BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,625	A→C	5.8%	K252T (AAG→ACG)	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,625	0	A	C	5.8%	8220.6 / 73.2	3015	K252T (AAG→ACG)	ampR
Reads supporting (aligned to +/- strand): ref base A (1645/1099); new base C (14/228); total (1669/1346)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3076 total aligned reads displayed.

AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  pSIJ663_pHT315_araE_xy/4488‑4763

 gccTGGCTGTAGGCTGATTAAGTTGCAGGACCACTTCTGCGCTCGGACCTTCCGTCTGGCTGGTTTATTGCTTATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGTGGCCAGATGGTAAg                                                                                                                                           <  6:107871/136‑1 (MQ=255)

 gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  6:90935/138‑1 (MQ=255)

  aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  1:126643/139‑1 (MQ=255)

  aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  7:271709/139‑1 (MQ=255)

  aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  5:149201/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCTGTGAGCGTGGGTCTCGCGGTATCATTGAAGCACTGGTGCCAGATGGTAATccc                                                                                                                                        >  5:172760/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCTGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  8:245576/138‑1 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  4:94878/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  1:39124/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  4:269333/1‑139 (MQ=255)

   cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  4:234683/1‑139 (MQ=255)

    tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  4:55852/135‑1 (MQ=255)

    tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCt                                                                                                                                       >  8:74516/1‑139 (MQ=255)

    tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCt                                                                                                                                       >  2:187845/1‑139 (MQ=255)

    tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTGTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTCGGGCCAGATGGTAAGCCCt                                                                                                                                       >  7:368097/1‑139 (MQ=255)

     ggatggaGGCGGATACAGTTGCAGGAGGTCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATGTGGAGCCGGTGAGCGTGCTTCTCGCGGTATCTTTGCAGCACTGGGGCCAGAAGGTAAGCCCTc                                                                                                                                      <  4:136642/139‑1 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           >  7:250202/1‑134 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           >  6:272053/1‑134 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  7:168/139‑1 (MQ=255)

     ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  5:218152/1‑139 (MQ=255)

      gattgaGGCGGACAAATTTGCAGGACCACTGCTGAGCTCGGCCCTTCCGGCTGGCTGGTTTATTGATTATAAATCTGGAGCCGGTGAGCGTGGCTCGCGCGGTATCATTTCAGCACTGGGGCCAGATTGTAAGCCCTc                                                                                                                                      <  6:352554/138‑1 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTTATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  6:383474/138‑1 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     >  4:390862/1‑139 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  4:368613/138‑1 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCGCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  1:151700/138‑1 (MQ=255)

      gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCGCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  2:63319/138‑1 (MQ=255)

       atggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTTCTGATAAATCTGGAGAAGGTGAGCGTGTGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCACTccc                                                                                                                                    >  8:330162/1‑139 (MQ=255)

        tggaGGCCGATAAACTTCCACGACCACTTCTGCGCTCGCCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCCGCACTGGGGCGAGATGGTAATCCCTCCCg                                                                                                                                   <  8:356403/139‑1 (MQ=255)

          gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGTCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 <  3:316102/139‑1 (MQ=255)

          gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGt                                                                                                                                  <  7:396240/138‑1 (MQ=255)

          gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 <  4:105161/139‑1 (MQ=255)

            ggCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCTGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTAGCGGTATCATTGCAGCACTGTGTCAAGATGGTAAGCACTCCCGTATc                                                                                                                               >  5:314482/1‑139 (MQ=255)

             gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  3:145644/1‑139 (MQ=255)

               ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  1:24606/139‑1 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  2:222558/1‑139 (MQ=255)

                gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGGTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGGAg                                                                                                                           >  7:223157/1‑139 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 >  1:76983/1‑123 (MQ=255)

                          cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  7:317520/1‑139 (MQ=255)

                                 aCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGTTAAGCCCTCCCGTATCGTAGTTATCTACACTACggg                                                                                                           >  3:125555/1‑138 (MQ=255)

                                       gcgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAAGCTAAGCCCTCCCGTATCGTAGTTATCTACACTACGGGGACTCAg                                                                                                    >  5:240288/1‑139 (MQ=255)

                                        cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCg                                                                                                                                   >  5:343290/1‑107 (MQ=255)

                                        cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  7:188856/1‑139 (MQ=255)

                                        cgcTCGGCCCTGCCGGGGGGCGGGTTTATTGCTTATAAATTGGGCGCGGGTGAGGGTGGGGCTCGGGTTATCTTTCCCGCCCTGGGGCCATATGGTAAGCCCTCCCGTATCGTCGTTATCTACACGACGGGGAGTCAgg                                                                                                   <  6:139409/139‑1 (MQ=255)

                                          cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGc                                                                                                  >  7:343201/1‑138 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTTCAGCACTCGGGCCAGATGGTAAGCCCTCCCTTATCGTAGTTATATACACGACGCGGAGTCAGGCAAc                                                                                               >  4:155098/1‑138 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  3:379398/1‑99 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                >  2:23953/1‑105 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              <  8:302956/107‑1 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  8:217732/1‑138 (MQ=255)

                                             ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  2:402628/1‑138 (MQ=255)

                                               cccTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTAt                                                                                            >  7:52989/1‑139 (MQ=255)

                                                     cggcggGCTGGTTTATTGCTGATAAATCGGGAGCGGGTGCGCGTGGGTCTCGCGGTATCTTTGCAGCCCTGGGGCCAGATGGTAACCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  1:32824/139‑1 (MQ=255)

