| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,604,910 | C→T | 8.0% | A67A (GCC→GCT) | U712_08250 → | Hypothetical Protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,604,910 | 0 | C | T | 8.0% | 192.9 / 5.1 | 62 | A67A (GCC→GCT) | U712_08250 | Hypothetical Protein |
| Reads supporting (aligned to +/- strand): ref base C (25/32); new base T (3/2); total (28/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
GTCAGCCCGGAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTAAAGGAACGGT > CP006881/1604776‑1605048 | gTCAGCCCGGAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTc < 6:131495/138‑1 (MQ=255)gTCAGCCCGGAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTc < 6:299447/138‑1 (MQ=255) gCCCGGAGTCTGCGCCACTCAAACGGCTGATTCAGGCTGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGcc < 8:342112/139‑1 (MQ=255) cGGAGTCTGAGCCAATCAAACGGATGATTCCGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCGga < 5:334176/139‑1 (MQ=255) gAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTgaga < 1:235017/139‑1 (MQ=255) gAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTgaga < 3:207700/139‑1 (MQ=255) gAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTgaga < 2:336644/139‑1 (MQ=255) cTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGt < 5:375558/139‑1 (MQ=255) gcgcCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTg > 4:16147/1‑139 (MQ=255) gcgcCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTg > 3:298202/1‑139 (MQ=255) cgcCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGt < 3:7131/136‑1 (MQ=255) cgcCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGc > 8:24015/1‑139 (MQ=255) gcCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTCTCTTATCTGCCGTCCAGCCTGAGACAGTTGca < 7:90110/139‑1 (MQ=255) cAATCAAACGGATGATTCAGGATGCAAGAGACAGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGcaca < 2:50944/139‑1 (MQ=255) aaTCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTc > 5:333143/1‑118 (MQ=255) aaTCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTc < 6:333143/118‑1 (MQ=255) tCAAACGGATGATTCAGGATGCAAGAGCCCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAg > 1:29566/1‑139 (MQ=255) gATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAaagaag < 3:16147/139‑1 (MQ=255) aTGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAaagaag > 7:256820/1‑138 (MQ=255) gagaCCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTAt < 7:24015/139‑1 (MQ=255) cgctGAATTCAAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATg < 6:41695/139‑1 (MQ=255) cgcgGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGAt > 4:109847/1‑138 (MQ=255) aaTGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAggg < 7:28044/139‑1 (MQ=255) aTGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAggg < 1:43241/138‑1 (MQ=255) tGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCa < 6:137703/139‑1 (MQ=255) tGCTAATTGACGCTACATACGGAAGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCa < 3:23254/139‑1 (MQ=255) cGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTg > 6:37061/1‑139 (MQ=255) cTACATACGGACGAAGAACCCGAGCAGTTGTCGTCATGGATAGTGATCACATTATCTAATCTGCCGGCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGcc > 1:284621/1‑139 (MQ=255) ggACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATga > 3:264382/1‑137 (MQ=255) ggACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATga > 1:178527/1‑137 (MQ=255) ccgcagaaCCCGTGCAGTTGTCGTCATGGAGAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAATAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATgaaaga < 4:12071/135‑1 (MQ=255) cGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGcc < 1:309769/62‑1 (MQ=255) cGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGcc > 2:309769/1‑62 (MQ=255) cGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGcaca < 1:152634/77‑1 (MQ=255) cGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGcaca > 2:152634/1‑77 (MQ=255) cGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATgaaaga > 8:39075/1‑138 (MQ=255) aagaaCCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATgaaaga > 6:161411/1‑136 (MQ=255) aagaaCCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGaaagaaag < 5:37061/139‑1 (MQ=255) agaaCCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAaagaaaga < 5:112659/139‑1 (MQ=255) gaaCCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTAt < 5:141567/100‑1 (MQ=255) gaaCCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTAt > 6:141567/1‑100 (MQ=255) tGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGttat > 2:28628/1‑138 (MQ=255) tGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGttat > 7:353459/1‑138 (MQ=255) gTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATc < 8:353459/139‑1 (MQ=255) ttGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTga > 3:47489/1‑49 (MQ=255) ttGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTga < 4:47489/49‑1 (MQ=255) gtcgtcATGGATAGTGATCACATTATCTTATCTGCTGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATCAAAGAAAGAGGGTTATTAATCGtt > 1:100883/1‑139 (MQ=255) aTGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCa < 1:28628/139‑1 (MQ=255) tGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAaagaaaga < 4:113760/110‑1 (MQ=255) tGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAaagaaaga > 3:113760/1‑110 (MQ=255) tGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAaagaaaga < 2:341181/110‑1 (MQ=255) tGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAaagaaaga > 1:341181/1‑110 (MQ=255) tGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTc > 6:387697/1‑139 (MQ=255) acaTTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGGTATTAATCGTTCTCTCAGGTCCCTCAGGa < 8:256820/138‑1 (MQ=255) atcttatctGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTgg > 3:338134/1‑138 (MQ=255) atcttatctGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTgg > 7:334391/1‑138 (MQ=255) atctGCTGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAgg > 7:256181/1‑118 (MQ=255) atctGCTGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAgg > 1:178381/1‑118 (MQ=255) atctGCTGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAgg < 8:256181/118‑1 (MQ=255) atctGCTGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAgg < 2:178381/118‑1 (MQ=255) atctGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTaa < 4:264382/136‑1 (MQ=255) ctGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTAAAGGaa > 1:240717/1‑139 (MQ=255) cGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAACGAGGGTTATTAATCGTTCTCTCAgg < 4:359725/112‑1 (MQ=255) cGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAgg > 3:359725/1‑112 (MQ=255) cGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTAAAGGAACGGt > 4:247917/1‑139 (MQ=255) | GTCAGCCCGGAGTCTGCGCCAATCAAACGGATGATTCAGGATGCAAGAGACCGCGGAATGCTAATTGACGCTACATACGGACGAAGAACCCGTGCAGTTGTCGTCATGGATAGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGAAGGGCAGGGGTAATTGCCGCATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTAAAGGAACGGT > CP006881/1604776‑1605048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |