BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,743	A→T	5.0%	intergenic (+28/‑128)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,743	0	A	T	5.0%	10579.9 / 64.5	3348	intergenic (+28/‑128)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (248/2867); new base T (202/6); total (474/2874)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3434 total aligned reads displayed.

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3605‑3882

 aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGa                                                                                                                                             <  1:35272/138‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  8:32572/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  6:44852/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:105500/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:400571/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  4:127783/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  7:93389/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  2:173134/139‑1 (MQ=255)

        tcaAGCAATGAAACAAGCCAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  1:184932/139‑1 (MQ=255)

          aaGCAATTAAACACGCCAAATTAATCAATTTAAGTACCATTACTTATGATCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  7:162073/139‑1 (MQ=255)

          aaGCAATGAAACACGCCTAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:266078/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  7:267183/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  6:318782/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:235204/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  1:10001/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  1:277103/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  8:359800/139‑1 (MQ=255)

             cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGtt                                                                                                                                 >  8:75051/1‑138 (MQ=255)

                  aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  1:247557/139‑1 (MQ=255)

                  aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                            <  5:405803/138‑1 (MQ=255)

                      acGCCAAAGTATACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAATGGGa                                                                                                                       <  2:361345/139‑1 (MQ=255)

                       cgcgAAAGTAAACAATTTTAGTAACATTACTTATGATGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  3:91933/139‑1 (MQ=255)

                          cAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGATATGTAAACGTTAATAAATGGAAAgg                                                                                                                   >  5:374856/1‑139 (MQ=255)

                               tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  5:68550/139‑1 (MQ=255)

                               tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  8:125389/139‑1 (MQ=255)

                                   cAATTTAACTACCATTACTTATGAGCAAGTATTGTCTATATTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                          <  6:35311/139‑1 (MQ=255)

                                     aTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                                        <  2:103921/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:353832/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:249727/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:219205/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  6:379275/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:133002/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:192854/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  4:283957/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  8:221760/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:156536/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:150910/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTTTAATTTAAACATTAATAAATGGCATGGTGATaaaactgt                                                                                                       >  2:13269/1‑134 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  8:276722/139‑1 (MQ=255)

                                         aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  8:199225/138‑1 (MQ=255)

                                          aGTACCATTACTTTTGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACTTGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                   <  2:93522/139‑1 (MQ=255)

                                           gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  3:316494/139‑1 (MQ=255)

                                              ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  3:151963/138‑1 (MQ=255)

                                              ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  5:320984/138‑1 (MQ=255)

                                              ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  4:43649/138‑1 (MQ=255)

                                                 ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTATTTTTTTAAGTTTAAACTAAATAAAGGGTATGGTAATAATATATTTTATataataa                                                                                            >  8:247162/1‑138 (MQ=255)

                                                       aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  6:178018/137‑1 (MQ=255)

                                                       aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  6:206302/137‑1 (MQ=255)

                                                         gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAGTTACACGTTACTAAAGGGAATGGTGATAAATTTTTTTATATACTACTgctgtct                                                                                    >  2:65733/1‑133 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  5:92436/139‑1 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTAAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  4:30827/139‑1 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTAATTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  1:325658/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  3:185959/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                               <  4:25399/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGTAATTTACAAGTTACTAAAGGGAATTGAGATAAATTATTATATATTATACTGACAGCTTCCaa                                                                               >  7:19288/1‑139 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTAATTTTGTTAATTACACGTTTGTAAAGGGGATGGGGATATATTATTTTATATTATAAAGTCAGCTTCCa                                                                                >  2:283610/1‑138 (MQ=255)

                                                               gacaggTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  7:334317/134‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  4:217480/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  6:285939/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAAATCTATGAGTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  8:164522/139‑1 (MQ=255)

                                                                tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAACGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTCCTGACAGCTTCCaaga                                                                             <  5:271020/139‑1 (MQ=255)

                                                                tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  6:76137/139‑1 (MQ=255)

                                                                tATTGTCTATTTTTAATACTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  7:357219/139‑1 (MQ=255)

                                                                 aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  4:155138/138‑1 (MQ=255)

                                                                  ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                           <  1:48095/139‑1 (MQ=255)

                                                                           tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  2:391432/138‑1 (MQ=255)

                                                                               aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              >  1:12323/1‑91 (MQ=255)

                                                                               aTAGTTATCTATTATTTAACGGGAGGAAATAATTATATTAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTccc                                                              <  2:205405/139‑1 (MQ=255)

