BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,673	A→C	7.0%	intergenic (+1942/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,673	0	A	C	7.0%	9229.7 / 87.2	3336	intergenic (+1942/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (1640/1321); new base C (12/300); total (1654/1682)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3361 total aligned reads displayed.

GGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAG  >  pSIJ663_pHT315_araE_xy/6535‑6811

    ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTTTTGAGCTGCAGCGCTTAAAGCtt                                                                                                                                          >  1:181646/1‑138 (MQ=255)

    ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          <  1:281603/139‑1 (MQ=255)

    ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          >  2:83339/1‑139 (MQ=255)

    ccATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          <  5:104267/139‑1 (MQ=255)

    acATGGTGATGAGGCAAACCACCGAACACCCCCTTCGCCTGGGGAGTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          <  6:693/138‑1 (MQ=255)

       tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAACCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                       >  7:197195/1‑139 (MQ=255)

       tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTggg                                                                                                                                        >  4:103764/1‑138 (MQ=255)

       tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          <  5:219797/136‑1 (MQ=255)

       tGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                       >  8:13418/1‑139 (MQ=255)

         ttaatgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  8:293019/136‑1 (MQ=255)

         ttGATTAGGCAAACCACAGAAAACCCCCTTGGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACa                                                                                                                                     <  3:178252/139‑1 (MQ=255)

         ttGATGAGTCAAACCAAAGAACACCCCCTTCGCCTGGGGATTCTTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAACCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  6:130725/139‑1 (MQ=255)

         ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  5:174047/139‑1 (MQ=255)

         ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  3:200509/139‑1 (MQ=255)

         ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  3:162553/139‑1 (MQ=255)

         ttGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCACCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  7:222038/139‑1 (MQ=255)

         ttGATGACTCAAACCAAAGAAAACCCCCTTCGCCTGGTGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAATCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCCGCGATTAAAGCTGGGACa                                                                                                                                     <  3:13334/139‑1 (MQ=255)

         ttGAGGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGGGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  5:192965/139‑1 (MQ=255)

            atgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACCCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTg                                                                                                                                  >  1:236432/1‑139 (MQ=255)

             tgaGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGc                                                                                                                                 >  8:354266/1‑139 (MQ=255)

               aGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                <  3:406462/138‑1 (MQ=255)

               aGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                <  8:318930/138‑1 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGTGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                             >  7:307827/1‑139 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAg                                                                                                                                             >  2:140425/1‑123 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAATCTGGGACATTGCTCAg                                                                                                                             >  3:103552/1‑139 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTg                                                                                                                                          >  8:16923/1‑126 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                             >  3:25169/1‑139 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                             >  3:283944/1‑139 (MQ=255)

                 tCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                             >  7:390975/1‑139 (MQ=255)

                   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     <  4:219605/129‑1 (MQ=255)

                   aaaCCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAg                                                                                                                             >  6:239701/1‑137 (MQ=255)

                         aaGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGtt                                                                                                                      >  5:374042/1‑138 (MQ=255)

                         aaGAAAACCCCCTTCGCCTGGGGATTAGTGTGTGGCGTCCGCTCGCCGGAAAGATAAGGCGGAACCCAACCTGGCTCGGCCCTTTCATCCGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGtga                                                                                                                     >  6:400905/1‑137 (MQ=255)

                         aaGAAAACCACCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGTAAAGATAAGGCAGAACCCAAACTGGCTCGGAACTTTCATCAGCCTGTTGCGCTGCAGCGATTAACGCTGGGACATTTCTCAGTCCGGTTa                                                                                                                     >  6:376214/1‑139 (MQ=255)

                          aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTGa‑a                                                                                                                   >  7:72450/1‑139 (MQ=255)

                           gAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGc                                                                                                                                            >  4:400012/1‑114 (MQ=255)

                               ccccccTTCGCCTGGGGAGTATTGGGGGGCGTACGCTCGCCGGACAGATAAGGCAGAAACCAACCTGGCTCGGCACTTTCCTCAGCGGGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTG‑cg                                                                                                             <  1:127517/138‑1 (MQ=255)

                               aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAgg                                                                                                                            <  7:256735/126‑1 (MQ=255)

                               aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑cg                                                                                                             <  4:257406/139‑1 (MQ=255)

                                cccccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑cgc                                                                                                            >  7:338093/1‑139 (MQ=255)

                                 ccccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGACTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                <  8:233775/120‑1 (MQ=255)

                                   ccTTCGCCTGGGGATTTTTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAACCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGCCATTGCTCAGGCCGGTTA‑ATTT‑CGCGGc                                                                                                         >  2:248118/1‑139 (MQ=255)