                                                          ggggggTTTATTGCTTATAACTCTGGAGCCGGTGAGGGTGGGGCGCGCGTTATCATTCCAGCACGGGGGCCAGAGGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaa                                                                                 <  8:175029/135‑1 (MQ=255)

                                                          ggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGa                                                                                  >  8:116978/1‑138 (MQ=255)

                                                          ggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGa                                                                                  >  5:77481/1‑138 (MQ=255)

                                                           gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  4:333496/85‑1 (MQ=255)

                                                            ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCCCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTTTGGATGAACGaaa                                                                                >  6:5506/1‑138 (MQ=255)

                                                            ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  2:332950/1‑92 (MQ=255)

                                                                  tATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCTGGCAACTTTGGATGAAGGAAATAGAct                                                                          >  2:359323/1‑137 (MQ=255)

                                                                         gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  5:403227/1‑138 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  1:353540/1‑67 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  1:269239/1‑137 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  1:295473/1‑137 (MQ=255)

                                                                          aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  7:294231/1‑137 (MQ=255)

                                                                              ttCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTgaga                                                               >  5:192432/2‑137 (MQ=255)

                                                                                 gggAGCCGGTGAGCGTGGGGCGCGCGGTTTCATTCCCCACCGGGGGCCAGATGGTACGCCCCCCCGTCTCGTCGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                              <  7:88860/134‑1 (MQ=255)

                                                                                  ggAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTg                                                         >  3:83350/1‑139 (MQ=255)

                                                                                       gggggcgCGTGGGGCCCGCGGTACCATTGCACCACTGGGGCCAGATGGTAAGCCCTCCCGAATCGTAGTAATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  2:137052/133‑1 (MQ=255)

                                                                                       gggTGCGGGTGGGGCTCGGGGTATCAGTCCCGCACGGGGGCCAGATGTTACGCCCGCCCTTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  2:2978/138‑1 (MQ=255)

                                                                                       gggTGCGGGTGGGGCGCGGGGTATCATGCCAGCACGGGGCCCAGTTGGAACCCCCTCCCTTATCGTATTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  5:126233/138‑1 (MQ=255)

                                                                                         gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  7:12154/1‑139 (MQ=255)

                                                                                          tGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAAAGAGAGAACGCTCAGATAGGTCCCTCACt                                                  >  7:14833/1‑138 (MQ=255)

                                                                                              cGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAAAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              >  7:71543/1‑138 (MQ=255)

                                                                                                  ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                         >  6:165277/1‑139 (MQ=255)

                                                                                                  ggTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                         >  3:218607/1‑139 (MQ=255)

                                                                                                   gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            >  1:100521/1‑55 (MQ=255)

                                                                                                      tcGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCTCTGAGATAGGTGCCTCACTGATGAAGCATTgg                                     >  3:192709/1‑139 (MQ=255)

                                                                                                      tcGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCCCTGATTAAGCATTgg                                     >  1:187794/1‑139 (MQ=255)

                                                                                                       ggcgGTATCATTGGCGCCGGGGGGCCGGTGGGTACCCGCGCCGGTATGTTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACTAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                    <  5:272738/138‑1 (MQ=255)

                                                                                                             aTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTACCTGt                              >  2:92038/1‑139 (MQ=255)

                                                                                                              tCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                              >  3:292576/1‑138 (MQ=255)

                                                                                                                  tGCCGCACTGCGGCCATCGGGAAAGCCCCCCCGTCGCGTAGTTACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         <  2:269632/139‑1 (MQ=255)

                                                                                                                      gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  4:180898/1‑139 (MQ=255)

                                                                                                                      gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  3:4648/1‑139 (MQ=255)

                                                                                                                      gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  8:149191/1‑139 (MQ=255)

                                                                                                                        ccggggcCCAGATGGAAAGCCCTCCCGTATCGTTGTTACCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  6:234796/136‑1 (MQ=255)

                                                                                                                        aCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   >  8:48927/1‑139 (MQ=255)

                                                                                                                          gggggCCAGATTGAAACCCCTCCCGTTTCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  4:207023/138‑1 (MQ=255)

                                                                                                                          gggggCCAGATGTTAAGCCCACCCGTTTGTTATTAATCTCCACGACGGGGAGTCAGGCAACTATGTATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  3:350399/138‑1 (MQ=255)

                                                                                                                          gggggCCAGATGGAAAGCCCCCCCGTATGGTTGTTCGCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  5:370944/138‑1 (MQ=255)

                                                                                                                          gggggCAAGTGGGTACGCCCGCCCGTCGCTTTGTAAACTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  4:300980/138‑1 (MQ=255)

                                                                                                                           ggggCAGGATGGTAACCCCTCCTGTAGCTGAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  8:380309/138‑1 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGCACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGTCAGACCAAGTTTACt               >  6:31324/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  8:27026/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  3:258920/1‑139 (MQ=255)

                                                                                                                            gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACAACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    >  1:212915/1‑102 (MQ=255)

                                                                                                                               ccAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCa             >  6:197449/1‑138 (MQ=255)

                                                                                                                                   aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  3:168822/1‑138 (MQ=255)

                                                                                                                                   aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  1:48311/1‑138 (MQ=255)

                                                                                                                                        aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  2:10767/1‑138 (MQ=255)

AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  pSIJ663_pHT315_araE_xy/4488‑4763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`