Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,579,785 Δ1 bp 100% coding (73/132 nt) U712_18380 → Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,579,7850A.97.3% 312.3 / 1.2 73coding (73/132 nt)U712_18380Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (36/35);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCC  >  CP006881/3579647‑3579913
                                                                                                                                           |                                                                                                                                
cTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCtgcatgc‑a                                                                                                                                  <  5:284486/139‑1 (MQ=255)
 tGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAATCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCtgcatgc‑a                                                                                                                                  >  4:125807/1‑138 (MQ=255)
 tGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCtgcatgc‑a                                                                                                                                  >  6:198790/1‑138 (MQ=255)
     gcaagcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             <  1:96453/139‑1 (MQ=255)
       cagcaTACAATAGACATAGCTAGTAGATGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaaca                                                                                                                           <  1:196942/138‑3 (MQ=255)
        agcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaaca                                                                                                                           >  3:1024/1‑136 (MQ=255)
         gcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTAGTTCGTGTGTCTGCATGC‑aaacaacaca                                                                                                                         >  2:207519/1‑135 (MQ=255)
                 tAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGATATGAATATCAAAAAAACGGATGAAGTCGGATTCAATCCGAAACCGCACTTTTTATTTCGGCTGTCTGCATGC‑‑AAAAAACACACCCGCATc                                                                                                                >  2:196827/1‑139 (MQ=255)
                 tAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGa                                                                                                                  <  6:205219/137‑1 (MQ=255)
                 tAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGa                                                                                                                  >  5:205219/1‑137 (MQ=255)
                 tAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATc                                                                                                                >  8:17302/1‑139 (MQ=255)
                    aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTc                                                                                                             >  4:192108/1‑139 (MQ=255)
                    aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTc                                                                                                             >  2:364174/1‑139 (MQ=255)
                    aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTc                                                                                                             >  5:298329/1‑139 (MQ=255)
                    aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCAGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGTAATCAGTCCGAAACCGCACTTTTTATTTCGGTTGTCTGCATG‑‑AAAAAAACACACCCGGATCGTc                                                                                                             >  4:22687/1‑139 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             <  1:93162/122‑1 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             <  7:196317/122‑1 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             <  1:303335/122‑1 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             >  2:303335/1‑122 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             >  2:93162/1‑122 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑aaaaaa                                                                                                                             >  8:196317/1‑122 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTg                                                                                                           >  2:100496/1‑139 (MQ=255)
                      aTCTCTAGTAGAGGAAATCAACAAAGCAAAGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATACGAAACAGAACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACACGGATCGTCTg                                                                                                           >  1:96341/1‑139 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGACACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTgta                                                                                                         >  8:374627/1‑136 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTggt                                                                                                         >  1:310032/1‑137 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  5:373392/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  1:127733/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  5:70987/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  7:157534/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  1:66507/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         >  1:37228/1‑138 (MQ=255)
                         tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATCAAGTCTGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGAGCGTCTGGa                                                                                                         >  6:376667/1‑138 (MQ=255)
                           tagtatAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGTTGAAGTCGGATTCACTCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGa                                                                                                         <  1:342092/136‑1 (MQ=255)
                           tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTgg                                                                                                          <  8:137024/135‑1 (MQ=255)
                             gtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGaaaa                                                                                                      <  6:76687/137‑1 (MQ=255)
                                 agGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                >  5:282904/1‑139 (MQ=255)
                                  ggAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                >  8:362866/1‑138 (MQ=255)
                                   gtaaTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATACCAAACCGCACTGTTTATTTATGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCCTCAGGAAAAGATcaac                                                                                               >  8:46722/3‑138 (MQ=255)
                                   gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                               >  1:107981/1‑138 (MQ=255)
                                   gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                               >  7:373315/1‑138 (MQ=255)
                                   gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                               >  3:151115/1‑138 (MQ=255)
                                   gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGGCTGGAAAATCTcaac                                                                                               >  2:398603/1‑138 (MQ=255)
                                   gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATAAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACAATTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                               >  5:293434/1‑138 (MQ=255)
                                     aaTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAAt                                                                                            >  3:77046/1‑139 (MQ=255)
