Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	683,800	A→G	Q466R (CAG→CGG)	U712_03345 →	ATP‑dependent DNA helicase pcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	683,800	0	A	G	100.0%	266.7 / NA	70	Q466R (CAG→CGG)	U712_03345	ATP‑dependent DNA helicase pcrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/53);  total (17/53)

GAGTCGGCGCGACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACAGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAATCGATCGAAGCCCAAAGCCGTTTAGAAAA  >  CP006881/683665‑683935                                                                                                                                        |                                                                                                                                        gAGTCGGCGCGACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGgat                                                                                                                                      <  4:287151/139‑1 (MQ=255) gAGTCGGCGCGACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGgat                                                                                                                                      <  5:39479/139‑1 (MQ=255)      ggCGAGAAATCACTTGAAAAAATCGCTTCTTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGGCAGTGACAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTg                                                                                                                                 <  6:382400/139‑1 (MQ=255)       gcgcGACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTg                                                                                                                                 >  5:230116/1‑138 (MQ=255)        cgcgACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGaa                                                                                                                               <  1:52753/139‑1 (MQ=255)            aCATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGgat                                                                                                                                      >  7:118569/1‑128 (MQ=255)            aCATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGgat                                                                                                                                      <  8:118569/128‑1 (MQ=255)            aCATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAAcc                                                                                                                           >  2:116940/1‑139 (MQ=255)            aCATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAAc                                                                                                                            <  5:72551/138‑1 (MQ=255)            aCATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGACCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAAc                                                                                                                            >  6:72551/1‑138 (MQ=255)             cATCACTTGAAAAAATCGCTTCGTATGCGTCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCt                                                                                                                          <  2:35769/139‑1 (MQ=255)             cATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCt                                                                                                                          <  6:129405/139‑1 (MQ=255)                 aCTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGAc                                                                                                                       <  2:254564/138‑1 (MQ=255)                 aCTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGAc                                                                                                                       <  6:21272/138‑1 (MQ=255)                  cTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCa                                                                                                                     <  8:433061/139‑1 (MQ=255)                      aaaaaaTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAata                                                                                                                  <  7:203607/138‑1 (MQ=255)                       aacaaTCGCTTCGTATGCGGCCATAATCGGCTTGTCCTTTATCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATg                                                                                                                <  5:433816/136‑1 (MQ=255)                       aaaaaTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATg                                                                                                                <  1:99331/139‑1 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATg                                                                                                                <  1:339931/135‑1 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATg                                                                                                                >  2:339931/1‑135 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAgg                                                                                                            <  5:312281/139‑1 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAgg                                                                                                            <  6:383561/139‑1 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAgg                                                                                                            <  2:259413/139‑1 (MQ=255)                           aTCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAgg                                                                                                            <  1:113114/139‑1 (MQ=255)                                  cGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTAc                                                                                                       <  7:239430/137‑1 (MQ=255)                                  cGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTAc                                                                                                       <  2:22308/137‑1 (MQ=255)                                  cGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTAc                                                                                                       <  1:42198/137‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAATCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  5:234988/138‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  7:259036/138‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  8:152017/138‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  5:32175/138‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  5:423012/138‑1 (MQ=255)                                   gTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACtt                                                                                                     <  3:335493/138‑1 (MQ=255)                                      tGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATc                                                                                                  <  7:255439/138‑1 (MQ=255)                                      tGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATc                                                                                                  <  7:369718/138‑1 (MQ=255)                                      tGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATc                                                                                                  <  3:366229/138‑1 (MQ=255)                                          gCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTa                                                                                             <  6:32858/139‑1 (MQ=255)                                              