BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,649	A→C	8.2%	Y260S (TAC→TCC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,649	0	A	C	8.2%	6450.2 / 139.2	2574	Y260S (TAC→TCC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base A (1665/643); new base C (9/252); total (1679/895)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2634 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                         >  7:86371/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCCGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGGTATCGACACGCCggg                                                                                                                                   >  4:382559/1‑137 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                   >  1:215711/1‑137 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGGTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GAGGGTAAGCCCTCCCGTATCGTAGTTATCTACacgacgtg                                                                                                                                   >  7:153127/1‑135 (MQ=255)

           cTTCTGCGCTCGCCCCTTCCGGCTCGCTGGTTTATTGCTGATAAATCTGGAGCCTGAGAGCGTCGGTCGCGCGGTATCATAGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCAGTATCGTAGTTATCTACACGACggg                                                                                                                                   >  5:364936/1‑137 (MQ=255)

             tCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGGGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGGATCGTAGTTAGCTACACGACGGGGAgg                                                                                                                               >  2:152261/1‑138 (MQ=255)

                 cgcgcgCCCCTTCCGGCTGGCTGGTTTATTGCTCAAAAATCGGGCGCGGGGGCGCGGGGGGCGCGGGGTATCATTCCCCCCGGGGGGCCA‑TATGGTACGCCCTCCCGTATCGTATTTATCAACACGACGGGGAGTCAgg                                                                                                                           <  2:62887/139‑1 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTTATAAATCTGGAGCCGGTGAGCGTGGGTCTGGCGGGTTCATTGCAGCACTGGGGCCA‑GCTGGTAAGCCCTCCCGTATCGTAGTTATATACACGACGGGGAGTCAGGCAACt                                                                                                                      >  2:294653/1‑139 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGctgtc                                                                                                                      >  1:382222/1‑135 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  6:196826/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  3:89386/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  4:142808/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  2:375521/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  2:324134/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGCTTTATTGCTGATAAATCTGGAGACGGTGAGATTGGGTCTCGATGTATCATTGAAGCACTGGGGCCA‑GATGGTAAGACCTCCCGTATCGTAGTTATCTACACGACGGTGAGTCAGGCAACt                                                                                                                      >  2:229881/1‑139 (MQ=255)

                        cccTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTAt                                                                                                                    >  7:52989/1‑139 (MQ=255)

                            tCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAt                                                                                                                >  4:351731/1‑139 (MQ=255)

                              ggggtggttgtTTTTTTGATGATAATAGTGGTGCGGGGGCGGGTGGGTCGGGGGTTATAATTGCACCAGTGGGGCAA‑GATGTAAAGCCCCCCGGTTTTGTTTTTTTATCCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                              <  8:163685/135‑1 (MQ=255)

                              cggcggGCTGGTTATTTGCTGATAAATCTGGCGCCGGTGCGGGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTTGTTATATCCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                              <  7:36692/139‑1 (MQ=255)

                              cGGCTGGCGTGTTTATGGCTGATACCGCGGGCGCCGGGTAGCGGGGGGCGCCCGGTATCATTCCCCCCCGGGGCCAA‑GCGGGAACGCCCTCCCTAATCGAAGTTACCAACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                              <  8:308967/139‑1 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGCGGAGTCAGGCAACTATGGATGAAc                                                                                                            >  4:185927/1‑139 (MQ=255)

                                   ggctgtTTTCTTGCTTATAAATCGGGCGCGGGGGAGGGTGGGGCTCGCGGTATCATTGCCGCCCTGGCGCCA‑GTTGGTACGCCCCCCCGTATCGTAGTTATCCACACGACGGGGAGTCAGGCAACTATGGATGAACGaa                                                                                                         <  4:45604/139‑1 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGACCGaa                                                                                                         >  2:123027/1‑138 (MQ=255)

                                        gTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGGTATCTACACGACGGGGAGTCAGGCAACTAGGGATGCACGAAATAGa                                                                                                    >  6:44886/1‑139 (MQ=255)

                                               gCTTATAAATCTGGCGCCGGTGTGGGGGGGGCTCGCGTTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATc                                                                                              <  8:250510/138‑1 (MQ=255)

                                               gCTGATAAATCTGGCGCGGGGGGGCGTGGGGCTCGCGGTATCATTGCAGCAGGGGGGCCA‑GTTGGTAAGCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATc                                                                                              <  7:299838/138‑1 (MQ=255)

                                                 gtaTAACTCGGGCGCCGGTGTGCGGGGGTCGCGCGGTATCATTCCAGCACTGGGGCCA‑GATGTTAAGCCCTCCCGAATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           <  6:27675/137‑1 (MQ=255)

                                                                gggggAGCGTGGGGCTCGCGGTACCATGGCCCCAGGGGGGCCA‑GTTGGTAAGCCCGCCCGTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  1:18281/135‑1 (MQ=255)

                                                                gggggAGCGGGGGTGGGGCGGCATCATTGCTGCTCTGGGCCCT‑TATGGTAAGCCCTCCCGTATCTTATTAATATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  8:232953/135‑1 (MQ=255)

                                                                gggTGCGGGTGGGTCTCGCGGAATAAGTGCCGCACGGGGGCCA‑TATGGCACCCCCTCCCGTATCTTCGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  4:89919/138‑1 (MQ=255)

                                                                gggTGCGGGTGGGGCGCGGGGTATCATGCCAGCACGGGGCCCA‑GTTGGAACCCCCTCCCTTATCGTATTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  5:126233/138‑1 (MQ=255)

