BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	126	G→T	5.2%	intergenic (–/‑2852)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	126	0	G	T	5.2%	12225.8 / 70.7	4846	intergenic (–/‑2852)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (2218/1852); new base T (13/422); total (2239/2607)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4964 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACA  >  pSIJ663_pHT315_araE_xy/1‑264

atgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACgg                                                                                                                                            <  7:144655‑M1/126‑1 (MQ=255)

   cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGATTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                         >  2:209692‑M1/10‑138 (MQ=255)

    ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                        <  7:173286‑M1/130‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAATTTTTCTTTGATTTAATAGAAACATAGCTGTAAAATAAGCAGTAAATTAATAGAACGGAAATGa                                                                                                                                      <  4:191017‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  2:402‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  6:165527‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  1:1968‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  3:293769‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                      <  1:95324‑M1/132‑1 (MQ=255)

      caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGGTTCTGGTGAACCTTATCAAATTTTCTTTGATTTAATAGAAACATATCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGa                                                                                                                                      <  1:224630‑M1/132‑1 (MQ=255)

            tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGTCCCTTATCAAATTTTCTTTGATTAAATATAAACATCGCGGAAAAATTAGCCGTAACTTAATACAACGGAAATGAAAAAAGc                                                                                                                               <  4:406136/139‑1 (MQ=255)

            tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                               >  3:389692/1‑139 (MQ=255)

              cctcctccAAAGTGGGAGAGTGAGTTTTATGTCTAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             <  7:203999/137‑1 (MQ=255)

              cagccaccAACGTGGGAGAGTGATTTTAATGTCCCAAATATAAATGTTTCGGGTCAACCTTATCAAATTTTCGTTGATTTAATCCAAACAAAGCGGAAAAATTAGCAGTAACTAAAAAGAACGGAAATTAAAAAAGCCa                                                                                                                             <  8:379751/133‑1 (MQ=255)

              cATCCTCCAAAGTTGGAGTGTTAGTTTTATGTCGAAAATATTAATTTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATACAAACATAGCGTTAAAATTAGCAGTAAATTAATACAACGGAAATCAAAAAAGCCa                                                                                                                             <  2:130041/139‑1 (MQ=255)

              cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             <  7:76669/139‑1 (MQ=255)

              cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             >  7:48092/1‑139 (MQ=255)

              cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             <  2:362011/139‑1 (MQ=255)

              cATCCTCAAAAGTTGTCTAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACATTATAAAATTTTGTTTTATTAAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAAAAGAACGCAAATGAAAAAAGCCa                                                                                                                             <  7:278051/139‑1 (MQ=255)

              cATACTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             <  7:250699/139‑1 (MQ=255)

               aTCCTCCAAAGTTGGAGTGTGTTTTTTATGTCGCAAATATTAATGTTTGTGGTGAACATTATCAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCATTAACTTAATAGAACGGAAATTAAAAAAGCCAc                                                                                                                            <  5:44060/139‑1 (MQ=255)

                tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                           >  1:306289/1‑139 (MQ=255)

                 cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                          >  5:297739/1‑139 (MQ=255)

                 cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                          >  1:335097/1‑139 (MQ=255)

                    caaaaGTTGGAGAGTGTTTTTTATGTCGAAAATAATAATGTTTCTGGTGAACATTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATTAAAAAAGCCACTCTCa                                                                                                                       <  4:220130/137‑1 (MQ=255)

                     cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                             >  4:288168/1‑132 (MQ=255)

                     cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAAAGGAAATGAAAAAAGCCACTCTCat                                                                                                                      <  1:100201/139‑1 (MQ=255)

                      aaaTTTGGAGAGTGAGTTTTATTACGAAAATATTAATGTTTGTGGTTAACCTTATAAATTTTTCGTTTATTTAATACAAAAATTGGGTTAAAATAAGCTTTAATTTAAAAGAACGCAAATAAAAAACGCCACTCTCata                                                                                                                     <  4:252064/139‑1 (MQ=255)

                            ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                               >  5:212117/1‑139 (MQ=255)

                            ggAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTa                                                                                                                <  8:11001/138‑1 (MQ=255)

                             gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTTATTAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  7:330195/1‑139 (MQ=255)

                             gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  7:118994/1‑139 (MQ=255)

                             gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                              >  6:65714/1‑139 (MQ=255)

                                    agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  7:223314/139‑1 (MQ=255)

                                    agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  4:312362/139‑1 (MQ=255)

                                    agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                        >  5:386120/1‑138 (MQ=255)

                                     gtTTTTTGTCGCAAATATAATTGTTTCTGTTTAACATTATAATATTTTGTTTTTTTTATTTGAAACATTTCTTTAAATTTAGATGTACATTAATAGAAGGTAAATGAATATAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       <  8:246496/138‑1 (MQ=255)

                                          aTGTCGGAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                 >  3:104538/1‑139 (MQ=255)

                                                cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                           >  6:358329/1‑107 (MQ=255)

                                                cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                             >  2:332769/1‑137 (MQ=255)

                                                cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                             >  8:190347/1‑137 (MQ=255)

                                                   atatTATTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAAAAGAAAAATAGCGTTAAAATTAGAAGTAACTTAATACAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  3:243353/139‑1 (MQ=255)