tAAACGGCTTGTCCTTTTTCCAAGAGATTCAGCAGGTATATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCTCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACATATCCATTACa                                                                                           >  4:417790/1‑137 (MQ=255)                                                     cTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCt                                                                                                                          <  5:201379/99‑1 (MQ=255)                                                     cTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCt                                                                                                                          >  6:201379/1‑99 (MQ=255)                                                     cTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTAc                                                                                                       >  5:81871/1‑118 (MQ=255)                                                     cTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTAc                                                                                                       <  6:81871/118‑1 (MQ=255)                                                     cTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGa                                                                                    >  5:84203/1‑137 (MQ=255)                                                       tGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGAAGGATTACTTATCCATTACAGAGCTGACag                                                                                 >  4:272337/1‑138 (MQ=255)                                                              tttCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAAt                                                                          >  6:227339/1‑138 (MQ=255)                                                               ttCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAAtt                                                                         <  7:39933/138‑1 (MQ=255)                                                                 ccAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCt                                                                       <  8:273650/138‑1 (MQ=255)                                                                 ccAAGCGATTCAGCAGGTTGATTTTATCGGCGGCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATAACTTATCCATTACCGAGCTGACAGAAGAAATTCt                                                                       <  2:105334/138‑1 (MQ=255)                                                                     tcGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGAGGCAAACGCTCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTtata                                                                  <  3:183451/138‑1 (MQ=255)                                                                     gCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATa                                                                  <  6:230116/139‑1 (MQ=255)                                                                      cGAATCAGCAGGTTGTTTTTATCGGCGTCAGTGCCAAAGCGGCATACGCGCTTGACAGCTTTAGATGGATGATTGAAAAGCTGACCAATATGCAGGATTACTTATCCATTACAGCGCTGTCAGAAGAAATTCTTGATa                                                                  <  8:354727/138‑1 (MQ=255)                                                                       gATTCAGCAGGTTGATTTTATCGGCGGCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAg                                                                <  6:15068/139‑1 (MQ=255)                                                                        aTTCTGCAGGTTTATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGa                                                               <  2:83154/139‑1 (MQ=255)                                                                           cagcagGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACg                                                             <  7:75050/138‑1 (MQ=255)                                                                           cagcagGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACg                                                             <  7:47952/138‑1 (MQ=255)                                                                           cagcagGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACg                                                             <  6:84203/138‑1 (MQ=255)                                                                           cagcagGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACg                                                             <  3:68979/138‑1 (MQ=255)                                                                           cagcagGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCCGACAGAAGAAATTCTTGATAAGACg                                                             <  4:40767/138‑1 (MQ=255)                                                                                   tGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACa                                                      >  5:305376/1‑137 (MQ=255)                                                                                             ggCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTg                                           <  8:423755/138‑1 (MQ=255)                                                                                                gTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAgg                                       >  2:102736/1‑139 (MQ=255)                                                                                                     tGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCt                                     >  7:32593/1‑136 (MQ=255)                                                                                                         aaaGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAat                              <  1:102736/139‑1 (MQ=255)                                                                                                         aaaGCGGCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAat                              <  8:420842/139‑1 (MQ=255)                                                                                                               gCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAatcgatcg                        <  7:162478/139‑1 (MQ=255)                                                                                                               gCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAatcgatc                         >  1:108282/1‑138 (MQ=255)                                                                                                               gCAAACGCGCTTGACAGCTTTAGACGGATGATTGAAAAACTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAatcgatcg                        <  4:137228/139‑1 (MQ=255)                                                                                                                                   tAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATa                                                        >  3:258689/1‑87 (MQ=255)                                                                                                                                   tAGACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATa                                                        <  4:258689/87‑1 (MQ=255)                                                                                                                                     gACGGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAATCGATCGAAGCCCAAAGCCGTTCAGaaaa  >  3:148730/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GAGTCGGCGCGACATCACTTGAAAAAATCGCTTCGTATGCGGCCATAAACGGCTTGTCCTTTTTCCAAGCGATTCAGCAGGTTGATTTTATCGGCGTCAGTGCCAAAGCGGCAAACGCGCTTGACAGCTTTAGACAGATGATTGAAAACCTGACCAATATGCAGGATTACTTATCCATTACAGAGCTGACAGAAGAAATTCTTGATAAGACGGAATACAGAGAAATGCTGAAGGCTGAGAAATCGATCGAAGCCCAAAGCCGTTTAGAAAA  >  CP006881/683665‑683935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