BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	121	G→C	5.6%	intergenic (–/‑2857)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	121	0	G	C	5.6%	13232.6 / 95.4	4722	intergenic (–/‑2857)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (1913/2320); new base C (4/335); total (1920/2802)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4798 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGACCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAGTTAATAGaa                                                                                                                                          <  2:207606‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  6:251640‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:346159‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGaa                                                                                                                                          <  4:78650‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTAGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          >  6:123629‑M1/17‑139 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  7:77124‑M1/127‑1 (MQ=255)

      gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTACGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  1:168610‑M1/129‑1 (MQ=255)

       cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  6:95180‑M1/130‑1 (MQ=255)

       cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGGCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    >  2:135349‑M1/10‑138 (MQ=255)

        ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  1:255308‑M1/130‑1 (MQ=255)

        ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  5:357040‑M1/130‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  5:42399‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  4:378831‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  5:323471‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTAAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  1:308930‑M1/132‑1 (MQ=255)

               aTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAg                                                                                                                           <  8:279821‑M1/138‑1 (MQ=255)

                  cATCCTCCACAGTGGGAGAGTGTGTTTTAGGGCCCAAATATTAATGTTACGGGTGAACCTTCTCAAATTTTCGTTGATTTAATAGAAACATTGCGGTAAAATTCGCACTAACTTAAAAGACCGTAAATCAAAAAAGCCa                                                                                                                        <  2:268322/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGATAGTGAGTTTTATGTCCAAAATATTAATGTTTCTGGTTAACCTTATCAAATTTTCGTTTATTTAATAGAAAAATAGCGTTAAAATTAGCATTAACTTAATAGAACGGAAATCAAAAAAGCCa                                                                                                                        <  3:364740/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  4:221828/121‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCa                                                                                                                        <  3:223081/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  5:253091/1‑139 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:303605/139‑1 (MQ=255)

                  cATACTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:250699/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  5:298283/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  3:7444/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  3:135587/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  3:29328/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  5:141935/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  5:385821/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  2:303803/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACATTATAAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCAct                                                                                                                      <  3:246043/139‑1 (MQ=255)

                    tcctcaAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACATTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCAct                                                                                                                      <  7:175992/139‑1 (MQ=255)

                    tcctcaAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATTAAAAAAGCCAct                                                                                                                      <  6:66277/139‑1 (MQ=255)

                     cctccAAAGTTGGAGTGGGATTTTTATGTCGAAAATATTAATGTTTCTGGTCAACCTTATCAAATTTTCTTTTATTAAATAGAAACATCGCGTTAAAATTTGCATTAAATTAATATAAGGTAAATGAAAAAAGCCActc                                                                                                                     <  8:304418/139‑1 (MQ=255)

                     cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                     >  6:189219/1‑139 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  4:288168/1‑132 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAAAGGAAATGAAAAAAGCCACTCTCat                                                                                                                 <  1:100201/139‑1 (MQ=255)

                                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                          >  5:212117/1‑139 (MQ=255)

                                 tagtgtgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAAATATATCAAATTTTCGTTTATTTAATACAAACATCGCGGTAAAATTAGCATTAAATAAATAGACCGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         <  4:194428/135‑1 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          <  2:40191/122‑1 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  3:386840/1‑139 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  7:118994/1‑139 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  5:114290/119‑1 (MQ=255)

                                       tagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATATAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    <  4:136434/137‑1 (MQ=255)

                                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  7:279821/139‑1 (MQ=255)

                                         gtTTTTTGGCGAAAATTTTATTGTTTCTGGTGAACCTTATAAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTAGCTGTAACTTAATAGAACGCAATTGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  8:330195/138‑1 (MQ=255)

                                         gtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  3:173926/138‑1 (MQ=255)

                                                  cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  1:132346/1‑139 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                >  3:106386/1‑113 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                                 <  3:271901/112‑1 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  7:22383/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  8:135893/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  2:72828/1‑137 (MQ=255)

                                                      aaTATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCgaga                                                                                    <  1:56539/139‑1 (MQ=255)

                                                       atatTATTTTTTCTTGTGAACCTTATCAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTAGCATTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  7:331413/139‑1 (MQ=255)

                                                            aaTGTTTCTGGCGAACCTTATCAAATTTTCCTTTATTAAATCGAAACAAAGCGTAAAAATTAGCATTAACTTAATACACCGGAACTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  8:38171/139‑1 (MQ=255)

                                                                ttgcTGGTTAACCTAATCAAATTTTCTTTTATTTAATCGAACCATAGCGGTAAATTTAGCAGTAACTAAATCGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  8:195861/135‑1 (MQ=255)

                                                                  tCTGTTTAACCTAATCAATTTTTCGTTGATTTAATAGAACCATAGCGGTAAAATTAGCAGAAACTTAATACACCGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         <  6:325169/138‑1 (MQ=255)

                                                                  tCTGGTGAGCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:68953/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  5:91248/1‑91 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  8:171773/136‑1 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:276475/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         <  6:358132/138‑1 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGATACCAACCTTTAGCGAGAATGACTTAATc                                                                         >  1:194624/1‑138 (MQ=255)

                                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         <  2:3343/105‑1 (MQ=255)

                                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                       >  2:361920/1‑139 (MQ=255)

                                                                      gttaaCCTTATCATATTTTCTTTTATTTAATAGAAAAATAGCGTTAAAATTAGAATTAACTTAATATAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTATCGAGAATGACTTAATCCTGTa                                                                    <  8:22367/136‑1 (MQ=255)

                                                                      gcgAACATTATAAAATTTTCTTTCATTTAATAGAACCTTCGCGGAAAAATTAGACTTAAATTAATAGACCGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  4:253631/137‑1 (MQ=255)

                                                                      gTGAACCTTTTAAAATTTTCGTTGATTTAATACAAAAAAAGCGTTAAAATTAGCAGTAACTTAAAAGACCGAAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGt                                                                     <  7:363810/138‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  6:398656/139‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  7:387770/139‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  6:294578/139‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  7:51304/139‑1 (MQ=255)

                                                                      cttaaCATTATCAAATTTTCGTTGATTTAATAGAAACAAAGCGGAAAAATTACCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  1:22414/136‑1 (MQ=255)

                                                                        gAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   >  4:104968/1‑122 (MQ=255)

                                                                         acccTAATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  5:195904/137‑1 (MQ=255)

                                                                         aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 >  3:45311/1‑139 (MQ=255)

                                                                          ccattATCTAATTTTGTTTTTTTTAATATAAAAATTGGGTTAAAAATAGCTTTAACTTAATAGAACGTAATTCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  4:314755/135‑1 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   >  2:240144/1‑116 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAAGAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTTGCTACCAACCGTTCGCGAGAATGCCTGAATCCTGTACAGCAAtc                                                            >  8:65898/1‑138 (MQ=255)

                                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                            >  3:19475/1‑137 (MQ=255)

                                                                                     ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                     >  7:146273/1‑139 (MQ=255)

                                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  5:169697/138‑1 (MQ=255)

                                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  6:212267/138‑1 (MQ=255)

                                                                                          gTTGATTAAATATAAACATACCGTAAAAATACCCATAAACTAAAAACACCGGAAAACAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                                 <  2:167394/138‑1 (MQ=255)

                                                                                                             aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                 <  3:337965/103‑1 (MQ=255)

                                                                                                               cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                            >  1:277825/1‑138 (MQ=255)

                                                                                                               cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                            >  3:317497/1‑138 (MQ=255)

                                                                                                               cGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCa                           <  8:250011/139‑1 (MQ=255)

                                                                                                                ggTAAAATTAGCAGTAACTTAATAGATCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGCCTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCa                           >  8:357885/1‑138 (MQ=255)

                                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   >  6:157799/1‑81 (MQ=255)

                                                                                                                  tAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                         >  8:110792/1‑138 (MQ=255)

                                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATaa                      >  4:41819/1‑139 (MQ=255)

                                                                                                                         aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                 >  7:203303/1‑139 (MQ=255)

                                                                                                                         aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                 >  1:391984/1‑139 (MQ=255)

                                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                         >  1:9504/1‑76 (MQ=255)

                                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  4:16474/1‑139 (MQ=255)

                                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  1:365829/1‑139 (MQ=255)

                                                                                                                                    aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCACCTTCAAATAAGTTATTTGCCTTGttt      >  1:335662/1‑139 (MQ=255)

                                                                                                                                        gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc  >  8:402592/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  pSIJ663_pHT315_araE_xy/1‑259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`