                                                   atatTAATTTTTCTTTTTAACCTTATAAATTTTTCTTTTATTTAAATTAAAATTAGGGGTAATATTAGCTGTAAATTAATAGAACGTAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  5:71615/139‑1 (MQ=255)

                                                   atatTAATGTTTCTGGTTAACCTTATCAATTTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  8:164474/139‑1 (MQ=255)

                                                   atatTAATGTTTCTGGTGAACCTTTTCAATTTTTCGTTGATTTAATAGAAATATCGCTTAAATATATGCTGTATCTAAATACAACGAAAAAGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  5:251640/139‑1 (MQ=255)

                                                         aTGTTTCGGGTGAACCTTATCAAATTTTCGTTGAATTAAAAGAAACATAGCGGAAAAATTCGCAGTAACTTAATAGAACGGAAATGAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                   <  2:267997/138‑1 (MQ=255)

                                                            tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                <  1:393/138‑1 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                        <  5:324549/128‑1 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  3:317712/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  1:255138/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  3:130665/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  4:102347/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  7:301125/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  5:94464/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  5:276475/1‑139 (MQ=255)

                                                              tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                             >  1:363247/1‑139 (MQ=255)

                                                               cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                            >  8:6570/1‑139 (MQ=255)

                                                               cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                            >  8:354177/1‑139 (MQ=255)

                                                                  tttaaCATTATAAAATTTTCTTTTATTTAATACAAACAAAGCTGAAAATTTCGAAGTAAGTTAATACAACGGAATTTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  4:15568/136‑1 (MQ=255)

                                                                  gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTAGAAGTAACTTAATAGAACGCAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  3:365535/139‑1 (MQ=255)

                                                                  gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                           <  6:135801/137‑1 (MQ=255)

                                                                  gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  2:240114/139‑1 (MQ=255)

                                                                  gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  1:276994/139‑1 (MQ=255)

                                                                  gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  7:387770/139‑1 (MQ=255)

                                                                  gTGAACCTTATAAATTTTTTGTTGATTTAAAACAAACAAAACGTAAAAATTAGAATTAACTAAATACAACGGAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  3:220119/139‑1 (MQ=255)

                                                                   tGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                        <  1:132557/139‑1 (MQ=255)

                                                                     aaCCTTATCAAATTTTCGTTGATTTAATTGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  3:170906/139‑1 (MQ=255)

                                                                     aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      >  3:45311/1‑139 (MQ=255)

                                                                     aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  2:164664/139‑1 (MQ=255)

                                                                     aaCCTAATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAAATACCAGTAACTAAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  8:272977/139‑1 (MQ=255)

                                                                          tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                         <  6:192795/131‑1 (MQ=255)

                                                                          tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                 >  5:171372/1‑139 (MQ=255)

                                                                                  tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         >  3:273120/1‑139 (MQ=255)

                                                                                   ttCGTTTTTTTAATACAAACATACCGGTAAAATTAGCCGAAACTTAATAAACCGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  7:362099/138‑1 (MQ=255)

                                                                                   ttCGTTTATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  5:114472/138‑1 (MQ=255)

                                                                                   ttCGTTTATTTAATAGAAAAATTGCTTTAAAAATAGCTGTAAGTTAATATAACTGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  4:37852/138‑1 (MQ=255)

                                                                                   ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  8:131506/138‑1 (MQ=255)

                                                                                   ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  3:194068/138‑1 (MQ=255)

                                                                                   ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                         <  4:130665/138‑1 (MQ=255)

                                                                                    tctttGATTTAATAGAAACATAGCGGAAAAATTAGCAGTAAATTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                        <  6:93953/135‑1 (MQ=255)

                                                                                                  gAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcg                                         >  1:57036/1‑139 (MQ=255)

                                                                                                    aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                >  6:397704/1‑98 (MQ=255)

                                                                                                       aTAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                                    <  4:345725/139‑1 (MQ=255)

                                                                                                       aTAGCGGTAAAATTACCAGTAACTAAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                                    <  1:163419/139‑1 (MQ=255)

                                                                                                         aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                       >  8:401130/1‑70 (MQ=255)

                                                                                                         aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                           >  5:357387/1‑98 (MQ=255)

                                                                                                         aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                      <  3:337965/103‑1 (MQ=255)

                                                                                                         aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                  >  2:300200/1‑139 (MQ=255)

                                                                                                             gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                              >  4:7479/1‑139 (MQ=255)

                                                                                                                aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATa                            <  5:195508/138‑1 (MQ=255)

                                                                                                                             cTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTg              >  8:3244/1‑139 (MQ=255)

                                                                                                                                    gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAATTTATTTGCCTTGTTTTcgc       >  6:326953/1‑139 (MQ=255)

                                                                                                                                     aaCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc       >  8:222948/1‑138 (MQ=255)

                                                                                                                                       cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                       <  6:215497/104‑1 (MQ=255)

                                                                                                                                       cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  2:68666/1‑139 (MQ=255)

                                                                                                                                       cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  8:299931/1‑139 (MQ=255)

                                                                                                                                       cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    >  6:295361/1‑139 (MQ=255)

                                                                                                                                       cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa    <  6:261093/139‑1 (MQ=255)

                                                                                                                                        ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc   <  3:73499/139‑1 (MQ=255)

                                                                                                                                         gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa  <  5:146523/139‑1 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACA  >  pSIJ663_pHT315_araE_xy/1‑264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`