| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | pSIJ663_pHT315_araE_xy | 5,205 | G→A | 100% | intergenic (+474/–) | ampR → / – | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | pSIJ663_pHT315_araE_xy | 5,205 | 0 | G | A | 99.0% | 16441.3 / 3.7 | 5509 | intergenic (+474/–) | ampR/– | –/– |
| Reads supporting (aligned to +/- strand): ref base G (28/24); major base A (2550/2738); minor base C (135/23); total (2718/2791) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.72e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 5517 total aligned reads displayed.
GTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT > pSIJ663_pHT315_araE_xy/5067‑5340 | gCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCGTACCGGGTTGGACTCAAGACTATAGTTACCGGAGAAGGcacaccc > 1:248444/1‑136 (MQ=255) ccaccaCTTCAAGAACTCTGTAGCACCGCCTACATACCTCGATATGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCAAAGCgg < 7:319970/139‑1 (MQ=255) cAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTg > 5:186643/1‑138 (MQ=255) gAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACg < 6:285801/139‑1 (MQ=255) gAACTCTGTAGCACCGCCTACATACCTCGCGCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACg < 4:175952/139‑1 (MQ=255) ctGTAGCCCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAGCGGGTTGGACTAAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGCACgggggg > 4:325552/1‑139 (MQ=255) ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCGGGGTGGACTCAAGACGATATTTACCGGATAAGGCACAGCGGTCGGGCTGGACgggggg > 1:375709/1‑139 (MQ=255) ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTTCCGGATAAGGCACAGCGGTCGGGCTGAACgggggg > 2:262473/1‑139 (MQ=255) ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg > 5:352894/1‑139 (MQ=255) ccGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGca < 7:45882/139‑1 (MQ=255) ccGCCTACATACCTCGCGCTGCTAATCCTGGTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACGCAAGACGCTAGTTACCGGAGAAGGCACCGCGGGCGGGCTGCACGGGGGGTTCGTGcc > 8:18660/1‑138 (MQ=255) gCCTACATACCTCGCTCTGCTACTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca < 3:227194/139‑1 (MQ=255) gCCTACATACCTCGCTCTGCTAATCCTGTTCCGTGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGTCTGAACGGGGGGTTCGTGcaca < 8:328097/139‑1 (MQ=255) gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca < 1:65834/139‑1 (MQ=255) gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca < 5:239099/139‑1 (MQ=255) gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca < 2:53486/139‑1 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGTCTCAAGCCGATAGTTACCCGATAAGGCACAGCGGTCGGGCTGACCGGGGGGTTCGGGcgca > 5:112685/1‑138 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGCCTCAAGACGATAGTTTCCGGCTAAGGCACCGCGGTCGGGCGGAACGGGGGGTTCGTGcacac > 4:393285/1‑139 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCggg < 2:387159/116‑1 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac > 2:31715/1‑139 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGCGGTTGACTCAAGACCATAGTTACCTGATAAGGCAAAGCGGTCGGGCTGAACGGGGGGATCGTGcaca > 6:402541/1‑138 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTCACCGGGGTGGACTCAAGCCGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGcccac > 1:155596/1‑139 (MQ=255) ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAGACGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGcccac > 2:28968/1‑139 (MQ=255) ccTACATACCTCGCTCTGCAAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTAGTGTGTTACCGGGTTGGACACAAGACGAAAGTTAACGGATAAGGCCCAGCGGTCGGGCTGAACCGGGGGATCGTGcacac > 3:287912/1‑139 (MQ=255) cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCGGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc < 3:198379/139‑1 (MQ=255) ccTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCCAGCCGATAGTTTCCGGCTAAGGCACAGCGGTCGGGCTGTACGGGGGGGTCGTGCACCCCGCCCacc > 6:222464/1‑137 (MQ=255) cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCt > 5:331830/1‑139 (MQ=255) cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCGTACCGGGGTGGACTCAAGGCGCTAGTTACCGGAGAAGGCACCGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCCGcg > 3:262790/1‑138 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGGCTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGACCGGGGGGTTCGTGCACACAGCCCAGCTTg > 1:100323/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCGTg > 7:258980/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGGTGGGCTCAACACGATACTTAACCGATAAGGACCACCGGTCCGGCTGAACGGGGGGTTCGTGCCCACCGCCCAgggtg > 2:189979/1‑135 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCCGCTTg > 7:21726/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc > 5:383386/1‑131 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg > 5:160480/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg > 8:300583/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg > 3:71618/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg > 3:112536/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAAAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCAGCTTg > 5:84181/1‑139 (MQ=255) cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGGCTCAAGACGATAGTTACCGGATAAGGCCCAGCGGGCGGGCTGCCCGGGGGGTTCGTGCACACCGCCCAGCtag > 2:123817/1‑137 (MQ=255) cGCTCTGCTAATCCTGATACCAGTGGCTGCTGACAGTGGCGATAAGTCGTGTCTTACAGGGTTGGACTCAAGACGATAGTTACCTGATAACGCACAGCGGAAGGGCTGAAAGTGGGGTTCGTGCACACAGCCCACCTTg > 8:246027/1‑139 (MQ=255) cGCTCTGCTAATCCAGTTACCAGTGGCTGCTCCCAGTGGCGATAAGTCGTGACTTACCGGGTTGGACTCAAGACGAAAGGTACCGGATAAGGCACAGCGGTCGGGCTGCACGGGGGGTTCCTGCACACAGCCCAGCATg > 8:47132/1‑139 (MQ=255) gCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGTAGATAATTCGTGTCTTACCTGGGTGGGCTCAAGCCTATAGTGACCGGGTTAGGCAAAGCCTTACTGCTGAACGGGGGGGTTGTTCACACAGCCCAGcgttg > 4:86853/1‑135 (MQ=255) gCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca > 4:298874/1‑126 (MQ=255) cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAATACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGc < 6:140746/136‑1 (MQ=255) cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa < 3:316486/139‑1 (MQ=255) cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa < 6:163724/139‑1 (MQ=255) cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa < 8:347994/138‑1 (MQ=255) ccTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAcc > 4:200986/1‑139 (MQ=255) gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGa > 3:41030/1‑126 (MQ=255) gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCTa > 1:221523/1‑138 (MQ=255) gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTa > 8:130600/1‑138 (MQ=255) tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGGCTCAAGACGATAGTTACCGGATAAGGGACAGCGGTCGGGCGGAACGGGGGGTTGGTGCACACCGCCCCGCGTGGGGCGCACGACCTacac > 2:88206/1‑139 (MQ=255) tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTacac > 3:173940/1‑139 (MQ=255) cAGTGGTTGCTGGCAGTGGCGATATGTCGTGTCTTACCTGGTTGGACTCAAGACGATAGTTACCGGATATGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACTCAGCCCAGGTTGGAGCGAACGACCTACTCCg < 2:346407/138‑1 (MQ=255) aGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa < 5:21168/139‑1 (MQ=255) tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACCCAGCCCAGCTTGGAGCGAACGACCTACACCGAACt > 7:376774/1‑139 (MQ=255) tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt < 7:104539/139‑1 (MQ=255) tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCGGAACGGGGGGGTCGTGCACACAGCCCAGCGTGGAGCGAACGACCTACACCGAACt < 7:241019/139‑1 (MQ=255) gggCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt < 7:135959/138‑1 (MQ=255) gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga < 4:151192/139‑1 (MQ=255) gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga < 3:348207/139‑1 (MQ=255) gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga < 4:56583/139‑1 (MQ=255) ctgctgCCAGTGGCGATAAGTCTTGTCTTACCGGGATGGACTCAAGACGAAAGTTACCGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgag < 7:293219/139‑1 (MQ=255) ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCgg < 8:161397/74‑1 (MQ=255) ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAAC‑GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga < 1:4396/139‑1 (MQ=255) tgctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga < 6:320361/139‑1 (MQ=255) tgctgcCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga < 5:268962/139‑1 (MQ=255) ggctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga < 8:266531/138‑1 (MQ=255) tctgcCAGTGGCGATAAGTCGTTTATTACCGGGTTGGAATCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaaa < 4:236633/137‑3 (MQ=255) gctgcCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGTCGATAGTTACCCGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCCGCTTGGAGCGCACGACCGACACCGAACAgaga > 1:126570/1‑138 (MQ=255) cAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa < 1:282667/139‑1 (MQ=255) gTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGca < 8:101801/90‑1 (MQ=255) ggCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGc > 6:379483/1‑139 (MQ=255) gCGATAAGTCGTGTCTTACCGGGGTCGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg < 8:381494/139‑1 (MQ=255) gATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg < 4:268586/139‑1 (MQ=255) gATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACAAATGCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg < 2:15390/139‑1 (MQ=255) tCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCGACACCGCACTGAGATACCTACAGCGTGAGCTAt > 4:124898/1‑139 (MQ=255) tgtCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga > 3:383194/1‑117 (MQ=255) tCTTACGGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTGGGGCTGAAGGGTGGGTTCGTGCACACTGCCCAGCTTGTAGCGAACTACCTAAACCGAACTTAGATACCTACAGCTTGATATATgata < 6:347748/138‑3 (MQ=255) tCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATgaga < 7:7094/138‑1 (MQ=255) tCTTACCGGGTTGGACGCAAGACGATAGTTACCGGATAAGGCACTGGGGTCGGGCTGAACGGGGTGTTCGTGCACACAGCCCAGGTTGGAGCGAACGACCTACACCTAACTGAGATACCTACAGGGTGAGCTATtaga < 2:225939/138‑1 (MQ=255) ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa < 5:46306/89‑1 (MQ=255) ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc > 7:3465/1‑91 (MQ=255) ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc > 3:145091/1‑139 (MQ=255) ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc < 5:304649/139‑1 (MQ=255) ggTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga > 1:95777/1‑107 (MQ=255) ttGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGc < 8:217009/139‑1 (MQ=255) aaGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg < 7:161921/48‑1 (MQ=255) aaGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGa < 8:67965/138‑1 (MQ=255) aaGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGa < 6:113014/138‑1 (MQ=255) gACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa > 1:18889/1‑101 (MQ=255) aTAGTTACCGGATATGGCACAGCGGTCGGGCTTAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCCAACGACCTACACCGAACTGATAAACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGgaga < 3:97208/139‑1 (MQ=255) aTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca < 6:253156/52‑1 (MQ=255) tAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg < 4:356611/41‑1 (MQ=255) aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCg > 5:134524/1‑139 (MQ=255) cGGTTAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCTGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa < 7:188699/139‑1 (MQ=255) cGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa < 6:73808/139‑1 (MQ=255) ggATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa < 1:34743/61‑1 (MQ=255) ggATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGAc < 1:312130/139‑1 (MQ=255) ggCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa < 6:195283/132‑1 (MQ=255) | GTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT > pSIJ663_pHT315_araE_xy/5067‑5340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |