BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,718	A→C	9.1%	intergenic (+1987/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,718	0	A	C	9.1%	5551.1 / 148.9	2667	intergenic (+1987/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base A (1506/867); new base C (44/250); total (1550/1117)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2835 total aligned reads displayed.

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGAT  >  pSIJ663_pHT315_araE_xy/6580‑6856

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGa                                                                                                                                            >  6:100221/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGa                                                                                                                                            >  3:329709/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGa                                                                                                                                            >  8:129257/1‑139 (MQ=255)

gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGAATAAAGCTGGGACATAGCACAGGCCGGTTAATTT‑CGCGGCCCAAA‑CCAGa                                                                                                                                            >  4:283546/1‑139 (MQ=255)

  tattattGTGGGGCGTACGCGCGCCGGAAATATAAGGCAGAAACAAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGaca                                                                                                                                          <  1:16003/138‑1 (MQ=255)

     tattGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACacga                                                                                                                                       >  1:220717/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGGTGCa                                                                                                                                >  7:389818/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCa                                                                                                                                >  1:39520/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCa                                                                                                                                >  1:58786/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCa                                                                                                                                >  6:38544/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCa                                                                                                                                >  2:348238/1‑139 (MQ=255)

            tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCa                                                                                                                                >  8:267928/1‑139 (MQ=255)

                gTACGCTCGCGGTAAAGATACGGCAGAAACAAACCTGGCTGGGCACTTGAATCCGCCTGTTGAGCTGCAGCTATTAACGCGGGGACATTGCTCAGGCCGGTTAATTC‑CGCGGCCAAAT‑CCAGACACGACGCTGCACTCa                                                                                                                            <  8:300795/139‑1 (MQ=255)

                gTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCa                                                                                                                            >  3:226473/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAg                                                                                                                           >  3:354875/1‑139 (MQ=255)

                 tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATAAGCCTGTAGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCAcctgt                                                                                                                           >  3:12503/1‑135 (MQ=255)

                        gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATca                                                                                                                      >  1:261233/1‑137 (MQ=255)

                                  tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGCCactgtct                                                                                                          >  1:283408/1‑133 (MQ=255)

                                  tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAAt                                                                                                          >  2:253651/1‑139 (MQ=255)

                                    aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                                                                        >  5:351299/1‑139 (MQ=255)

                                     gcaataaaCCAAACTGGCGCGGCCATTTGATCAGCCTGTTGAGCTGCAGGGATAAATGCGGGGACATGGCTCAGGCCGGTAAATTT‑CGCGGCCCAAG‑CCAGACAAGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                                       <  7:263975/134‑1 (MQ=255)

                                       cacaaaCCAACCGGGCGCGGCACTTTCATCAGCCTGGTTCGCGGCAGCTATTAAAGGTGGGAAATTGCTCAGGCCGGTAAATTG‑CGCGGCCCAAT‑CCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                                                                     <  1:241062/136‑1 (MQ=255)

                                          aacccAAACTGGCTCGGCACTTTCAGCAGCGTGTTGAGCTGCAGCTATTAATGCTGGTACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                                                  <  6:356681/136‑1 (MQ=255)

                                          aaaCCAAAGTGGCGCGGCACTTTCACCCGCCTGTTGAGCTGCAGCGATAAAAGCTGGGACATTGCTCAGGCCGGTTAATTG‑CGCGGCACAAT‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                                                  <  5:328595/139‑1 (MQ=255)

                                                 aCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCgg                                                                                             >  6:49669/1‑137 (MQ=255)

                                                    ggCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcg                                                                                        >  5:370452/1‑139 (MQ=255)

                                                     tcgcgtcACTGTAATCAGCCTGTTTCGGTGATGCGTTAACTGCTGGGACTTTGCTCCGGCGGGTTATTTT‑CGCGGCCAAAT‑CCACCCACGACGCTTCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  8:392743/133‑1 (MQ=255)

                                                     tcTCGGCACTTTCATCCGCCTGTTGAGCTCCAGCGATTAAAGCTGGCACTTTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  8:362312/138‑1 (MQ=255)