                                                                cGGTGGGGGGGGGTCGCGTGGTATCAGTGCAGCACTGGGGCAA‑GATGGTACCCCATCCCGTATGGTATTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  7:204572/139‑1 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                          >  7:10621/1‑139 (MQ=255)

                                                                      ggtggggTCTGGCGTAATGATTGCAGCAGGGGCGCCA‑TTGGGAACGCCCCCCGGTTTGTTAGTATTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                      <  8:385935/135‑1 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCACCTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCCCTGATTAAg                                                                   >  7:179516/1‑139 (MQ=255)

                                                                         tGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAg                                                                   >  4:230762/1‑139 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  5:289592/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  7:256582/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  3:292576/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  2:209698/1‑138 (MQ=255)

                                                                                       tCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  1:123976/1‑138 (MQ=255)

                                                                                           tGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATCAAGCATTGGTAACTGTCAGAc                                                 >  2:262390/1‑139 (MQ=255)

                                                                                            gcagccCGGGGGCCA‑GATGGTAAGCCCTCCCGTAGCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 <  1:278817/138‑1 (MQ=255)

                                                                                             cagcaCTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACTAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                >  7:123828/1‑138 (MQ=255)

                                                                                                ccggggggCCA‑GAGGGTAGGCCCGCCCTTATGGTAGTATTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCGCACTGATTAAGCATTGGTAACTGTCAGACCAAGt                                            <  7:69562/135‑1 (MQ=255)

                                                                                                 ccTGGGGCCT‑GTTGGTAAGCCCTCCCTAATCTTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  8:338456/138‑1 (MQ=255)

                                                                                                 aCTTGGGCCA‑TTTGTTACGCCCTGCAGTTTTTTATTTATTTACACGACGGGGAGTCAGGCAACTATTGATGAACGAAATAGACAGATCGCTGAGTTAGGTGCCTCACTGATTAAGCATTGGTAACTGTAATACCAAGtt                                           <  8:277762/139‑1 (MQ=255)

                                                                                                  cgggggCCACGTTGGCACGCCCCCCCGTCTCGTCTTAACCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  1:304880/137‑1 (MQ=255)

                                                                                                   tGGGGCCA‑GTTGGAACGCCCTCCGCTATGTTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  2:88426/139‑1 (MQ=255)

                                                                                                   tGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         >  8:3659/1‑139 (MQ=255)

                                                                                                   tGGGCCCT‑GATTGTACCCCCTCCCTTCTCTTAGTTACATACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  1:44166/139‑1 (MQ=255)

                                                                                                   tGGGCCCA‑CTTGGCAACCCCTCCCGTAGCTAAGTAACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  3:128336/139‑1 (MQ=255)

                                                                                                   gggggCCT‑GAGGTTAAGCCCGCCCGTTTCTTAGTTATGTCCACGACGGGGAGTCAGTCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  8:71543/138‑1 (MQ=255)

                                                                                                   gggggCCA‑GATGTTAAGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  6:108234/138‑1 (MQ=255)

                                                                                                   gggggCCA‑GATGGTAAGCCCTCCCGTCTCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  1:64142/138‑1 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGCACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGTCAGACCAAGTTTACt                                       >  6:31324/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  7:39653/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  3:158076/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  3:322314/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  5:159933/1‑139 (MQ=255)

                                                                                                     ggcccA‑TATGGAAAGCCCACCCGTATCGTAGTTATAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       <  3:392366/136‑1 (MQ=255)

                                                                                                      gcccA‑TATGGCACGCCCTCCCGTCTGGTTGTAATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc                                      <  8:19548/137‑1 (MQ=255)

                                                                                                       gCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:34240/135‑1 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                   >  1:100941/1‑138 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  1:24889/139‑1 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                   >  6:141214/1‑138 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTAAGGATGAACGAAATAGACAGATCGCTCAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                   >  3:195561/1‑138 (MQ=255)

                                                                                                           ataTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  5:204267/137‑1 (MQ=255)

                                                                                                            gATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCGGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 >  3:57466/1‑139 (MQ=255)

                                                                                                             tgggTACGCCCGCCCGTAGCATAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGGGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  6:78739/135‑1 (MQ=255)

                                                                                                                 tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                            >  8:128132/1‑139 (MQ=255)

                                                                                                                  aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                            >  8:108994/1‑138 (MQ=255)

                                                                                                                      ccTCCCGTAGCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgat                       <  8:50246/139‑1 (MQ=255)

                                                                                                                       cTCCCGTACCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgatt                      >  4:269008/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  3:265583/83‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAt                                                                       <  6:351796/88‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:318307/118‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                    >  1:87434/1‑139 (MQ=255)

                                                                                                                          ccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         >  5:231956/1‑117 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa               >  1:340997/1‑138 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                <  7:234733/102‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 >  2:42818/1‑101 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         >  4:203772/1‑109 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  5:188711/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  6:382268/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  3:179405/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  6:222299/1‑139 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACAAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  4:164247/1‑139 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:75202/107‑1 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:337265/107‑1 (MQ=255)

                                                                                                                                      tATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  4:319048/70‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGCCAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  4:297290/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                      >  3:21321/1‑59 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                      >  1:146182/1‑75 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTa                                                                     <  4:113573/76‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           >  3:359097/1‑102 (MQ=255)

                                                                                                                                        tCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAttt     >  5:39909/1‑139 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTaa                                                                    <  5:251054/75‑1 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 <  6:168622/94‑1 (MQ=255)

                                                                                                                                          tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         >  7:275676/1‑101 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`