                                    cTTCGCCTTGGGATTTTTGTGTGGCGTACGCGCGCCGTAAAGATAAGGCAGAAACCAAGCTGGCTCGGCACTTTCATCAGCCTGTTGAGCGGCAGCGATTAAAGCTGGGACATTGCTCAGGGCGGTTA‑TTTT‑CGCGGcc                                                                                                        <  7:150370/139‑1 (MQ=255)

                                    cTTCGCCTGGGGATTTTTGTGTGGGGTCCGCTCGCCGGAAAGATACGGCAGAAACCAAACTGGCTCGGCACTTTCATAAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGATCAGCCCGGTTA‑ATTT‑CGCGGcc                                                                                                        <  3:33111/139‑1 (MQ=255)

                                    cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGcc                                                                                                        <  5:127516/139‑1 (MQ=255)

                                    cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGcc                                                                                                        <  1:10121/139‑1 (MQ=255)

                                    cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGcc                                                                                                        >  6:274715/1‑139 (MQ=255)

                                    cTTCGCCTGGGGATTATCGGGGGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACGGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCGCAGGCCGGTAA‑ATTT‑CGCGGcc                                                                                                        <  2:374002/139‑1 (MQ=255)

                                        gCCTGGGGATTATTGGGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAAt                                                                                                    <  6:155723/139‑1 (MQ=255)

                                                  tattGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCt                                                                                                                                >  6:18063/1‑103 (MQ=255)

                                                    ttGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACacgacg                                                                                        >  1:159409/1‑139 (MQ=255)

                                                    ttGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACacgacg                                                                                        >  1:30870/1‑139 (MQ=255)

                                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  4:188746/1‑139 (MQ=255)

                                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  1:257472/1‑139 (MQ=255)

                                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  1:370401/1‑139 (MQ=255)

                                                         tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                   >  1:52524/1‑139 (MQ=255)

                                                         gggCGGACGCCCGCCGGAAAGATACGCCAGAAACCAACCTGGCGCGCCACTTTCATCAGCCTGTGGAGCGGCAGCGATTAAAGCGGGGACATTGCTCCGGCCGTTTA‑ATTC‑CGCGCCCCAATCCAGCCACGACGCTGCa                                                                                   <  6:103519/138‑1 (MQ=255)

                                                            cGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACt                                                                                 >  3:378201/1‑138 (MQ=255)

                                                             gTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACt                                                                                 >  5:404919/1‑137 (MQ=255)

                                                             gTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCa                                                                               >  3:226473/1‑139 (MQ=255)

                                                              tACGCTCGCCTGAAACATAAGCAAGAACCCATACTGGCGCGGAACTTTCTTCAGCCTTTTGTGCTGCTGCGATTAATGGGGGTAAATTGCTCAGGCGGGTTA‑TTTT‑CGCGGCCAAATCCAGACACGACGCTGCACTCAg                                                                              <  8:386252/139‑1 (MQ=255)

                                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                              >  5:272010/1‑139 (MQ=255)

                                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                              >  3:307718/1‑139 (MQ=255)

                                                              tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACGTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCGACTCCAGACACGACGCTGCACTCAg                                                                              >  2:218036/1‑139 (MQ=255)

                                                                 gCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGCCACGACGCTGCACTCAGCAt                                                                           >  2:185986/1‑139 (MQ=255)

                                                                     gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                         >  7:279202/1‑137 (MQ=255)

                                                                       cGGAAACATAAGCCACAAACCAAACGGGCGCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATAAACGCTGGGACATTGCTCAGGCCGGTTA‑ATTC‑CGCGGCCCAATCCACCCACGACGCTGCACTCAGCATCACAGa                                                                     <  5:346985/139‑1 (MQ=255)

                                                                               tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACa                                                                                                                                     >  3:355622/1‑69 (MQ=255)

                                                                               tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAAt                                                             >  5:153881/1‑139 (MQ=255)

                                                                                 aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                           >  4:78875/1‑139 (MQ=255)

                                                                                  gccataaaCAAAACTGGCTCGGCACTTTCTTCAGCCTGTTGAGCTGCAGCTATTAAAGAGGGGACATTGCTCAGGCCGTTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                          <  7:36501/134‑1 (MQ=255)

                                                                                    cAGAAACCACAGTGGCGCGGCAATTTCTGCTGCCTGTTGAGAGGCCGCTATTAACGTGGGTACTTGGCCAAGGCCGTATA‑ATTG‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                        <  8:73822/139‑1 (MQ=255)

                                                                                       aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCACTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                     >  2:283434/1‑139 (MQ=255)

                                                                                       aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                     >  5:71949/1‑139 (MQ=255)