                                                     gCTCGGCACTTTCATCCGCGGGTTGCGCTGCAGGTATTAAAGGTGGGAAATTGCGGTGGCGGTTAAATTT‑CGCGCCCCAAT‑CCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  7:56968/139‑1 (MQ=255)

                                                     gCTCGGCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAACGCGGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  7:28104/139‑1 (MQ=255)

                                                     gCTCGGCACTTTCATCAGCCTGTTGAGCTGACGCGATTAACGCGGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                                       <  8:61882/139‑1 (MQ=255)

                                                            attttCTTCTGCCTGTTTAGGTGCAGCTATTAATGGTGTGCCATTTCGCTGGCGGGTTATTTT‑GGGGGCCAAAT‑CCTCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  4:385945/137‑1 (MQ=255)

                                                               agacataGCCTTTTGTGCTCCTGCTATTAAAGAGGGGACATTGCTCAGGCGGGTTATTTT‑CGCGGCCAAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                                             <  8:214141/133‑1 (MQ=255)

                                                                tcatcaGCCTGTTCAGCGGCAGCGATTAACGCTGGGAAATGGCTCCGGCCGTTTATTTT‑CGCGGCCCAAC‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                                             <  4:119452/138‑1 (MQ=255)

                                                                         tGTTGCGCTGCTGCTTTTAATGGTGGTAAATTGCTCTGGCGTTTTATTTT‑GGGGGCCAAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                                                                   <  4:17781/139‑1 (MQ=255)

                                                                           ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgcg                                                                 >  5:397161/1‑139 (MQ=255)

                                                                           ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgcg                                                                 >  6:294850/1‑139 (MQ=255)

                                                                            tGAGCTGCAGCGATTAAAGCTGTGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTTAGGTAGAGAATGGCAAAAGCTATGTAcctgt                                                                 >  7:131852/1‑134 (MQ=255)

                                                                                cTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCtttg                                                            >  1:311491/1‑138 (MQ=255)

                                                                                 tGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCCCTCAGCAGCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTAGGTACGGCGCTTGCt                                                           >  7:158993/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  2:62995/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGAGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                                         >  1:345246/1‑139 (MQ=255)

                                                                                   cAGCGATTAAAGATTTGACATTGATCAGGCCGGTTAATTT‑CGCGGCACAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCCCATGCCAAAAGCAATCTACTTCGCTTTCTgg                                                         >  7:217449/1‑139 (MQ=255)

                                                                                    aGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                                                        <  8:77081/139‑1 (MQ=255)

                                                                                    aGCGATTAAAGCGGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                                                        <  3:159688/139‑1 (MQ=255)

                                                                                     gctaTTAAAGCGGGGACTTTGCTCTGGCCGGTTAATTT‑CGCGGCCCAAT‑CCACCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  2:100598/136‑1 (MQ=255)

                                                                                     gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       >  8:157876/1‑139 (MQ=255)

                                                                                     gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGATATGTCCGGCGCTTTCTGgca                                                       >  2:390976/1‑138 (MQ=255)

                                                                                     cctaTTAACGCGGGTACATTGCTCCGGCGGTTTAATTG‑CGCGGCCCAAC‑CCCCACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  3:283336/136‑1 (MQ=255)

                                                                                     ccatttATAGCGGGGACTTTGACCAGGCGGGTAATTTT‑CCCGGCCACAC‑CCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCTACGTTTCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  8:327259/135‑1 (MQ=255)

                                                                                      cGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgctt                                                      <  5:172126/139‑1 (MQ=255)

                                                                                        aTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                                                    >  7:31455/1‑139 (MQ=255)

                                                                                        aTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGAATCGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                                                    >  2:92469/1‑139 (MQ=255)

                                                                                            acgcggggACTTTGGTCCGGCCGTTTAATTT‑CGCGGCCCAAT‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTaa                                                <  4:309981/134‑1 (MQ=255)

                                                                                              gcggggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGa                                              <  3:377863/136‑1 (MQ=255)

                                                                                              gcggggACATGGCGCCGGCCGTTAAATTT‑CGCGGCCCAAT‑CCATACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGa                                              <  5:13031/136‑1 (MQ=255)

                                                                                              gCTGGGACATTGCTCGGGCCGGTAAATTG‑CGCGCCCAAAT‑CCCTCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGa                                              <  6:84398/139‑1 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAcccc                                          >  5:250263/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                          >  3:252231/1‑139 (MQ=255)

