Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,044,471 A→G 8.6% N171S (AAT→AGT)  U712_10115 → Transcriptional regulatory protein desR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,044,4710AG 8.6% 131.3 / 3.8 50N171S (AAT→AGT) U712_10115Transcriptional regulatory protein desR
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGAGGACTTATACAGCGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGATCCTTTTTGTT  >  CP006881/2044341‑2044595
                                                                                                                                  |                                                                                                                            
aTGGAGGACTTATACAGCGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATAtct                                                                                                                      >  7:85176/1‑139 (MQ=255)
aTGGAGGACTTATACAGCGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATAtct                                                                                                                      >  4:344216/1‑139 (MQ=255)
    aggtCTTATACAGCGAAGGAAACCCTCTTACAGTTAGAGAAAAAGATGTGCTCGATCTTGTGGCTGTCGGTAAAAACACAAAAGAAATCGCTCAGGAAGTGAGCATCAAAAGCGGGACGGTGCGGATTTATATCTCAAt                                                                                                                  <  6:201739/139‑1 (MQ=255)
          tataCAGCGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTc                                                                                                             >  2:280094/1‑138 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATCGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGaa                                                                                                                              <  3:276298/114‑1 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGaa                                                                                                                              >  4:276298/1‑114 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGAtt                                                                                                              <  2:390689/130‑1 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGAtt                                                                                                              >  1:390689/1‑130 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAg                                                                                                     >  2:205883/1‑139 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAg                                                                                                     >  5:5101/1‑139 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAg                                                                                                     >  6:288150/1‑139 (MQ=255)
                 cGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAg                                                                                                     >  7:229472/1‑139 (MQ=255)
                   aaGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAAt                                                                                                                  <  3:299001/124‑1 (MQ=255)
                   aaGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAAt                                                                                                                  >  4:299001/1‑124 (MQ=255)
                   aaGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCt                                                                                                   >  7:207120/1‑139 (MQ=255)
                   aaGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGT‑GCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGAGGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCtt                                                                                                  >  4:407130/1‑139 (MQ=255)
                                     aTAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCCATGATTCTAGAAAAGCTTGAAGAGAAAAATCGAAt                                                                                 >  8:65555/1‑139 (MQ=255)
                                     aTAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAAt                                                                                 >  8:43148/1‑139 (MQ=255)
                                      tAGAGAAAAAGAAGTGCTCGAACTTGT‑GCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               <  3:407130/139‑1 (MQ=255)
                                       agagAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGCACTCAGCATCAAAAGCGGGACGGTGCGGATTTATATCTCAATGCTTCTAGAAAAGCTTGAAGTGAAAAATCGAAtt                                                                                >  3:96561/1‑138 (MQ=255)
                                       agagAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAAtt                                                                                >  2:26571/1‑138 (MQ=255)
                                        tagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               <  4:116407/137‑1 (MQ=255)
                                        gagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               <  8:389384/138‑1 (MQ=255)
                                        gagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               <  8:59241/138‑1 (MQ=255)
                                        gagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               <  8:52187/138‑1 (MQ=255)
                                        gagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGa                                                                              <  2:108553/139‑1 (MQ=255)
                                        gagaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGa                                                                              <  8:207120/139‑1 (MQ=255)
                                         agaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               >  4:338776/1‑137 (MQ=255)
                                         agaAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTg                                                                               >  7:243863/1‑137 (MQ=255)
                                             aaaGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCa                                                                         <  1:280094/139‑1 (MQ=255)
                                             aaaGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCa                                                                         <  1:205883/139‑1 (MQ=255)
                                                  agTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAACGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTAcc                                                                    <  4:224667/139‑1 (MQ=255)
                                                   gTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTAccc                                                                   >  3:281083/1‑139 (MQ=255)
                                                         gAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCa                                                              <  8:243863/138‑1 (MQ=255)
                                                                     gACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCt                                                 >  8:120041/1‑139 (MQ=255)
                                                                                  caAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATaaaa                                    <  5:288150/139‑1 (MQ=255)
                                                                                   aaaaGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATaaaaa                                   <  3:338776/139‑1 (MQ=255)
                                                                                   aaaaGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATaaaaa                                   <  3:344216/139‑1 (MQ=255)
                                                                                              cTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGc                        <  8:90314/139‑1 (MQ=255)
                                                                                              cTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGc                        <  4:338117/139‑1 (MQ=255)
                                                                                              cTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGc                        <  4:281083/139‑1 (MQ=255)
                                                                                               tCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCa                       >  2:117806/1‑139 (MQ=255)
                                                                                                cAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCAt                      <  7:43148/139‑1 (MQ=255)
                                                                                                   gAACTCAGCATCAAAAGCGGGACGGTGCGGAGTTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAgg                                                   <  4:123871/107‑1 (MQ=255)
                                                                                                   gAACTCAGCATCAAAAGCGGGACGGTGCGGAGTTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAgg                                                   <  4:118040/107‑1 (MQ=255)
                                                                                                   gAACTCAGCATCAAAAGCGGGACGGTGCGGAGTTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAgg                                                   >  3:123871/1‑107 (MQ=255)
                                                                                                   gAACTCAGCATCAAAAGCGGGACGGTGCGGAGTTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAgg                                                   >  3:118040/1‑107 (MQ=255)
                                                                                                         aGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAgg              <  1:26571/138‑1 (MQ=255)
                                                                                                         aGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGa             >  4:180381/1‑139 (MQ=255)
                                                                                                                aaaGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGATCCtttt      >  6:148744/1‑139 (MQ=255)
                                                                                                                   gCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGATCCTTTTTGt   >  8:136580/1‑139 (MQ=255)
                                                                                                                     gggACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGATCCTTTTTGtt  >  1:176852/1‑138 (MQ=255)
                                                                                                                                  |                                                                                                                            
ATGGAGGACTTATACAGCGAAGCTAACCCTCTTACAGATAGAGAAAAAGAAGTGCTCGAACTTGTGGCTGACGGTAAAAACACAAAAGAAATCGCTCAGGAACTCAGCATCAAAAGCGGGACGGTGCGGAATTATATCTCAATGATTCTAGAAAAGCTTGAAGTGAAAAATCGAATTGAAGCCATTACCCGGTCAAAAGAAAAAGGCTGGTTTAAATAAAAAAGGATCTTGGCATCTGCCAGGATCCTTTTTGTT  >  CP006881/2044341‑2044595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