTTTGTGTTATCCCGCTTGATGACGACGCGGGTGACTTCGTCTGATGTGTTGTTCGTAgtttt                                      >  8:95148/1‑139 (MQ=255)                                                                                                tttCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTg                                                         <  2:250344/120‑1 (MQ=255)                                                                                                tttCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTg                                                         >  1:250344/1‑120 (MQ=255)                                                                                                   ctctGTTCATGAGATACTTGCTGATATAAATGCTGCAAAACCCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   <  7:88286/139‑1 (MQ=255)                                                                                                   cgctGTTCATGAGATACTTGCTGATCTAACTGCTGCAAAACCCGGTGTCGCTGGCAAGGCCGACGACAGGCCCGTTTGTGTTCTCCCGCTTGCTGCCGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   <  6:11519/137‑1 (MQ=255)                                                                                                      tGTTCATTAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   <  1:267207/136‑1 (MQ=255)                                                                                                      tGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAg                                                                                                         <  4:35983/66‑1 (MQ=255)                                                                                                      tGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAg                                                                                                         >  3:35983/1‑66 (MQ=255)                                                                                                      tGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   <  8:26251/136‑1 (MQ=255)                                                                                                      tGTTCATGAGATAATTGCTTATATAAATGCTGCAAAACCCGGTGTCTCTGTCAATGCCGACGACAGGCACGTTTGTGTTATCCCGCTGGCTGACGACGCGGGTGCCTTCGGATGAGGGGTTGTTCGTATTTTTGAt                                   <  7:95148/136‑1 (MQ=255)                                                                                                        ttCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGa                               <  5:353774/138‑1 (MQ=255)                                                                                                                  aCTTGCTGATATAAATGCTGCAAAAACCGGTGTCTCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               >  5:135877/1‑96 (MQ=255)                                                                                                                  aCTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCtt                                                               <  6:135877/96‑1 (MQ=255)                                                                                                                  aCTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATccc                    >  4:323067/1‑139 (MQ=255)                                                                                                                  aCTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATccc                    >  8:85895/1‑139 (MQ=255)                                                                                                                  aCTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCCGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATccc                    >  8:15418/1‑139 (MQ=255)                                                                                                                   cTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATcccc                   <  8:342284/139‑1 (MQ=255)                                                                                                                     tGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   >  2:126408/1‑121 (MQ=255)                                                                                                                     tGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGAt                                   <  1:126408/121‑1 (MQ=255)                                                                                                                     tGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTGGTTCGTATTTTTGATCTGAACAGGAATCCCCGc                 >  8:197785/1‑139 (MQ=255)                                                                                                                      gCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGct                >  1:278296/1‑139 (MQ=255)                                                                                                                        tGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGctct              <  8:397945/139‑1 (MQ=255)                                                                                                                          atatAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCAGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGCTCTg             >  8:400491/1‑138 (MQ=255)                                                                                                                                    tgcAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGCTCTGAATGCCGGAAt  >  3:120871/1‑139 (MQ=255)                                                                                                                                      cAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGCGCTGAATGCCGGAAt  >  1:283753/1‑137 (MQ=255)                                                                                                                                           |                                                                                                                                    TAAAATGATTGTCATGTCATCGATTCCCGATGGAACATGCTCATACGTCAAACCATGCTCCTCCAGGATTTGAAGGGCTCTGCGGCCAAAACCGATTTCTCTGTTCATGAGATACTTGCTGATATAAATGCTGCAAAAACCGGTGTCGCTGGCAATGCCGACGACAGGCCCGTTTGTGTTATCCCGCTTGCTGACGACGCGGGTGCCTTCGGCTGAGGGGTTGTTCGTATTTTTGATCTGAACAGGAATCCCCGCTCTGAATGCCGGAAT  >  CP006881/430924‑431193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