Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,049	T→C	K40K (AAA→AAG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,049	0	T	C	100.0%	119.9 / NA	33	K40K (AAA→AAG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/29);  total (4/29)

TGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724917‑3725183                                                                                                                                     |                                                                                                                                        tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                                    <  4:44082/138‑1 (MQ=255)  ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  3:32498/139‑1 (MQ=255)   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  8:215654/138‑1 (MQ=255)   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  7:33067/138‑1 (MQ=255)   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  7:33448/138‑1 (MQ=255)   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  2:326682/138‑1 (MQ=255)   gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                                  <  3:151781/138‑1 (MQ=255)             gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                                        <  6:150215/138‑1 (MQ=255)             gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                                        <  6:157094/138‑1 (MQ=255)              aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  3:331609/139‑1 (MQ=255)              aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  2:262593/139‑1 (MQ=255)              aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                      <  5:109700/139‑1 (MQ=255)                 gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                                   <  8:429642/139‑1 (MQ=255)                      cAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                              <  6:36315/139‑1 (MQ=255)                      cAAAAATCTTCTTTTTTAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATACCCAGCCACCCGCCATACATACACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGaa                                                                                                              <  6:286639/139‑1 (MQ=255)                                     ataacccgcgGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCCCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  2:263040/132‑1 (MQ=255)                                     ataAAGCGGGGCGGCTTTCGCTTTCGGCTTTTCTACCTCATTCACAGCCATCCGCCATTAATAAAACGAAACGAGGCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  2:116909/138‑1 (MQ=255)                                     ataAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                                <  1:146273/138‑1 (MQ=255)                                      taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                              <  8:86275/139‑1 (MQ=255)                                         aGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑g                                                                                          <  3:322458/139‑1 (MQ=255)                                                   ctttcgctttcgGCTTTTCTTCCTCATTCACCCCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  3:283281/139‑1 (MQ=255)                                                   cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  4:252576/139‑1 (MQ=255)                                                   cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTc                                                                                <  6:103461/139‑1 (MQ=255)                                                                        ccTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACCGCCGCCTTTCTTTGGTATTTACGCGGATCCCAAATGACCGCGGCACCCGCATGTTTTTAT‑TACGGCGTACACCACTtcatca                                                            >  7:370876/1‑138 (MQ=255)                                                                             ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                      <  3:147640/139‑1 (MQ=255)                                                                                    ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  4:426809/139‑1 (MQ=255)                                                                                    ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                               <  8:171393/139‑1 (MQ=255)                                                                                      aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                                             <  5:229988/139‑1 (MQ=255)                                                                                            cattCATACAACGACACGCGTCCTGCGGCCTGCAGCGCCGACTTTCTTTGGTATGTACGCTGAGCCTAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGtt                                       <  7:327411/136‑2 (MQ=255)                                                                                            cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCTTCCACACCCGTa                                       <  6:178803/136‑1 (MQ=255)                                                                                            cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                       <  1:322895/136‑1 (MQ=255)                                                                                                       cGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                            <  1:93790/139‑1 (MQ=255)                                                                                                         aaaCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCaa                          <  4:176488/139‑1 (MQ=255)                                                                                                          aaCGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCGGAGCCGAATAGACCGCGGCCGCCGCA‑GGGTTTCTGTGCGGCGTCACCCAGTTCATCAAAAAGCCCAGCCCCACCCCGAAAGATACACGCaaa                         >  3:390946/1‑139 (MQ=255)                                                                                                               gTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt                    <  5:373851/139‑1 (MQ=255)                                                                                                                      ggCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa             <  5:187599/139‑1 (MQ=255)                                                                                                                      ggCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa             <  3:12298/139‑1 (MQ=255)                                                                                                                          tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCCGCCGCA‑GTTTTTGTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGGAAACATACAAGCAAACGGTTCAAAGaaaaa          >  4:342263/1‑138 (MQ=255)                                                                                                                                 ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTc  >  1:255141/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        TGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCA‑GTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTC  >  CP006881/3724917‑3725183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