Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,092	T→C	A245A (GCA→GCG)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,092	0	T	C	100.0%	348.8 / NA	94	A245A (GCA→GCG)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/52);  total (42/52)

AACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGAC  >  CP006881/3723961‑3724220                                                                                                                                    |                                                                                                                                 aaCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCt                                                                                                                           <  6:89217/139‑1 (MQ=255)      ccAGAGATCACTCCCTGCAAGGCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAg                                                                                                                       <  2:54978/138‑1 (MQ=255)        agagATCACTGCCTGCAAGTCAAAGCGGTTTTTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGTTTCCGGCAAATGTAATTGATTTTCATTTTTCAAATACTCCGCCAGCTCCAgcgc                                                                                                                    <  2:366467/139‑1 (MQ=255)        agagATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgcgc                                                                                                                    <  6:358271/139‑1 (MQ=255)        acagctCACACCCTGCACCGCAACGCGGCTATTCCGAGAGATCTTGAAGACGCCGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCCGCTCCAgcgc                                                                                                                    <  6:304749/134‑1 (MQ=255)         gagaTCACTCCCTGCAAGTCTAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCTca                                                                                                                   <  1:420788/139‑1 (MQ=255)         gagaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGca                                                                                                                   <  5:273323/139‑1 (MQ=255)            aTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCCGCGCACAt                                                                                                                >  5:309647/1‑139 (MQ=255)               actgacTGCAAGTCAAAGCGTTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGa                                                                                                             <  7:175900/134‑1 (MQ=255)               aCTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGa                                                                                                             <  5:72366/139‑1 (MQ=255)                     tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc                                                                                                        >  2:121919/1‑138 (MQ=255)                      gCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcg                                                                                                       >  7:89592/1‑137 (MQ=255)                           tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa                                                                                                 >  6:186879/1‑139 (MQ=255)                           tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa                                                                                                 >  2:32321/1‑139 (MQ=255)                           tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa                                                                                                 <  4:123734/139‑1 (MQ=255)                               aGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACt                                                                                             >  7:256836/1‑139 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGcc                                                                                                                               <  7:334303/100‑1 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGcc                                                                                                                               >  8:334303/1‑100 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                        >  3:360545/1‑139 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                        >  1:21643/1‑139 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                        >  3:354064/1‑139 (MQ=255)                                    ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCCCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                        >  2:393944/1‑139 (MQ=255)                                         ccGAGAGATCTTGAAGATGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  2:414929/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  4:99044/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  5:119706/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  7:291148/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  7:361776/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  1:302917/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  8:333567/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  8:359610/138‑1 (MQ=255)                                         ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGAt                                                                                   >  7:110815/1‑139 (MQ=255)                                          cGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGAt                                                                                   <  3:353704/138‑1 (MQ=255)                                            agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  2:304775/135‑1 (MQ=255)                                            agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    >  1:304775/1‑135 (MQ=255)                                                aTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                             >  5:29173/1‑138 (MQ=255)                                                 tCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                             >  7:120751/1‑137 (MQ=255)                                                  cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    <  3:197226/129‑1 (MQ=255)                                                  cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                    >  4:197226/1‑129 (MQ=255)                                                  cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAg                                                                          >  4:132793/1‑139 (MQ=255)                                                     gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         <  8:63521/137‑1 (MQ=255)                                                     gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         <  3:94182/137‑1 (MQ=255)                                                     gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         <  3:43805/137‑1 (MQ=255)                                                        gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                    <  8:256836/139‑1 (MQ=255)                                                        gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                    <  5:274501/139‑1 (MQ=255)                                                           gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                 >  2:390430/1‑139 (MQ=255)                                                           gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                 >  7:20074/1‑139 (MQ=255)                                                           gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                 <  2:128772/139‑1 (MQ=255)                                                             tGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                               >  2:301287/1‑139 (MQ=255)                                                               gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                               <  8:416550/137‑1 (MQ=255)                                                               gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                               <  1:301287/137‑1 (MQ=255)                                                                aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa                                                            <  7:242202/139‑1 (MQ=255)                                                                aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa                                                            <  2:129716/139‑1 (MQ=255)                                                                aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTAATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa                                                            <  1:311706/139‑1 (MQ=255)                                                                    gCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATAc                                                         >  7:104254/1‑138 (MQ=255)                                                                       gCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATg                                                     >  5:85676/1‑139 (MQ=255)                                                                         gTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATg                                                     >  6:212978/1‑137 (MQ=255)                                                                          tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATTTTCATTTTTCAAAACCTACGCCATCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                  <  4:184158/139‑1 (MQ=255)                                                                             tataGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAgcgc                                               <  4:374003/139‑1 (MQ=255)                                                                             tataGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAgcg                                                <  7:190177/138‑1 (MQ=255)                                                                               taGCCTGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCt                                              <  2:239409/138‑1 (MQ=255)                                                                                aGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCGGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                             >  2:103294/1‑106 (MQ=255)                                                                                aGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCGGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                             <  1:103294/106‑1 (MQ=255)                                                                                  ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCTAATGAATACTTTTTTTGGATGAAATAAAGAATCTCCTCGTCCATACGATGGAGCGCTTCAt                                          >  5:316282/1‑139 (MQ=255)                                                                                  ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAATCAACTCCTCGTACATACGATGGAGCTCTTCAt                                          >  4:225938/1‑139 (MQ=255)                                                                                  ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAt                                          >  2:157833/1‑139 (MQ=255)                                                                                  ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAt                                          >  7:416921/1‑139 (MQ=255)                                                                                  ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAt                                          >  1:352595/1‑139 (MQ=255)                                                                                    ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAttt                                        >  8:228182/1‑139 (MQ=255)                                                                                    ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAACTCCTCGTCCATACGATGGAGCGCTTCAttt                                        >  8:287136/1‑139 (MQ=255)                                                                                     gCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAACCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTg                                       >  3:401749/1‑139 (MQ=255)                                                                                     gCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATTAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTGCATTTg                                       >  8:168686/1‑139 (MQ=255)                                                                                      ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGaaa                                                                               <  4:252480/98‑1 (MQ=255)                                                                                      ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGaaa                                                                               >  3:252480/1‑98 (MQ=255)                                                                                      ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         >  6:406830/1‑104 (MQ=255)                                                                                      ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         <  5:406830/104‑1 (MQ=255)                                                                                      ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGa                                      <  2:168797/139‑1 (MQ=255)                                                                                      acTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCTTCCATACGATGGAGAGATTCATTTGa                                      >  2:42073/2‑139 (MQ=255)                                                                                        tCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGAtt                                    >  4:409790/1‑139 (MQ=255)                                                                                          ggATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAGTGATAGGCGAATGAATACTTTTTTTGGAGGAAATTTAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGAtt                                    <  7:273541/137‑1 (MQ=255)                                                                                                      aTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                 <  6:186232/96‑1 (MQ=255)                                                                                                      aTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                 >  5:186232/1‑96 (MQ=255)                                                                                                      aTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgacc                      >  7:348366/1‑138 (MQ=255)                                                                                                       tGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATCGGCGAATGAATACTTTTTTTGGCTGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgaccg                     <  5:186879/139‑3 (MQ=255)                                                                                                       tGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgaccg                     <  1:32321/139‑3 (MQ=255)                                                                                                         tAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACCTTGATAGGCGAATGAATACTTTTCTTGGATGAAATAAAGCATCTCCTCCTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACcgcg                   <  8:289084/139‑1 (MQ=255)                                                                                                         tAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACcgcg                   <  8:20074/139‑1 (MQ=255)                                                                                                          aaTTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACcgcg                   <  1:121919/138‑1 (MQ=255)                                                                                                                 atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCTCTTCATTTGATTCCAACCGGCTGACAGCGTATCGCa            >  8:117686/1‑138 (MQ=255)                                                                                                                 atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa            >  8:284786/1‑138 (MQ=255)                                                                                                                     atttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATg        <  3:116087/136‑1 (MQ=255)                                                                                                                         ttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         >  3:159308/1‑69 (MQ=255)                                                                                                                         ttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                         <  4:159308/69‑1 (MQ=255)                                                                                                                           cAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc  <  1:393944/138‑1 (MQ=255)                                                                                                                           cAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc  <  1:123710/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 AACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGAC  >  CP006881/3723961‑3724220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