BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	8,624	T→G	5.3%	intergenic (+3893/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	8,624	0	T	G	5.3%	12251.1 / 162.8	4865	intergenic (+3893/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (2235/2271); new base G (326/26); total (2564/2301)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5157 total aligned reads displayed.

TCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAAC  >  pSIJ663_pHT315_araE_xy/8486‑8762

 ccACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGGTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                            <  7:228946/138‑1 (MQ=255)

       tGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCtt                                                                                                                                      >  3:13689/1‑138 (MQ=255)

       tGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGCAAAGAGTCACCTGCCAGCGTCGCGTGGTTAGCtt                                                                                                                                      >  1:59736/1‑138 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCTTCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAgggtgaa                                                                                                                                   >  1:290670/1‑133 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAg                                                                                                                                         >  6:372790/1‑133 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCaa                                                                                                                                   >  2:255737/1‑139 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                    >  4:108429/1‑138 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                    <  3:108429/138‑1 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                    <  5:332628/138‑1 (MQ=255)

         ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGGCGCGTGGGTAGGTTcca                                                                                                                                   >  5:303163/1‑137 (MQ=255)

         ttCGGGAACTGGTCGGGGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCa                                                                                                                                    <  8:187219/138‑1 (MQ=255)

             ggAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               <  6:91389/139‑1 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGACCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGttt                                                                                                                         >  8:209592/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGACCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCGTCAATGTTCAGttt                                                                                                                         >  8:262804/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGACCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTGAGCGTCAATGTTCAggt                                                                                                                          >  5:123787/1‑136 (MQ=39)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGt                                                                                                                                            <  8:353092/120‑1 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCTTCAATGTTCAGttt                                                                                                                         >  4:23431/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               <  3:222993/133‑1 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               <  5:38288/133‑1 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               >  7:204340/1‑133 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGttt                                                                                                                         >  4:348364/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                          >  4:93745/1‑138 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGtt                                                                                                                          >  6:101016/1‑138 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTAAtttt                                                                                                                         >  1:349493/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTAGTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCGTCAATGTTGAGttt                                                                                                                         >  3:344246/1‑139 (MQ=255)

                   ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGATGGCGTCGACAGAACCGAACACGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCTCGTGGTTTGCTTCAATCTTCAGtg                                                                                                                          >  7:40386/1‑137 (MQ=39)

                     tCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                       <  2:192327/139‑1 (MQ=255)

                     tCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                       <  2:144166/139‑1 (MQ=255)

                     tCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                       <  1:390868/139‑1 (MQ=255)

                         tgtCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAAtctc                                                                                                                   <  4:298510/139‑1 (MQ=255)

                         tgtCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAAtctc                                                                                                                   >  4:396900/1‑139 (MQ=255)

                          gtCCCAGCCCAGTTGCGCATCGCCACGGGTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATctct                                                                                                                  <  1:232198/139‑1 (MQ=255)

                            cccagcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCttt                                                                                                                >  4:200484/1‑139 (MQ=255)

                                gcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTcc                                                                                                            <  1:25856/139‑1 (MQ=255)

                                     gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               >  6:191336/1‑115 (MQ=255)

                                     gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGAc                                                                                                        >  7:93474/1‑138 (MQ=255)

                                     gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGGTTAATCTCTTTTTCCAGAc                                                                                                        >  2:38365/1‑138 (MQ=255)

                                     gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCAAGATGGAAAGAGAGACCTGCCAGCGTCTCGTGGTTAGCTTCAATGTTGAATTTAATATATTTTTACatca                                                                                                        >  8:326094/1‑135 (MQ=255)

                                      ttGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGGTGGCGTCAATGGTCAGTTTAATCTGTTTTTCCAGAcc                                                                                                       >  3:161452/1‑138 (MQ=255)

                                        gcgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  8:387082/139‑1 (MQ=255)

                                        gcgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCAAGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  6:322707/139‑1 (MQ=255)

                                        gcgcATCGCCACGGGTGGCGGCGACAGAACGGATCAGGGCAAGCGCTATGGCGGTGGCTATTTCATGATCGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATTTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  6:29657/139‑1 (MQ=255)

                                         ggcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  7:254296/137‑1 (MQ=255)

                                         cgcATCGCCACGGTTGGCGTCGACAGTACCGAACAGGCCAAGCTCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  5:270276/138‑1 (MQ=255)

                                         cgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  8:15991/138‑1 (MQ=255)

                                         cgcATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAAc                                                                                                   <  1:133916/139‑1 (MQ=255)

                                           cATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTg                                                                                                 >  2:48879/1‑139 (MQ=255)

                                           cATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATCGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTGAGCTTCAATGTTCAGTTTAATCTGTTTTTACAGACCAAACTg                                                                                                 >  1:100355/1‑139 (MQ=255)

                                                ccACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               <  4:67715/104‑1 (MQ=255)

                                                ccACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCa                                                                                            <  8:77328/139‑1 (MQ=255)

                                                  aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  5:230435/139‑1 (MQ=255)

                                                  aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  3:392498/139‑1 (MQ=255)

                                                  aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  5:75225/139‑1 (MQ=255)

                                                  aCGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  1:8959/139‑1 (MQ=255)

                                                  aCGGGTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  7:349932/139‑1 (MQ=255)

