Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,359,209 T→C 5.5% L54L (TTA→CTA)  U712_06950 → Magnesium transporter mgtE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,359,2090TC 5.5% 218.1 / 3.8 72L54L (TTA→CTA) U712_06950Magnesium transporter mgtE
Reads supporting (aligned to +/- strand):  ref base T (40/28);  new base C (2/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

ACGAACTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTGGCGATGAACAAAATGGACAACGATGA  >  CP006881/1359072‑1359339
                                                                                                                                         |                                                                                                                                  
aCGAACTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTAtt                                                                                                                                    <  1:72181/138‑1 (MQ=255)
    aCTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAACAACATCGCGCCCGCTATTTATCt                                                                                                                               >  4:230396/1‑139 (MQ=255)
    aCTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCt                                                                                                                               >  7:81277/1‑139 (MQ=255)
    aCTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCt                                                                                                                               >  1:89894/1‑139 (MQ=255)
         ttttACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTAttt                                                                                                                          <  3:75671/139‑1 (MQ=255)
         ttttACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTAttt                                                                                                                          <  1:247867/139‑1 (MQ=255)
            tACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTaa                                                                                                                        <  3:191447/138‑1 (MQ=255)
                catcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTg                                                                                                                     >  1:24086/1‑137 (MQ=255)
                catcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTa                                                                                                                   <  7:230103/139‑1 (MQ=255)
                aatcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTATAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTAATTTTTAAAGAAATGCGGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTa                                                                                                                   <  7:31666/138‑1 (MQ=255)
                  tcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAg                                                                                                                  >  1:219128/1‑138 (MQ=255)
                  tcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGa                                                                                                                 >  1:81261/1‑139 (MQ=255)
                  tcatTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGa                                                                                                                 <  7:150093/139‑1 (MQ=255)
                  taataattTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAg                                                                                                                  >  8:379176/6‑138 (MQ=255)
                    atttttTTACTGCGTGACGGGAAAATCATGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTTTGACATGGCATTTATTTTTAATTAAATGCCGG‑AAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGa                                                                                                              <  5:371953/139‑1 (MQ=255)
                     ttattTTCCTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGa                                                                                                              <  6:170248/139‑1 (MQ=255)
                           tACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGTAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCa                                                                                                         <  1:160585/138‑1 (MQ=255)
                           tACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCa                                                                                                         <  7:379176/138‑1 (MQ=255)
                           tACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCa                                                                                                         <  3:131470/138‑1 (MQ=255)
                           tACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCa                                                                                                         <  8:81277/138‑1 (MQ=255)
                           tACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCAc                                                                                                        >  3:265919/1‑139 (MQ=255)
                             cTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGa                                                                                                                 >  8:190596/1‑128 (MQ=255)
                             cTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGa                                                                                                                 <  7:190596/128‑1 (MQ=255)
                             cTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACt                                                                                                       >  2:79943/1‑138 (MQ=255)
                             cTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCACTATGACATGGCATTTATTTTTAAAGAAATGACGGAAAAACATCGCGCCCGCTATTTATATTATTTAACTGTAGACGATATCACt                                                                                                       >  2:323557/1‑138 (MQ=255)
                              ggcgcGACGGGAAAATCAGGTATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCGGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGa                                                                                                     <  1:172108/138‑1 (MQ=255)
                                cGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGata                                                                                                   >  1:56312/1‑139 (MQ=255)
                                cGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGata                                                                                                   >  5:74506/1‑139 (MQ=255)
                                      gggAACATCAGGTATTTTAGAAGTTTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCg                                                                                             <  4:265919/139‑1 (MQ=255)
                                      gggAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCg                                                                                             <  2:219128/139‑1 (MQ=255)
                                                               ttATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGc                                                                     <  2:89894/138‑1 (MQ=255)
                                                                 aTTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTg                                                                  >  4:234661/1‑139 (MQ=255)
                                                                  ttGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTg                                                                  <  6:329308/138‑1 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACTTCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCt                                                            >  5:373907/1‑139 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTgtcgtc                                                             <  1:79943/138‑1 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATCTa                                                                                                                                  <  1:124536/69‑1 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATCTa                                                                                                                                  >  2:118658/1‑69 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATCTa                                                                                                                                  <  1:118658/69‑1 (MQ=255)
                                                                       gaGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATCTa                                                                                                                                  >  2:124536/1‑69 (MQ=255)
                                                                          cTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTta                                                          >  6:113028/1‑138 (MQ=255)
                                                                          cTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGATATTTATCTTATATAACTGTATACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTta                                                          >  4:83204/1‑138 (MQ=255)
                                                                               gCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTtaaata                                                      >  8:69188/1‑137 (MQ=255)
                                                                                cccTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTaa                                                                                                                        <  3:367965/70‑1 (MQ=255)
                                                                                cccTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTaa                                                                                                                        >  4:367965/1‑70 (MQ=255)
                                                                                   tATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAATGTct                                                >  2:385299/1‑138 (MQ=255)
                                                                                    aTGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCg                                                >  3:180999/1‑138 (MQ=255)
                                                                                     tGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCgg                                               >  4:268417/1‑138 (MQ=255)
                                                                                     tGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGa                                              <  5:142436/139‑1 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGACCGCGAATTTCAGCTTGTCGTGTTAAATAAAGTCGGaaaaa                                          >  1:401719/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATTAAGTCGGaaaaa                                          >  5:341844/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGaaaaa                                          >  2:253956/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGaaaaa                                          >  2:241501/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGaaaaa                                          >  8:21927/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGaaaaa                                          >  7:215340/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGaaaaa                                          >  3:59780/1‑138 (MQ=255)
                                                                                          tGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAAc                                         <  5:113028/139‑1 (MQ=255)
                                                                                           ggtatttttttTTAAATAAATGCCGTATATACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGTGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAATTAAAGTCGGAAAAAc                                         <  1:323557/135‑1 (MQ=255)
                                                                                           ggCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTg                                                                  >  6:18368/1‑113 (MQ=255)
                                                                                           ggCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTg                                                                  <  5:18368/113‑1 (MQ=255)
                                                                                           ggCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAAc                                         >  6:124282/1‑138 (MQ=255)
                                                                                            gCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCGATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGACGTCTTAAATAAAGTCGGAAAAACa                                        >  1:3773/1‑138 (MQ=255)
                                                                                             cATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACaa                                       >  1:377452/1‑138 (MQ=255)
                                                                                               tttatttTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAg                                    >  3:115595/1‑139 (MQ=255)
                                                                                               tttatttTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAg                                    >  5:379484/1‑139 (MQ=255)
                                                                                                  atttTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCac                                 >  7:252358/1‑138 (MQ=255)
                                                                                                    ttttAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAaca                               >  5:369777/1‑139 (MQ=255)
                                                                                                      ttAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACaaa                                      >  7:250708/1‑130 (MQ=255)
                                                                                                      ttAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACaaa                                      <  8:250708/130‑1 (MQ=255)
                                                                                                       tAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTg                            >  8:352655/1‑139 (MQ=255)
                                                                                                                   ggAAAAAAACCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTGGCGATGAACaaa                <  3:43652/139‑1 (MQ=255)
                                                                                                                    aaaaaaaATCGCGCCCTATTTTTATTTTATTTAAGTGTAGACGAAATCACTTATATGTTCGGGGAGCTGTACCCCTATTTTCAGTTTTTGGTCTTAAAAAATGTCGCAAAAAAAAAAGCAACACTGGCGATGAACaaa                <  6:341844/137‑1 (MQ=255)
                                                                                                                               cgcCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTGGCGATGAACAAAATGGACAACGAt    >  5:71997/1‑139 (MQ=255)
                                                                                                                                 cccGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTGGCGATGAACAAAATGGACAACGATGa  <  6:71997/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
ACGAACTCATTTTACGCATCATTATTTTACTGCGAGACGGGAAAATCAGGGATTTTAGAAGTGTTATTGATGAGCTTCAGCCCTATGACATGGCATTTATTTTTAAAGAAATGCCGGAAAAACATCGCGCCCGCTATTTATCTTATTTAACTGTAGACGATATCACTGATATGATCGGGGAGCTGGAACGCGAATTTCAGCTTGTCGTCTTAAATAAAGTCGGAAAAACAAAAGCAACACTGGCGATGAACAAAATGGACAACGATGA  >  CP006881/1359072‑1359339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