                                                                                                  ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                                          >  1:192831/1‑139 (MQ=255)

                                                                                                       ttGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGc                                     >  5:152366/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAcccctgtct                                    >  3:306270/1‑133 (MQ=255)

                                                                                                        tGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  1:78705/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  7:356213/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  8:184962/1‑139 (MQ=255)

                                                                                                        tGCTCAGGCCGGTTAAATT‑CGCGTCACAAT‑CCCGACACGACGCTGCACTCAGCATCACAGACAATAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTAAAGAACCCTACGCt                                    >  6:166710/1‑139 (MQ=255)

                                                                                                         ggcccggCCGTTTATTTT‑CGCGGCACAAA‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGTCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    <  4:241828/133‑1 (MQ=255)

                                                                                                          cTCCGGCCGTTAAATTG‑CGCGGCCCAAT‑CCCCCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                  <  6:331865/139‑1 (MQ=255)

                                                                                                          cTCAGGCCGGTTAATTT‑CGCGGCCAAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGa                                  <  3:67953/139‑1 (MQ=255)

                                                                                                             cggCCGGTAAATTG‑CGCGGCCCAAG‑CCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  7:7514/138‑1 (MQ=255)

                                                                                                             cgcccGGTTAATTG‑CGCGGCCCAAA‑CCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  7:115036/136‑1 (MQ=255)

                                                                                                             aGGCGGGTAAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  2:228603/139‑1 (MQ=255)

                                                                                                             aGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  1:367138/90‑1 (MQ=255)

                                                                                                             aGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               >  2:146051/1‑139 (MQ=255)

                                                                                                               gCCTGTTATTTT‑CGGGGCCCTAA‑CCTTACACGACGCTGCACTCAGCATCACAGACATTAAATGCAAAGGTTGCGGTAGCGCATGGGAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCa                             <  6:140037/139‑1 (MQ=255)

                                                                                                               gCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGTCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc                              >  3:199040/1‑138 (MQ=255)

                                                                                                               gCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGACCCCTTCGCTGCCAGc                              >  2:204870/1‑138 (MQ=255)

                                                                                                               gCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACCGc                              >  7:242820/1‑138 (MQ=255)

                                                                                                                gcGGTTTATTTGCGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg                               <  4:354168/136‑1 (MQ=255)

                                                                                                                  ggTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    <  5:66007/129‑1 (MQ=255)

                                                                                                                  ggTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  5:189846/1‑139 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                                                     <  6:246967/110‑1 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                                       <  4:202180/108‑1 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    <  3:92895/127‑1 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  3:179739/1‑137 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  5:305010/1‑137 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  4:59162/1‑137 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  5:278296/1‑137 (MQ=255)

                                                                                                                    ttAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa                          >  6:184447/1‑137 (MQ=255)

                                                                                                                        ttt‑CCCCGCCCAAACCCAGACACGACGCTGCACTCTGCATCACAGACATTAAATGCCAACGTGGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGAc                     <  8:104750/139‑1 (MQ=255)

                                                                                                                         tt‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgc                                                        >  5:217554/1‑102 (MQ=255)

                                                                                                                              cgGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACg                                                                                                   >  1:100164/1‑55 (MQ=255)

                                                                                                                              cgGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                                                <  4:95452/74‑1 (MQ=255)

                                                                                                                              cgGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGCCCCCCGa               >  1:131002/1‑139 (MQ=255)

                                                                                                                              cgGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGa               >  2:295342/1‑139 (MQ=255)

                                                                                                                                     aT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTcccc        <  8:23948/139‑1 (MQ=255)

                                                                                                                                          aGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCg    <  7:186641/139‑1 (MQ=255)

                                                                                                                                            acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACg                                                                    <  6:20908/73‑1 (MQ=255)

                                                                                                                                            acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt                                    >  3:401819/1‑105 (MQ=255)

                                                                                                                                            acacGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGa   >  4:151416/1‑138 (MQ=255)

GGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAAT‑CCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATAGACCCCCGACGTCCCCAGCGAT  >  pSIJ663_pHT315_araE_xy/6580‑6856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`