                                                                                         aCCAACCGGGCGGGGCACTTTAATCCGCCTGTTGAGCGGCAGCGTTTAAAGCTGGGACATTGCTCAGGCGGGTTA‑TTTT‑CGCGCCCAAATCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTg                                                   <  8:109135/139‑1 (MQ=255)

                                                                                            aacgtGGCGCGGCATTTACATCTGCCTGTTGCGCTCCAGCTATAAACGGGGGGACATTCCTC‑GGGCGGTTATATTG‑CGCGGCCAAATCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                <  6:207516/135‑1 (MQ=255)

                                                                                             aaCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGccc                                                                                                       >  2:337129/1‑83 (MQ=255)

                                                                                             aaCTGGCTCGGCACTGTCATCAGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAGTCCAGACACGACGCTGCACTCCGCATCCCAGACATTAAATGCCAACGTTGCgg                                                >  6:348952/1‑138 (MQ=255)

                                                                                              aCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                >  5:325593/1‑137 (MQ=255)

                                                                                                   cTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCa                                         >  1:80835/1‑139 (MQ=255)

                                                                                                         aCTTTCATCCGCCTGTTGCGCTGCAGCTATAAAAGCGGGGACATTCCTCCGGCGGTTTA‑ATTG‑CCCGGCCCAACCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                   <  8:201042/139‑1 (MQ=255)

                                                                                                         aCTTACATCCGCCGGTTCCGCTGCCGCTATAAACGCGGGGACATTGCTCAGGCCGGTAA‑ATTC‑CGCGGCCCAATCCCCCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                   <  5:302991/139‑1 (MQ=255)

                                                                                                            ttCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGa                                                                                                                                       <  5:245143/38‑1 (MQ=255)

                                                                                                             tcatcaGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGc                               >  8:261508/1‑139 (MQ=255)

                                                                                                               atccGCCTGGTGAGCTGCAGCTATTAACGCGGGCACATGGCCCCGGCGGTTTA‑ATTT‑CGCGCCCCAAACCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCt                              <  7:170803/138‑1 (MQ=255)

                                                                                                                  aGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACATACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATg                           >  6:96541/1‑138 (MQ=255)

                                                                                                                    ccTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAc                        >  3:74242/1‑139 (MQ=255)

                                                                                                                     cTGTTCCGCGGCCGCGATAAACGCGGGGACATGGCGCAGGCCGGTTA‑ATTC‑CGCGCCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACg                       <  6:308730/139‑1 (MQ=255)

                                                                                                                        ttGAGCGGCCGCGATTAACGCTGGGACATTGCTCAGGCCGGTAA‑ATTT‑CGCGGCCCAATCCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                      <  1:21060/137‑1 (MQ=255)

                                                                                                                             cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTc               >  1:99742/1‑139 (MQ=255)

                                                                                                                                cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGAGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg            >  1:345246/1‑139 (MQ=255)

                                                                                                                                 cgCGATTAAAGGTGGGAATTTGCTCAGGCGGGTTA‑TTTT‑CGCGGCCCAATCCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc           <  7:121101/138‑1 (MQ=255)

                                                                                                                                 aGCGATTAAAGCGGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc           <  2:55344/139‑1 (MQ=255)

                                                                                                                                 aGCGATTAAAGCGGGCACATTGCTCAGGCCGGTAA‑ATTG‑CGCGGCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc           <  4:140652/139‑1 (MQ=255)

                                                                                                                                  gcttttAACGCGGGGACATTGCTCTGGCCGGTAA‑ATTC‑CGCGGCCAAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  4:297003/135‑1 (MQ=255)

                                                                                                                                  gCGTTTAAAGCTGGGACATTGCTCAGGCGGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  8:50703/139‑1 (MQ=255)

                                                                                                                                  gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  4:167374/139‑1 (MQ=255)

                                                                                                                                   ctaTTAAAGCGGGTCAATTGCTCAGGCC‑GTTA‑ATTTGCGCGCCCCAATCCCCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct          <  4:3313/136‑1 (MQ=255)

                                                                                                                                     aTTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGCCGCTGCACTCCGCATCACAGACATTAAATGCCAACGTTGCGGTAGAGCATGGC‑AAAGCTATGTACGGCGCTTGCTGgcttgcg      >  7:53475/1‑138 (MQ=255)

                                                                                                                                         aaGCGGGTACATTGCTCAGGCGGTTTA‑ATTT‑CGCGCCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTaa   <  4:366131/139‑1 (MQ=255)

GGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTA‑ATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAG  >  pSIJ663_pHT315_araE_xy/6535‑6811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`