Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,626,502 G→A 8.9% A306A (GCG→GCA)  U712_08355 → Phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,626,5020GA 8.9% 136.2 / 3.0 45A306A (GCG→GCA) U712_08355Phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (30/11);  new base A (2/2);  total (32/13)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

GCTGTGCTGAAACCAAAATTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAGG  >  CP006881/1626365‑1626638
                                                                                                                                         |                                                                                                                                        
gcTGTGCTGAAACCAAAATTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGa                                                                                                                                         >  4:337804/1‑139 (MQ=255)
 cTGTGCTGAAACCAAAATTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCTAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCCGATTCAAATGCTGTATTCCATGCGAt                                                                                                                                        >  6:377382/1‑139 (MQ=255)
    tgCTGAAACCAAAATTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCg                                                                                                                                     >  5:344640/1‑139 (MQ=255)
      attaaaCAAAAATTGAAATAAATCAAAATTAAATTGGTGTATTCCATTTTGGGCGGAGCAAGTTTTTTTGGCTTAAACGCGCCTGTTATCACGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGgc                                                                                                                                   <  8:101364/136‑1 (MQ=255)
                aaTTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTGCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAaga                                                                                                                           >  2:146425/1‑137 (MQ=255)
                      aaaaaattaaaattaAATTGGAGTATCCTAATTTGGGGGGACAATGTGTTTTTGCCTAAAACGCCCCTGTCTTCACGGCGCACGGCTCCTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGt                                                                                                                   <  6:161701/136‑1 (MQ=255)
                                             tATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTc                                                                                             >  4:268710/1‑138 (MQ=255)
                                             tATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCa                                                                                            >  8:309551/1‑139 (MQ=255)
                                             tATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCa                                                                                            >  6:315751/1‑139 (MQ=255)
                                                       aTGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGgaag                                                                                   >  5:152609/1‑138 (MQ=255)
                                                       aTGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGgaag                                                                                   >  7:187252/1‑138 (MQ=255)
                                                       aTGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCAATTCGTCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGgaag                                                                                   >  8:297391/1‑138 (MQ=255)
                                                       aTGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCAATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGgaag                                                                                   >  8:302885/1‑138 (MQ=255)
                                                         ggCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGaagaag                                                                                >  7:297638/1‑139 (MQ=255)
                                                              aGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGa                                                                             >  2:272778/1‑137 (MQ=255)
                                                                        tttGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGaaaaaa                                                                 >  1:81523/1‑139 (MQ=255)
                                                                                aaaaGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGag                                                         >  7:21161/1‑139 (MQ=255)
                                                                                       ccTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTg                                                  >  7:382418/1‑139 (MQ=255)
                                                                                       ccTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTg                                                  >  7:86062/1‑139 (MQ=255)
                                                                                                     gcACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCTGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  5:315751/139‑1 (MQ=255)
                                                                                                     gcACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAATATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  3:268710/139‑1 (MQ=255)
                                                                                                     gcACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  8:86062/139‑1 (MQ=255)
                                                                                                     gcACGGCTCTTCAGATTCAAATGCTGTATTCCATGCAATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  7:297391/139‑1 (MQ=255)
                                                                                                     gcACGGCTCTTCAGATTCAAATGCTGTATTCCATGCAATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  7:302885/139‑1 (MQ=255)
                                                                                                     ccACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatc                                    <  7:70522/138‑1 (MQ=255)
                                                                                                       aCGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTAcatcat                                  >  3:68532/1‑139 (MQ=255)
                                                                                                                  gATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTg                       <  3:213959/139‑1 (MQ=255)
                                                                                                                     tCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCAt                    >  6:202935/1‑139 (MQ=255)
                                                                                                                     tCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCAt                    >  2:391092/1‑139 (MQ=255)
                                                                                                                     tCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCAt                    >  7:333743/1‑139 (MQ=255)
                                                                                                                             tGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTc            >  8:316374/1‑139 (MQ=255)
                                                                                                                               tATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCGCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTcc           <  7:327851/138‑1 (MQ=255)
                                                                                                                                     ctGCGATTCGGCAAGCAAGATAGATGGTCACCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGAGGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCa    <  5:228676/138‑1 (MQ=255)
                                                                                                                                     aTGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGt      >  3:361194/1‑137 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGAt                                                            >  1:188275/1‑82 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGAt                                                            <  2:188275/82‑1 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGc                      <  2:383251/120‑1 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGc                      >  1:383251/1‑120 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAg   >  7:350760/1‑139 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAg   >  5:216561/1‑139 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAg   >  2:138784/1‑139 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAg   >  6:186443/1‑139 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAg   >  5:45391/1‑139 (MQ=255)
                                                                                                                                      tGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGGCAg   >  5:43400/1‑139 (MQ=255)
                                                                                                                                       gCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAgg  >  8:154954/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCTGTGCTGAAACCAAAATTGAAAGAAATGAAAATGAAAATGGAGTATTCGAATTATGGCGGAGCAAGTCTTTTTGGCTTAAAAGCGCCTGTGATCAAGGCGCACGGCTCTTCAGATTCAAATGCTGTATTCCATGCGATTCGGCAAGCAAGAGAGATGGTCAGCCAAAATGTGGCTGCGCTCATTCAGGAAGAAGTGAAAGAAGAAAAAACAGATGAGTAGTCTGGAGGTTTTTACATCATGAGTAAGATTGCATTTTTATTCCCGGGTCAGG  >  CP006881/1626365‑1626638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