BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	137	A→C	5.4%	intergenic (–/‑2841)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	137	0	A	C	5.4%	12054.2 / 102.7	4773	intergenic (–/‑2841)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (2230/2168); new base C (21/349); total (2256/2517)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4932 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

tacATCCTCCAAAGTTGCTGAGTGAGTTTTATGTCGAAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTAAATAGAAACATAGCGTTAAATTTCGCTTTAACTAAATAGACCGCAAATCAAAAAAGc                                                                                                                                          <  2:366982/137‑1 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  1:160180/1‑139 (MQ=255)

  caacctccAAATTTGGAGAGTGAGTTTTATGTCGCAAATATAAATGTTACTGGTCAACCTTATCAAATTTTCGTTGATTATATAGAAACATAGCGTTAAAATTTGCATTAACTTAATACAACGCAAATGAAAAAAGCCa                                                                                                                                        <  1:13079/136‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGTGTGATTTTTTTGTCGAAAATATTAATGTTTCTGGTGAACCTTTTAAATTTTTGGTTGATTTAATATAAAAATAGCGGTAAAATTAGCAGAAAATAAATAGAACGGAAATTAAAAAAGCCa                                                                                                                                        <  2:33791/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCa                                                                                                                                        <  3:223081/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  6:5618/1‑139 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:222901/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACACCGTAAATGAAAAAAGCCa                                                                                                                                        <  1:224147/139‑1 (MQ=255)

  cATCCTCAAATGTTGTAGAGTGAGTTTTTTGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGACCGTAAATGAAAAAAGCCa                                                                                                                                        <  4:113503/139‑1 (MQ=255)

  cATCCTCAAAAGTTGGAGAGTTAGTTTTATGTCGAAAATATTAATGTTTCTGGTTAACCTTATAAATTTTTCGTTTATTAAATACAAACATAGCGGTAAAATTAGCATTAACTAAATAGAACGTAAATCAAAAAAGCCa                                                                                                                                        <  4:7505/139‑1 (MQ=255)

  cATACTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:250699/139‑1 (MQ=255)

    tcctccAAAGTTGGAGAGTTAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAAAAGAAAAATAGCGTTAAAATTACCAGTAAATTAATAGAACGTAAATGAAAAAAGCCAct                                                                                                                                      <  7:170953/139‑1 (MQ=255)

    tcatcaaaaGTTGGAGAGTTAGTTTTATGTCGCAAATATTAATGTTTATGGTGAACATTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                                      <  8:247891/133‑1 (MQ=255)

       tccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGATATGAAAAAAGCCActct                                                                                                                                    >  4:348581/1‑138 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                                                 <  5:356445/139‑1 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGACACTCTCa                                                                                                                                  >  3:299009/1‑138 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAAAGGAAATGAAAAAAGCCACTCTCat                                                                                                                                 <  1:100201/139‑1 (MQ=255)

          aaaGTTGGAGAGTTAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                                <  5:72299/139‑1 (MQ=255)

          aaaGTGGGAGAGTGAGTTTTAGGGCGCAAATATTAATGTTTCTTGTGAACCTTATCAAATTTGCGTTGATTAAATAGAAACAAAGCGGTAAAATTCGCAGAACCTTAATATAACGGAAATGAAAAACGCCACTCTCata                                                                                                                                <  8:298709/139‑1 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  5:248915/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  5:9331/1‑139 (MQ=255)

                     ttgagtTTTATTTCCAAAATATAAACCTTGCTGGTTAACCTTATCAAATTTTGGTTGATTTAATATAAACATAGCGGTAAAATTCGCAGAAACTTAAAACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGc                                                                                                                      <  5:171690/137‑1 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  4:204388/1‑139 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:116026/117‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  5:386120/1‑138 (MQ=255)

                         gtTTTATGTCGCAAATATTAATGTTTCTGGGGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  4:294933/138‑1 (MQ=255)

                         gtTTTATGTCGCAAAATTTAATGTTTCTGGTGAACCTTATAAAATTTTCTTTTATTTAAAAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  7:75601/138‑1 (MQ=255)

                              aTGTCGGAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                            >  3:104538/1‑139 (MQ=255)

                              aTGTCGAAAATTTTAATTTTTGGGGTGAACATTATAATATTTTCGTTTTTTTAATACAAACATCCGGTTAAAAATAGCATTAATATATTACAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                                             <  2:168757/138‑1 (MQ=255)

                               tgcccaaaaTATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGTTAAAATTAGCAGAAACTTAATAGACCGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                                            <  3:81080/132‑1 (MQ=255)

                                gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACGtt                                                                                                            >  5:44110/1‑137 (MQ=255)

                                  cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  8:110466/1‑139 (MQ=255)

                                   gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        <  8:192616/138‑1 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGATACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  1:254055/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGc                                                                                                        >  4:17279/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  3:131264/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  5:295816/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  1:19460/1‑137 (MQ=255)

                                       tttttACTGTTTATGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATACTGGAAAAATAAGCAGTAACTTAATTTAAGGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  2:69717/136‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTCTCAAATTTTCGTTGATTTAAAAGAAACAAAGCGGTAAAATTCGCAGAAACTTAAAACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  4:136470/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  6:87219/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATAAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  4:234020/139‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTTTTTTAATATAAACATAGGGGTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  3:318530/138‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACtt                                                                                             <  7:139120/139‑1 (MQ=255)

