BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,126,394	A→C	5.9%	I22S (ATT→AGT)	U712_16045 ←	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,126,394	0	A	C	5.9%	260.9 / 2.2	94	I22S (ATT→AGT)	U712_16045	Hypothetical Protein
Reads supporting (aligned to +/- strand): ref base A (56/32); new base C (2/4); total (58/36)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACAACGGATGATTCA  >  CP006881/3126259‑3126527

ttCTCAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCg                                                                                                                                    >  5:223483/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTTCCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCTGCCAAATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATctc                                                                                                                                   >  5:89031/1‑137 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGTTTGGGATCCTTGTCCAAGATCGc                                                                                                                                   >  7:137757/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCCAAATCGc                                                                                                                                   >  4:37813/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAACTCGc                                                                                                                                   >  8:386140/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  8:211903/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  3:386944/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  8:107616/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  5:73923/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  8:227971/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGc                                                                                                                                   >  1:395956/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACAGCAAGGTTGGGAGCCTTCTCCAAAATCGc                                                                                                                                   >  4:15749/1‑139 (MQ=255)

 tctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCAGATTCAAGGACATCAAGGTTGGGATCATTCTCCAAAATCGc                                                                                                                                   >  5:234334/1‑139 (MQ=255)

  ctcAACAAGCTCAGCAGGCGTTTCCCCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGcc                                                                                                                                  >  7:264501/1‑139 (MQ=255)

  ctcAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGcc                                                                                                                                  >  1:82739/1‑139 (MQ=255)

     aacaaGCTCAGCAGGCGTTTCTCCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATACTTATCCAACATCGCCCgg                                                                                                                               >  2:51509/1‑139 (MQ=255)

     aacaaGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCgg                                                                                                                               >  1:197108/1‑139 (MQ=255)

       cacgCTCCGCCGGCGTTTCACCCATCACGACTTCTCCGTTAACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAACCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGc                                                                                                                             <  2:82739/136‑1 (MQ=255)

       cacgCTCAGCCGGCGTTACACCCATGCCGACTTCTCCGTTTACCAGAGCTAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGc                                                                                                                             <  8:80050/136‑1 (MQ=255)

       caaGCTCCGCAGGCGTTTCACCCATGACGACTTCTCCGTTAACCAGAGCCAAACGCGATTCCATACATGGCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGc                                                                                                                             <  4:386944/139‑1 (MQ=255)

       caaCCTCCGCAGCGGTATCCCCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGc                                                                                                                             <  7:248942/139‑1 (MQ=255)

          gCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCCTACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGCCATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTc                                                                                                                          >  4:347242/1‑139 (MQ=255)

              agcagGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTACGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCt                                                                                                                        >  8:256213/1‑137 (MQ=255)

              agcagGCGTTTCACCCATCACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTga                                                                                                                      <  8:222089/139‑1 (MQ=255)

               gcagGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTgag                                                                                                                     >  6:73376/1‑139 (MQ=255)

                     gTTTCACCCATGACGAATTCTACGTTAACCAGCGCGAAAAGCTATTCATTACATGGCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATAAAGGTTGGTATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGt                                                                                                               <  8:264501/139‑1 (MQ=255)

                        tCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAg                                                                                                            >  7:211722/1‑139 (MQ=255)

                        tCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAg                                                                                                            >  7:397942/1‑139 (MQ=255)

                        tCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAg                                                                                                            >  5:280161/1‑139 (MQ=255)

                                     cTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGa                                                                                                >  4:190823/1‑138 (MQ=255)

                                     cTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCATAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGa                                                                                                >  2:332252/1‑138 (MQ=255)

                                        ctcCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGacac                                                                                             >  8:119420/1‑138 (MQ=255)

                                                aCCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCaa                                                                                    >  5:9860/1‑139 (MQ=255)

                                                     agCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCAtt                                                                               >  7:277619/1‑139 (MQ=255)

                                                     agCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCGGACACAAAATTCAATCAtt                                                                               >  8:99910/1‑139 (MQ=255)

                                                            aGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAACTCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCa                                                                        >  5:49290/1‑139 (MQ=255)

                                                              cGATTCCATACATGTCCCGCAGTAGCTTACGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATg                                                                      <  7:256213/139‑1 (MQ=255)

                                                               gATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGCGAGCCGTGAGCCAGATTGCTGCCACAAAATTCAAGCATTGGATTCATGc                                                                     >  5:119085/1‑139 (MQ=255)

                                                                    cATACATTCCCCGCCGTAGCTTAAGCAGCCATATTCACGCACATCAAGGTTGGTATCCTTCTCCAAACTCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGttt                                                                <  3:37813/139‑1 (MQ=255)

                                                                    cATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGttt                                                                <  7:96808/139‑1 (MQ=255)

                                                                    cATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGtt                                                                 <  7:119420/138‑1 (MQ=255)

                                                                    cATACATGGCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGGTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGttt                                                                <  1:51509/139‑1 (MQ=255)

                                                                      tACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCa                                                              <  1:332252/139‑1 (MQ=255)

                                                                      tACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCa                                                              >  2:215794/1‑139 (MQ=255)

                                                                        cATGTCGCGAAGTAGCTTAAGCTGCAATATGCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTGCTGAGAGCCGTGAGCCAGATTGCTGACACAAAAATCAATCATTGGATTCATGCGGTTTCAc                                                             <  4:58398/138‑1 (MQ=255)

                                                                        cATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCAcc                                                            <  6:280161/139‑1 (MQ=255)

                                                                        cATGTCCCGCAGTAGATTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCAc                                                             <  8:211722/138‑1 (MQ=255)

                                                                         aTGTCCCGCAGTAGCTTAAGCAGCCATATTCAATGACATCAAGGTTGGGATCGTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCt                                                           <  7:227971/139‑1 (MQ=255)

                                                                         aTGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAACTCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCt                                                           <  7:386140/139‑1 (MQ=255)

                                                                         aTGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCt                                                           <  1:215794/139‑1 (MQ=255)

                                                                         aTGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCt                                                           <  6:73923/139‑1 (MQ=255)

                                                                           gTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTca                                                         >  3:316459/1‑139 (MQ=255)

                                                                           gTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTca                                                         >  8:239350/1‑139 (MQ=255)

                                                                           gTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTAGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATTCGGTTTCAGCTca                                                         >  3:58398/1‑139 (MQ=255)

                                                                              ccgccgTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACat                                                      <  6:89031/139‑1 (MQ=255)

                                                                              ccGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTcaca                                                       >  8:18421/1‑138 (MQ=255)

                                                                              ccGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACat                                                      <  3:347242/139‑1 (MQ=255)

                                                                                gacgTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGCTCCTTCTCCAAACTCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACatat                                                    <  3:15749/136‑1 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGAcaca                                                                                            >  3:387021/1‑83 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGAcaca                                                                                            <  4:387021/83‑1 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     >  1:1791/1‑90 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     <  2:1791/90‑1 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAAt                                    >  1:194939/1‑139 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAAt                                    >  7:310268/1‑139 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAAt                                    >  4:3304/1‑139 (MQ=255)

                                                                                                ccATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATAGCGAAt                                    >  5:299380/1‑139 (MQ=255)

                                                                                                     ttCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAAttttt                                <  7:18421/138‑1 (MQ=255)

                                                                                                          ggACATCACGGGTGGGAGCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTAt                          <  8:220110/139‑1 (MQ=255)

                                                                                                          ggACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCa                                                                        >  7:183735/1‑93 (MQ=255)

                                                                                                          ggACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCa                                                                        <  8:183735/93‑1 (MQ=255)

                                                                                                          ggACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTAt                          <  7:211903/139‑1 (MQ=255)

                                                                                                          ggACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTAt                          >  2:139026/1‑139 (MQ=255)

                                                                                                          ggACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTAACTAt                          >  8:59263/1‑139 (MQ=255)

                                                                                                            aCATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTgag                                                                                                                     <  4:99086/46‑1 (MQ=255)

                                                                                                            aCATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTgag                                                                                                                     >  3:99086/1‑46 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     >  3:115003/1‑76 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     <  4:115003/76‑1 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     <  4:109304/76‑1 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCa                                                                                     >  3:109304/1‑76 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACat                                                      >  1:44049/1‑107 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACat                                                      <  2:44049/107‑1 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGt                      >  3:73267/1‑139 (MQ=255)

                                                                                                              aTCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATACAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGt                      >  5:166349/1‑139 (MQ=255)

                                                                                                               tCACGGTGGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTc                     <  6:223483/139‑1 (MQ=255)

                                                                                                               tCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGCCACAAAATTCAATCATTGGATTCATGCGGTTTGACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGt                      >  6:119201/1‑138 (MQ=255)

                                                                                                               tCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATg                                                                      >  7:86265/1‑90 (MQ=255)

                                                                                                               tCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATg                                                                      <  8:86265/90‑1 (MQ=255)

                                                                                                               tCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAAACATTTGATTCATGCGGTTTCACCTCACATATGTACATTATCGCGAAGTTTTAACTATTCGt                      >  2:284374/1‑138 (MQ=255)

                                                                                                                       gggATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACaa             >  1:91826/1‑139 (MQ=255)

                                                                                                                        ggATCCTTCTCCAAACTCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTAt                          <  7:331142/125‑1 (MQ=255)

                                                                                                                        ggATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTAt                          >  8:331142/1‑125 (MQ=255)

                                                                                                                              ttCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACAACGgatga      >  8:3699/1‑139 (MQ=255)

                                                                                                                               tctcCAAAATCGCCCGGGCTTCCTGAGCGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCAGATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACAACGgatgat     >  3:93481/1‑139 (MQ=255)

                                                                                                                                   cAACATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACAACGGATGATTCa  <  8:397942/138‑1 (MQ=255)

TTCTCAACAAGCTCAGCAGGCGTTTCACCCATGACGACTTCTCCGTTTACCAGAGCGAAAAGCGATTCCATACATGTCCCGCAGTAGCTTAAGCAGCCATATTCAAGGACATCAAGGTTGGGATCCTTCTCCAAAATCGCCCGGGCTTCCTGAGAGCCGTGAGCCAGATTGCTGACACAAAATTCAATCATTGGATTCATGCGGTTTCACCTCACATATGTCCATTATCGCGAATTTTTCACTATTCGTCAAAGACAACGGATGATTCA  >  CP006881/3126259‑3126527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`