                                       tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                          >  2:13299/1‑139 (MQ=255)
                                       tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                          <  1:45905/139‑1 (MQ=255)
                                       tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                          <  1:241201/139‑1 (MQ=255)
                                          acaaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCggg                                                                                       <  6:70987/139‑1 (MQ=255)
                                            aaaGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTg                                                                                     <  8:373315/139‑1 (MQ=255)
                                                   cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                              >  5:212579/1‑139 (MQ=255)
                                                   cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                              >  5:378153/1‑139 (MQ=255)
                                                        ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATcttctt                                                                          >  4:98279/1‑138 (MQ=255)
                                                        ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATcttctt                                                                          >  2:363346/1‑138 (MQ=255)
                                                                   tGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttt                                                              <  2:304055/139‑1 (MQ=255)
                                                                     aaTATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtttttt                                                             <  6:378153/138‑1 (MQ=255)
                                                                         tCAAAAAAACGGATGAAGTCGGACTCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                        <  5:141922/139‑1 (MQ=255)
                                                                         tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                        <  1:290068/139‑1 (MQ=255)
                                                                         tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                        <  5:126862/139‑1 (MQ=255)
                                                                         tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                        <  5:56490/139‑1 (MQ=255)
                                                                         tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAGCTTCTTTAATACATTTTTTTGCgt                                                        <  7:46722/139‑1 (MQ=255)
                                                                                             ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                    <  6:298329/139‑1 (MQ=255)
                                                                                             ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                    <  6:282904/139‑1 (MQ=255)
                                                                                                  caatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                               >  6:345511/1‑139 (MQ=255)
                                                                                                   aatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                               <  6:388467/138‑1 (MQ=255)
                                                                                                    atcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCGTCAGTCAAAAATTcaac                              >  8:230879/1‑138 (MQ=255)
                                                                                                     tcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtc                                                                               >  1:150079/1‑88 (MQ=255)
                                                                                                     tcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtc                                                                               <  2:150079/88‑1 (MQ=255)
                                                                                                             cGCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGc                    <  1:364174/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                   <  4:403204/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                   <  4:77046/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                   <  5:198790/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                   <  5:345511/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                   <  5:74280/139‑1 (MQ=255)
                                                                                                              gCACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGGCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGGCAAAAATTCAACAAAACTCCGCa                   <  3:125807/139‑1 (MQ=255)
                                                                                                               cACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                  <  7:362866/139‑1 (MQ=255)
                                                                                                               cACTTTTTATTTCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                  >  6:139824/1‑139 (MQ=255)
                                                                                                                     ttATTTCGGGTGGCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTGCGCATCGCaa             <  8:157534/138‑1 (MQ=255)
                                                                                                                      tATTTCGGGTGGCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCaaa            <  7:271227/138‑1 (MQ=255)
                                                                                                                        tttCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAgg         <  2:107981/139‑1 (MQ=255)
                                                                                                                        tttCGGGTGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAgg         <  3:192108/139‑1 (MQ=255)
                                                                                                                           cGGGCGTCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGct       <  3:191091/138‑1 (MQ=255)
                                                                                                                           agggTGTCTTCATGC‑‑AAAAAACACACACGGATAGTCTGGAAAATCTAAACAATCCGTGTGCAGATATTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTAAACAAAACTCCGCATCGCAAAAGGct       <  2:96341/137‑1 (MQ=255)
                                                                                                                            gggTGTCTGCATGCAAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGct       <  8:14474/139‑1 (MQ=255)
                                                                                                                             ggTGTCAGCATGC‑‑AAAAAACACACCCGGATCGCCTCGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTATTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCtt    <  6:295086/139‑1 (MQ=255)
                                                                                                                              gtgtCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTc   >  7:151628/1‑139 (MQ=255)
                                                                                                                               tgtCTGCATGC‑‑AAAAAACACACCCGGATCGTCTGGTAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTcc  <  2:310032/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                
CTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCC  >  CP006881/3579647‑3579913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