                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  7:330422/138‑1 (MQ=255)

                                                  tgtGGTGAACCTTATAAAATTTTCGTTGATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTTAATAGACCTGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  8:65997/136‑1 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAACAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  8:355279/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGATTGACTTAATcc                                                                                        >  3:37287/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:62053/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  7:301125/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:395577/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGATACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  1:367907/1‑139 (MQ=255)

                                                   cTGGTGACCCTTATCAATTTTTCGTTGATTTAATAGACACATTGCGGGAAAGTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCGTATGCTATTGGCTACGAACCTTTAGCGAGAATGCCTTAATcgt                                                                                       >  7:388835/1‑137 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                       >  5:114255/1‑139 (MQ=255)

                                                    tGGTCAACCTTATCAAATTTTCGTTGATTTAATACAAACATAGCGTAAAAATTAGCAGTAACTTAATACACCGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  6:258583/139‑1 (MQ=255)

                                                     ggTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  6:325944/138‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  1:9634/139‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                                      <  6:135801/137‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  6:294578/139‑1 (MQ=255)

                                                      gTGAACATTATCAAATTTTCGTTGATTTAATATAAACATCGCGTTAAAATTACCAGAAACATAATAGACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  1:336564/139‑1 (MQ=255)

                                                       taaaCCTTATAAAATTTTCTTTTTTATTATACAAACTTAGCGTAAAATTTTCCAGTAACTTAATAGACGCAAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  4:268066/136‑1 (MQ=255)

                                                         aaCCTTAAAAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAAAATAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  4:317716/139‑1 (MQ=255)

                                                         aaCCTAATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAAATACCAGTAACTAAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  8:272977/139‑1 (MQ=255)

                                                          acgttATAAAATTTTCGTTTATTTAATATAAACATCGCGGTAAAATTAGCAGTAATTTAATAGAACTTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  8:165433/135‑1 (MQ=255)

                                                               aTCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  3:249863/1‑138 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  3:19475/1‑137 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            >  3:172764/1‑137 (MQ=255)

                                                                    aTTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAGTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGAACAGCCATACAGGAc                                                                      >  2:242730/1‑139 (MQ=255)

                                                                     ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                                     >  5:211531/1‑139 (MQ=255)

                                                                      tttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                           <  2:27329/132‑1 (MQ=255)

                                                                        tCGTTTATTTAATATAAACATAGCGGAAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                                   <  2:215814/138‑1 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  3:276625/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  7:20230/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  8:402934/1‑138 (MQ=255)

                                                                                           aTAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                                               <  3:346159/139‑1 (MQ=255)

                                                                                                 gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGTATACCAACATTTAGCGAGAATTACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACATcctg                                         >  5:194377/1‑136 (MQ=255)

                                                                                                    aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                              <  5:146974/83‑1 (MQ=255)

                                                                                                                aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  5:324850/1‑139 (MQ=255)

                                                                                                                 cTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTg                         >  8:3244/1‑139 (MQ=255)

                                                                                                                      tAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc                    <  7:362569/139‑1 (MQ=255)

                                                                                                                        gAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAATTAAGTTATTTGCCTTGTTTTcgc                  >  2:136008/1‑139 (MQ=255)

                                                                                                                        gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                  >  3:58302/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         >  8:130310/1‑65 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  6:168896/1‑73 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               <  8:22383/139‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  4:385108/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  2:40400/1‑139 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               <  6:356566/139‑1 (MQ=255)

                                                                                                                           cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  3:399257/1‑139 (MQ=255)

                                                                                                                            ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTGCAAATAAGTTATTTGCCTTGTTTTCGCGat               >  1:306645/1‑137 (MQ=255)

                                                                                                                            gaaactGAAACAAGCCACGCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc              <  6:217158/134‑1 (MQ=255)

                                                                                                                             taaaTCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  7:47364/138‑1 (MQ=255)

                                                                                                                             gAAATGAAAAAAGCCACTCTCATCTGCTATTGGCTACCAACCTTTAGCGAGACTGAAGTAATCCTGTACAGCCATACACGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  6:389569/139‑1 (MQ=255)

                                                                                                                             gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  6:194445/139‑1 (MQ=255)

                                                                                                                             gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  8:381895/139‑1 (MQ=255)

                                                                                                                              aaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACaa            <  5:272537/139‑1 (MQ=255)

                                                                                                                               aaagAAAAAATCAACTCTCATATGCTATTTGCTACCAACCTTTATCGAGAATGACTTAATCGAGTAGATCCATACAGGACTTCGACTTATAAGTCGCTCGTACTTCAATTAAGTTATTTGCCTTGTTTTCGCGAACAAg           <  2:306344/136‑1 (MQ=255)

                                                                                                                               aaTCAAACAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAAAGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg           <  4:43317/139‑1 (MQ=255)

                                                                                                                                aTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAgg          >  1:277505/1‑139 (MQ=255)

                                                                                                                                   aaaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCt        >  7:276644/1‑138 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`