                                                                                  gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                           <  5:111328/139‑1 (MQ=255)

                                                                                     aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGAAAGTTTCACGTTACTAAATGGTATTGAGATAAAATATTAGATATACTACTGACAGCTTACAATAAGCTAAAGAGGTCCCAAgcgc                                                        >  8:297462/1‑139 (MQ=255)

                                                                                            attTAACGGGATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  1:305834/138‑1 (MQ=255)

                                                                                            attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  3:364226/138‑1 (MQ=255)

                                                                                             tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGCCAGCATCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  2:23727/138‑1 (MQ=255)

                                                                                             tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  3:122034/138‑1 (MQ=255)

                                                                                             tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                 <  1:2176/138‑1 (MQ=255)

                                                                                               tAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                               <  5:298742/138‑1 (MQ=255)

                                                                                                aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTAAACCGTAATAAAGGGTATGGAGATTAATTATTATATATAATAAAGACAGCATACAAGAAGCGAAAGAGGTGCCAAGCGCCGACCGGGTAt                                             >  8:52143/1‑139 (MQ=255)

                                                                                                 tcGGGAGGTAATAATTCTATGAGTCGCTTTTTTAATTTTGGAAAGTTACACGTTACTATAGGGATTGGACATATATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAAt                                             <  6:116559/137‑1 (MQ=255)

                                                                                                 aCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                             <  7:224623/138‑1 (MQ=255)

                                                                                                 aCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                             <  7:72465/138‑1 (MQ=255)

                                                                                                 aCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                             <  7:293560/138‑1 (MQ=255)

                                                                                                  cGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGGTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTACAAGAAGCTAAAGAGGTCCCTATCGCCGACGGGGAAttt                                           >  6:141734/1‑139 (MQ=255)

                                                                                                                    tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  7:381122‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                            <  4:37973‑M1/136‑15 (MQ=255)

                                                                                                                    tGAGTCGCCTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGGCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaag                          >  7:40413‑M1/1‑122 (MQ=255)

                                                                                                                    tGAGCCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                            <  8:388331‑M1/136‑15 (MQ=255)

                                                                                                                     gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  4:189479‑M1/138‑18 (MQ=255)

                                                                                                                     gAGTCGATTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGCAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  5:134005‑M1/138‑18 (MQ=255)

                                                                                                                      aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  1:387181‑M1/139‑20 (MQ=255)

                                                                                                                      aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAAACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  6:240373‑M1/139‑20 (MQ=255)

                                                                                                                            ttttttATATTTGTAAAGTTACACGTTAAAATAGGGTATTGAGTTTAATTTTTTTATAAATTTATGACAGCTTGCAAGATGCTAATGAGGTCCCTAGCGCCTACGGGGATTTTG‑‑‑‑GGGG‑ACATTGAAAAAGGAAgtgtat            <  2:320443/139‑1 (MQ=255)

                                                                                                                            ttttttAAATTTGGAAATTTACACTTTACTAAAGGTAAGGGAGATAAATTATTAGATATACTACTGACCGCTTCCACGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggacgagtat                 <  3:290924‑M1/139‑26 (MQ=255)

                                                                                                                            ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  3:338715‑M1/139‑26 (MQ=255)

                                                                                                                             tttttAAATTTGTAAAGTTACATGTTAGTAGAGGGAATGGAGATAATTTATTAGATATAATACTGACAGCTTCCAAGATGCTTACGAGGTCCCTAGGGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  4:79613‑M1/138‑26 (MQ=255)

                                                                                                                             tttttAAATTTGGAAAGTTAAACTTAACTAAAGCTAATTTAGATAAATTATTAGATATACTACAGACAGCTTCCAATAAGATAAACAGGTCCCTAGCGCCTACGGGTAATTTGgggtacattgaaaaaggaagagtat                 <  3:58240‑M1/138‑26 (MQ=255)

                                                                                                                              ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  7:317404‑M1/137‑26 (MQ=255)

                                                                                                                              ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  3:2103‑M1/137‑26 (MQ=255)

                                                                                                                              ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  4:346439‑M1/137‑26 (MQ=255)

                                                                                                                               tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATTAATTATTAGATATACTAGTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaaggaagagtatga               <  1:73627‑M1/138‑28 (MQ=255)

                                                                                                                                   aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt          <  7:244614‑M1/139‑33 (MQ=255)

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  pSIJ663_pHT315_araE_xy/3605‑3882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`