                                                   cGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGGTAGCTTAAATGTTAAGTTTTATCTCTTTTTACAGACCAAACTGtttaacg                                                                                          >  6:39905/1‑134 (MQ=255)

                                                     gTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAg                                                                                       <  4:13961/139‑1 (MQ=255)

                                                      ttGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGc                                                                                      >  8:51228/1‑139 (MQ=255)

                                                      ttGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGGGGTTTTGGTGAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACAGGTTCAGGacat                                                                                      >  2:116234/1‑136 (MQ=255)

                                                         gCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCa                                                                                    <  8:237452/138‑1 (MQ=255)

                                                              gACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  7:153234/127‑1 (MQ=255)

                                                                  gAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcg                                                                          <  3:369828/139‑1 (MQ=255)

                                                                   aaCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcgc                                                                         <  3:320311/139‑1 (MQ=255)

                                                                    aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcgcg                                                                        <  4:268650/139‑1 (MQ=255)

                                                                    aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTCCAGGAAGCCATAGACCGTcgcg                                                                        <  4:371506/139‑1 (MQ=255)

                                                                     ccGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCgg                                                                       <  3:285783/139‑1 (MQ=255)

                                                                        aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAg                                                                                                                                         >  2:317659/1‑70 (MQ=255)

                                                                        aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcgcg                                                                        <  4:327087/135‑1 (MQ=255)

                                                                        aaCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCAt                                                                    <  4:177951/139‑1 (MQ=255)

                                                                            ggCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  6:66751/113‑1 (MQ=255)

                                                                             gCCAAGCGCATTGGCGGTGGCTATTTCTTGATGAAAAGAGTCACCTGCCAGCGGCG‑GTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTaa                                                               <  3:56762/138‑1 (MQ=255)

                                                                             gCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTaa                                                               <  7:126082/139‑1 (MQ=255)

                                                                             gCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGACGCGGCCTCGTaa                                                               >  1:321408/1‑139 (MQ=255)

                                                                                  gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGtt                                                                                                                               >  2:225125/1‑70 (MQ=255)

                                                                                  gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTGAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCata                                                          >  8:127721/1‑139 (MQ=255)

                                                                                  gcgcAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCata                                                          <  1:167191/139‑1 (MQ=255)

                                                                                       aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTaa                                                                                                                       <  6:166969/73‑1 (MQ=255)

                                                                                       aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCCGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGGAATCATATTGAt                                                     >  6:349275/1‑139 (MQ=255)

                                                                                       aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCaaa                                                                                                    <  7:69720/92‑1 (MQ=255)

                                                                                       aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          <  5:200154/102‑1 (MQ=255)

                                                                                       aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTaa                                                               >  2:77662/1‑129 (MQ=255)

                                                                                             gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTTGCTTCAATGTTGATTTTAATCTCTTTTTACAGACCAAACTGTTTCATGAAGCCCTAGACCGTGGCGGGCTCGTAAAAATATTGTTGgtttg                                               >  5:36197/1‑137 (MQ=255)

                                                                                             gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAAtc                                                                                                                     >  4:157404/1‑69 (MQ=255)

                                                                                             gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          >  8:292805/1‑96 (MQ=255)

                                                                                             gTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTgg                                               >  7:378662/1‑139 (MQ=255)

                                                                                              tGGCTATTTCATGATGGATAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCTGACCATACTGTTTCAGGAAGCCTTAGACCGTCGCGGAATCGTAATCATATTGATGTTTGGt                                              <  7:287734/139‑1 (MQ=255)

                                                                                              tGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGt                                              <  7:320476/139‑1 (MQ=255)

                                                                                               ggCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTc                                             <  2:289976/139‑1 (MQ=255)

                                                                                                gCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAgg                                                                                          >  3:187599/1‑93 (MQ=255)

                                                                                                  tATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGt                                          >  5:174017/1‑139 (MQ=255)

                                                                                                    tttCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGGTTAATCTCGTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTGGGTTc                                        >  3:8321/1‑139 (MQ=255)

                                                                                                                     tGACCTGCCAGCGTCGCGTGGTTTTCTTCAAATTTAAGTTTAAACTCATTTTACACACCAACAGGTTTCAGCGAGCCATTAAACGTCGCGCGATCTGAATAATATTTATGTTTGGTGTGTTCTTGCGGTTTCGGTTCGa                       >  6:22393/1‑139 (MQ=255)

                                                                                                                      gACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGa                       >  5:148291/1‑138 (MQ=255)

                                                                                                                       aCCTGCCAGCGTCGCGTGGTTAGCATCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTAGAt                      >  1:20676/1‑138 (MQ=255)

                                                                                                                         cTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGGTCGATAAg                   >  3:258982/1‑139 (MQ=255)

                                                                                                                                 gTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGc           <  5:47047/139‑1 (MQ=255)

                                                                                                                                     cgTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTACAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTg       >  6:138334/1‑139 (MQ=255)

                                                                                                                                         tttAGCTTCTATGTTCAGTTTAATCTCTTTATCCAGACCAAACTGTCTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGGTTCGGTTCGATAAGCAACGTGCCCTGGaaa   <  6:257903/138‑1 (MQ=255)

                                                                                                                                         gTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaaa   <  7:178503/139‑1 (MQ=255)

                                                                                                                                          ttAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAAc  <  2:343956/139‑1 (MQ=255)

TCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAAC  >  pSIJ663_pHT315_araE_xy/8486‑8762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`